pLKO.1-U6-shRNA-Negative-Contr

pLKO.1-U6-shRNA-Negative-Control-Hyg plasmid

PVT111815

pLKO.1-U6-shRNA-Negative-Control-Hyg plasmid Information

Plasmid name: pLKO.1-U6-shRNA-Negative-Control-Hyg
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene interference,negative control
Fragment type: shRNA
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLKO.1-U6-shRNA-Negative-Control-Hyg plasmid Description

pLKO.1-U6-shRNA-Negative-Control-Hyg is a plasmid suitable for gene interference in mammalian cells, serving as a negative control, and can also be used for lentivirus packaging.

pLKO.1-U6-shRNA-Negative-Control-Hyg plasmid Sequence

1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgCAGCTGGC 61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG 241 CAATTAACCC TCACTAAAGG GAACAAAAGC TGGAGCTGCA AGCTTAATGT AGTCTTATGC 301 AATACTCTTG TAGTCTTGCA ACATGGTAAC GATGAGTTAG CAACATGCCT TACAAGGAGA 361 GAAAAAGCAC CGTGCATGCC GATTGGTGGA AGTAAGGTGG TACGATCGTG CCTTATTAGG 421 AAGGCAACAG ACGGGTCTGA CATGGATTGG ACGAACCACT GAATTGCCGC ATTGCAGAGA 481 TATTGTATTT AAGTGCCTAG CTCGATACAT AAACGGGTCT CTCTGGTTAG ACCAGATCTG 541 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC 601 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT 661 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA 721 GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG 781 GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG 841 AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG 901 GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG 961 GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC 1021 TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA 1081 TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC 1141 ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG 1201 CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA 1261 ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA 1321 GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT 1381 CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG ACGCTGACGG TACAGGCCAG 1441 ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA TTGAGGCGCA 1501 ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC 1561 TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT 1621 CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT 1681 TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT 1741 ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA 1801 ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT 1861 AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT 1921 TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA CCCACCTCCC 1981 AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG AGAGAGACAG 2041 AGACAGATCC ATTCGATTAG TGAACGGATC TCGACGGTAT CGATCACGAG ACTAGCCTCG 2101 AGCGGCCGCC CCCTTCACCG AGGGCCTATT TCCCATGATT CCTTCATATT TGCATATACG 2161 ATACAAGGCT GTTAGAGAGA TAATTGGAAT TAATTTGACT GTAAACACAA AGATATTAGT 2221 ACAAAATACG TGACGTAGAA AGTAATAATT TCTTGGGTAG TTTGCAGTTT TAAAATTATG 2281 TTTTAAAATG GACTATCATA TGCTTACCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2341 ATATATCTTG TGGAAAGGAC GAAACACCGG CAACAAGATG AAGAGCACCA ACTTCAAGAG 2401 AGTTGGTGCT CTTCATCTTG TTGttttttG AATTCTCGAC CTCGAGACAA ATGGCAGTAT 2461 TCATCCACAA TTTTAAAAGA AAAGGGGGGA TTGGGGGGTA CAGTGCAGGG GAAAGAATAG 2521 TAGACATAAT AGCAACAGAC ATACAAACTA AAGAATTACA AAAACAAATT ACAAAAATTC 2581 AAAATTTTCG GGTTTATTAC AGGGACAGCA GAGATCCACT TTGGCCGCGG CTCGAGGGGG 2641 TTGGGGTTGC GCCTTTTCCA AGGCAGCCCT GGGTTTGCGC AGGGACGCGG CTGCTCTGGG 2701 CGTGGTTCCG GGAAACGCAG CGGCGCCGAC CCTGGGTCTC GCACATTCTT CACGTCCGTT 2761 CGCAGCGTCA CCCGGATCTT CGCCGCTACC CTTGTGGGCC CCCCGGCGAC GCTTCCTGCT 2821 CCGCCCCTAA GTCGGGAAGG TTCCTTGCGG TTCGCGGCGT GCCGGACGTG ACAAACGGAA 2881 GCCGCACGTC TCACTAGTAC CCTCGCAGAC GGACAGCGCC AGGGAGCAAT GGCAGCGCGC 2941 CGACCGCGAT GGGCTGTGGC CAATAGCGGC TGCTCAGCAG GGCGCGCCGA GAGCAGCGGC 3001 CGGGAAGGGG CGGTGCGGGA GGCGGGGTGT GGGGCGGTAG TGTGGGCCCT GTTCCTGCCC 3061 GCGCGGTGTT CCGCATTCTG CAAGCCTCCG GAGCGCACGT CGGCAGTCGG CTCCCTCGTT 3121 GACCGAATCA CCGACCTCTC TCCCCAGGGG GATCCACCGG ATCAAGCTTG CCAGCTGGGG 3181 CGCCCTCTGG TAAGGTTGGG AAGCCCTGCA GATCTGATCC CGGGGGGCAA TGAGATATGA 3241 AAAAGCCTGA ACTCACCGCG ACGTCTGTCG AGAAGTTTCT GATCGAAAAG TTCGACAGCG 3301 TCTCCGACCT GATGCAGCTC TCGGAGGGCG AAGAATCTCG TGCTTTCAGC TTCGATGTAG 3361 GAGGGCGTGG ATATGTCCTG CGGGTAAATA GCTGCGCCGA TGGTTTCTAC AAAGATCGTT 3421 ATGTTTATCG GCACTTTGCA TCGGCCGCGC TCCCGATTCC GGAAGTGCTT GACATTGGGG 3481 AATTTAGCGA