pLV3-CMV-LRRC4C-3×FLAG-Fluc-Pu

pLV3-CMV-LRRC4C-3×FLAG-Fluc-Puro plasmid

PVT111800

pLV3-CMV-LRRC4C-3×FLAG-Fluc-Puro plasmid Information

Plasmid name: pLV3-CMV-LRRC4C(human)-3×FLAG-Fluc-Puro
Alias: NM_020929.3;NGL-1; NGL1
Gene lenght: 1920
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-LRRC4C-3×FLAG-Fluc-Puro plasmid Description

pLV3-CMV-LRRC4C-3×FLAG-Fluc-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-LRRC4C-3×FLAG-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgt tgaacaagat gaccttacat ccacagcaga taatgatagg 2341 tcctaggttt aacagggccc tatttgaccc cctgcttgtg gtgctgctgg ctcttcaact 2401 tcttgtggtg gctggtctgg tgcgggctca gacctgccct tctgtgtgct cctgcagcaa 2461 ccagttcagc aaggtgattt gtgttcggaa aaacctgcgt gaggttccgg atggcatctc 2521 caccaacaca cggctgctga acctccatga gaaccaaatc cagatcatca aagtgaacag 2581 cttcaagcac ttgagacact tggaaatcct acagttgagt aggaaccata tcagaaccat 2641 tgaaattggg gctttcaatg gtctggcgaa cctcaacact ctggaactct ttgacaatcg 2701 tcttactacc atcccgaatg gagcttttgt atacttgtct aaactgaagg agctctggtt 2761 gcgaaacaac cccattgaaa gcatcccttc ttatgctttt aacagaattc cttctttgcg 2821 ccgactagac ttaggggaat tgaaaagact ttcatacatc tcagaaggtg cctttgaagg 2881 tctgtccaac ttgaggtatt tgaaccttgc catgtgcaac cttcgggaaa tccctaacct 2941 cacaccgctc ataaaactag atgagctgga tctttctggg aatcatttat ctgccatcag 3001 gcctggctct ttccagggtt tgatgcacct tcaaaaactg tggatgatac agtcccagat 3061 tcaagtgatt gaacggaatg cctttgacaa ccttcagtca ctagtggaga tcaacctggc 3121 acacaataat ctaacattac tgcctcatga cctcttcact cccttgcatc atctagagcg 3181 gatacattta catcacaacc cttggaactg taactgtgac atactgtggc tcagctggtg 3241 gataaaagac atggccccct cgaacacagc ttgttgtgcc cggtgtaaca ctcctcccaa 3301 tctaaagggg aggtacattg gagagctcga ccagaattac ttcacatgct atgctccggt 3361 gattgtggag ccccctgcag acctcaatgt cactgaaggc atggcagctg agctgaaatg 3421 tcgggcctcc acatccctga catctgtatc ttggattact ccaaatggaa cagtcatgac 3481 acatggggcg tacaaagtgc ggatagctgt gctcagtgat ggtacgttaa atttcacaaa 3541 tgtaactgtg caagatacag gcatgtacac atgtatggtg agtaattccg ttgggaatac 3601 tactgcttca gccaccctga atgttactgc agcaaccact actcctttct cttacttttc 3661 aaccgtcaca gtagagacta tggaaccgtc tcaggatgag gcacggacca cagataacaa 3721 tgtgggtccc actccagtgg tcgactggga gaccaccaat gtgaccacct ctctcacacc 3781 acagagcaca aggtcgacag agaaaacctt caccatccca gtgactgata taaacagtgg 3841 gatcccagga attgatgagg tcatgaagac taccaaaatc atcattgggt gttttgtggc 3901 catcacactc atggctgcag tgatgctggt cattttctac aagatgagga agcagcacca 3961 tcggcaaaac catcacgccc caacaaggac tgttgaaatt attaatgtgg atgatgagat 4021 tacgggagac acacccatgg aaagccacct gcccatgcct gctatcgagc atgagcacct 4081 aaatcactat aactcataca aatctccctt caaccacaca acaacagtta acacaataaa 4141 ttcaatacac agttcagtgc atgaaccgtt attgatccga atgaactcta aagacaatgt 4201 acaagagact caaatcggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA 4261 CATCGACTAC AAGGATGACG ATGACAAGTA GTGAgcggcc gcaaggatct gcgatcgctc 4321 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 4381 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 4441 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 4501 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 4561 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 4621 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 4681 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 4741 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 4801 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac 4861 catggagagc gacgagagcg gcctgatgga agacgccaaa aacataaaga aaggcccggc 4921 gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag 4981 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac 5041 ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct 5101 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt 5161 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg 5221 tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg 5281 gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat 5341 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct 5401 acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat 5461 tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca 5521 tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat 5581 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac 5641 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct 5701 gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt 5761 ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat 5821 tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca 5881 tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac 5941 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa 6001 ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt 6061 gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat 6121 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt 6181 cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc 6241 tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct 6301 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac 6361 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt 6421 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc 6481 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgagatc 6541 ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc 6601 tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat 6661 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 6721 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 6781 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 6841 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 6901 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 6961 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 7021 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 7081 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 7141 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 7201 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa 7261 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 7321 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 7381 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 7441 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 7501 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 7561 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 7621 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 7681 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 7741 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg 7801 ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag 7861 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 7921 ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct 7981 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 8041 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 8101 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 8161 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 8221 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 8281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 8341 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct 8401 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 8461 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc 8521 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 8581 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 8641 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 8701 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 8761 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 8821 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 8881 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 8941 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 9001 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 9061 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 9121 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 9181 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 9241 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 9301 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 9361 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 9421 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 9481 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 9541 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 9601 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 9661 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 9721 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 9781 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 9841 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 9901 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 9961 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 10021 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 10081 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 10141 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 10201 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 10261 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 10321 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 10381 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 10441 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 10501 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 10561 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 10621 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 10681 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 10741 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 10801 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 10861 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 10921 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 10981 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg 11041 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 11101 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag 11161 ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.