pLV3-CMV-Tert-3×FLAG-CopGFP-Pu

pLV3-CMV-Tert-3×FLAG-CopGFP-Puro plasmid

PVT111712

pLV3-CMV-Tert-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Tert(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_009354.2;EST2; TCS1; TP2; TR; TRT
Gene lenght: 3366
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Tert-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Tert-3×FLAG-CopGFP-Puro is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Tert-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga cccgcgctcc tcgttgcccc 2341 gcggtgcgct ctctgctgcg cagccgatac cgggaggtgt ggccgctggc aacctttgtg 2401 cggcgcctgg ggcccgaggg caggcggctt gtgcaacccg gggacccgaa gatctaccgc 2461 actttggttg cccaatgcct agtgtgcatg cactggggct cacagcctcc acctgccgac 2521 ctttccttcc accaggtgtc atccctgaaa gagctggtgg ccagggttgt gcagagactc 2581 tgcgagcgca acgagagaaa cgtgctggct tttggctttg agctgcttaa cgaggccaga 2641 ggcgggcctc ccatggcctt cactagtagc gtgcgtagct acttgcccaa cactgttatt 2701 gagaccctgc gtgtcagtgg tgcatggatg ctactgttga gccgagtggg cgacgacctg 2761 ctggtctacc tgctggcaca ctgtgctctt tatcttctgg tgccccccag ctgtgcctac 2821 caggtgtgtg ggtctcccct gtaccaaatt tgtgccacca cggatatctg gccctctgtg 2881 tccgctagtt acaggcccac ccgacccgtg ggcaggaatt tcactaacct taggttctta 2941 caacagatca agagcagtag tcgccaggaa gcaccgaaac ccctggcctt gccatctcga 3001 ggtacaaaga ggcatctgag tctcaccagt acaagtgtgc cttcagctaa gaaggccaga 3061 tgctatcctg tcccgagagt ggaggaggga ccccacaggc aggtgctacc aaccccatca 3121 ggcaaatcat gggtgccaag tcctgctcgg tcccccgagg tgcctactgc agagaaagat 3181 ttgtcttcta aaggaaaggt gtctgacctg agtctctctg ggtcggtgtg ctgtaaacac 3241 aagcccagct ccacatctct gctgtcacca ccccgccaaa atgcctttca gctcaggcca 3301 tttattgaga ccagacattt cctttactcc aggggagatg gccaagagcg tctaaacccc 3361 tcattcctac tcagcaacct ccagcctaac ttgactgggg ccaggagact ggtggagatc 3421 atctttctgg gctcaaggcc taggacatca ggaccactct gcaggacaca ccgtctatcg 3481 cgtcgatact ggcagatgcg gcccctgttc caacagctgc tggtgaacca tgcagagtgc 3541 caatatgtca gactcctcag gtcacattgc aggtttcgaa cagcaaacca acaggtgaca 3601 gatgccttga acaccagccc accgcacctc atggatttgc tccgcctgca cagcagtccc 3661 tggcaggtat atggttttct tcgggcctgt ctctgcaagg tggtgtctgc tagtctctgg 3721 ggtaccaggc acaatgagcg ccgcttcttt aagaacttaa agaagttcat ctcgttgggg 3781 aaatacggca agctatcact gcaggaactg atgtggaaga tgaaagtaga ggattgccac 3841 tggctccgca gcagcccggg gaaggaccgt gtccccgctg cagagcaccg tctgagggag 3901 aggatcctgg ctacgttcct gttctggctg atggacacat acgtggtaca gctgcttagg 3961 tcattctttt acatcacaga gagcacattc cagaagaaca ggctcttctt ctaccgtaag 4021 agtgtgtgga gcaagctgca gagcattgga gtcaggcaac accttgagag agtgcggcta 4081 cgggagctgt cacaagagga ggtcaggcat caccaggaca cctggctagc catgcccatc 4141 tgcagactgc gcttcatccc caagcccaac ggcctgcggc ccattgtgaa catgagttat 4201 agcatgggta ccagagcttt gggcagaagg aagcaggccc agcatttcac ccagcgtctc 4261 aagactctct tcagcatgct caactatgag cggacaaaac atcctcacct tatggggtct 4321 tctgtactgg gtatgaatga catctacagg acctggcggg cctttgtgct gcgtgtgcgt 4381 gctctggacc agacacccag gatgtacttt gttaaggcag atgtgaccgg ggcctatgat 4441 gccatccccc agggtaagct ggtggaggtt gttgccaata tgatcaggca ctcggagagc 4501 acgtactgta tccgccagta tgcagtggtc cggagagata gccaaggcca agtccacaag 4561 tcctttagga gacaggtcac caccctctct gacctccagc catacatggg ccagttcctt 4621 aagcatctgc aggattcaga tgccagtgca ctgaggaact ccgttgtcat cgagcagagc 4681 atctctatga atgagagcag cagcagcctg tttgacttct tcctgcactt cctgcgtcac 4741 agtgtcgtaa agattggtga caggtgctat acgcagtgcc agggcatccc ccagggctcc 4801 agcctatcca ccctgctctg cagtctgtgt ttcggagaca tggagaacaa gctgtttgct 4861 gaggtgcagc gggatgggtt gcttttacgt tttgttgatg actttctgtt ggtgacgcct 4921 cacttggacc aagcaaaaac cttcctcagc accctggtcc atggcgttcc tgagtatggg 4981 tgcatgataa acttgcagaa gacagtggtg aacttccctg tggagcctgg taccctgggt 5041 ggtgcagctc cataccagct gcctgctcac tgcctgtttc cctggtgtgg cttgctgctg 5101 gacactcaga ctttggaggt gttctgtgac tactcaggtt atgcccagac ctcaattaag 5161 acgagcctca ccttccagag tgtcttcaaa gctgggaaga ccatgcggaa caagctcctg 5221 tcggtcttgc ggttgaagtg tcacggtcta tttctagact tgcaggtgaa cagcctccag 5281 acagtctgca tcaatatata caagatcttc ctgcttcagg cctacaggtt ccatgcatgt 5341 gtgattcagc ttccctttga ccagcgtgtt aggaagaacc tcacattctt tctgggcatc 5401 atctccagcc aagcatcctg ctgctatgct atcctgaagg tcaagaatcc aggaatgaca 5461 ctaaaggcct ctggctcctt tcctcctgaa gccgcacatt ggctctgcta ccaggccttc 5521 ctgctcaagc tggctgctca ttctgtcatc tacaaatgtc tcctgggacc tctgaggaca 5581 gcccaaaaac tgctgtgccg gaagctccca gaggcgacaa tgaccatcct taaagctgca 5641 gctgacccag ccctaagcac agactttcag accattttgg acggatccGA CTACAAAGAC 5701 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGtagcgg 5761 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 5821 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 5881 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 5941 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 6001 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 6061 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 6121 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 6181 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 6241 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 6301 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 6361 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 6421 