GAGCCTGACC TATTGCATCT CCCGCCGTGC ACAGGGTGTC ACGTTGCAAG 3541 ACCTGCCTGA AACCGAACTG CCCGCTGTTC TGCAGCCGGT CGCGGAGGCC ATGGATGCGA 3601 TCGCTGCGGC CGATCTTAGC CAGACGAGCG GGTTCGGCCC ATTCGGACCG CAAGGAATCG 3661 GTCAATACAC TACATGGCGT GATTTCATAT GCGCGATTGC TGATCCCCAT GTGTATCACT 3721 GGCAAACTGT GATGGACGAC ACCGTCAGTG CGTCCGTCGC GCAGGCTCTC GATGAGCTGA 3781 TGCTTTGGGC CGAGGACTGC CCCGAAGTCC GGCACCTCGT GCACGCGGAT TTCGGCTCCA 3841 ACAATGTCCT GACGGACAAT GGCCGCATAA CAGCGGTCAT TGACTGGAGC GAGGCGATGT 3901 TCGGGGATTC CCAATACGAG GTCGCCAACA TCTTCTTCTG GAGGCCGTGG TTGGCTTGTA 3961 TGGAGCAGCA GACGCGCTAC TTCGAGCGGA GGCATCCGGA GCTTGCAGGA TCGCCGCGGC 4021 TCCGGGCGTA TATGCTCCGC ATTGGTCTTG ACCAACTCTA TCAGAGCTTG GTTGACGGCA 4081 ATTTCGATGA TGCAGCTTGG GCGCAGGGTC GATGCGACGC AATCGTCCGA TCCGGAGCCG 4141 GGACTGTCGG GCGTACACAA ATCGCCCGCA GAAGCGCGGC CGTCTGGACC GATGGCTGTG 4201 TAGAAGTACT CGCCGATAGT GGAAACCGAC GCCCCAGCAC TCGTCCGAGG GCAAAGGAAT 4261 AGAGTAGATG CCGACCGGGA TCTATCGATA CCGTCGACCT CGAGGGGGGG CCCGGTACCT 4321 TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG 4381 GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATC TGCTTTTTGC TTGTACTGGG 4441 TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG 4501 CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT 4561 GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT 4621 AGTAGTTCAT GTCATCTTAT TATTCAGTAT TTATAACTTG CAAAGAAATG AATATCAGAG 4681 AGTGAGAGGA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA 4741 AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC 4801 AATGTATCTT ATCATGTCTG GCTCTAGCTA TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 4861 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG 4921 CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 4981 GAGGCCTAGG GACGTACCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC 5041 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC 5101 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC 5161 CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGCG CATTAAGCGC 5221 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC 5281 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT 5341 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA 5401 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC 5461 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT 5521 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 5581 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT 5641 TACAATTTAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC 5701 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA 5761 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT 5821 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT 5881 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC 5941 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA 6001 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC 6061 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC 6121 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC 6181 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG 6241 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC 6301 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC 6361 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT 6421 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA 6481 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC 6541 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG 6601 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA 6661 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC 6721 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA 6781 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 6841 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA 6901 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT 6961 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC 7021 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG 7081 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 7141 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 7201 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 7261 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 7321 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG 7381 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 7441 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT 7501 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA 7561 GTGAGCGAGG AAGCGGAAG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.