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 6481 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 6541 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 6601 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 6661 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 6721 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 6781 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 6841 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 6901 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 6961 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 7021 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 7081 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 7141 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 7201 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 7261 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 7321 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 7381 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 7441 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 7501 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 7561 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 7621 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 7681 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 7741 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 7801 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 7861 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 7921 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 7981 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 8041 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 8101 actgacaatt ccgtggtgtt gtcggggaaA TCATCGTCCT TTCCTtggct gctcgcctgt 8161 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 8221 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctTcgcctt 8281 cgccctcaga cgagtcggat ctccctttgg Gccgcctccc cgcctggtac ctttaagacc 8341 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 8401 agggctaatt cactcccaac gaaGataaga tctgcttttt gcttgtactg ggtctctctg 8461 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 8521 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 8581 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 8641 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 8701 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 8761 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 8821 ttatcatgtc tggctctagc taTCCCGCCC CTAACTCCGC CCAtcccgcc cctaactccg 8881 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 8941 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 9001 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 9061 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 9121 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 9181 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 9241 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 9301 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 9361 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 9421 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 9481 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 9541 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 9601 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 9661 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 9721 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 9781 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 9841 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 9901 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 9961 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 10021 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 10081 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 10141 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 10201 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 10261 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 10321 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 10381 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 10441 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 10501 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 10561 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 10621 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 10681 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 10741 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 10801 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 10861 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 10921 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 10981 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 11041 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 11101 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 11161 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 11221 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 11281 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 11341 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 11401 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 11461 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 11521 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 11581 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 11641 ggccagtgcc aagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.