pLV2-UbC-Cd63-Nluc-Zeo plasmid

pLV2-UbC-Cd63-Nluc-Zeo plasmid

PVT111535

pLV2-UbC-Cd63-Nluc-Zeo plasmid Information

Plasmid name: pLV2-UbC-Cd63(mouse)-Nluc-Zeo
Alias: NM_001282966.1;ME491; Tspan30
Gene lenght: 714
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Zeo
Fluorescent labeling:
Promoter: UbC
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: UbC-F1
Reverse primer:
Copy number: High
Induction:

pLV2-UbC-Cd63-Nluc-Zeo plasmid Description

pLV2-UbC-Cd63-Nluc-Zeo is a plasmid suitable for gene expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-UbC-Cd63-Nluc-Zeo plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg 2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc 2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca 2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg 2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac 2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa 2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat 2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc 2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccaccATG GCGGTGGAAG 2641 GAGGAATGAA GTGTGTCAAG TTTTTGCTCT ACGTTCTCCT GCTGGCCTTC TGCGCCTGTG 2701 CAGTGGGATT GATCGCCATT GGTGTAGCGG TTCAGGTTGT CTTGAAGCAG GCCATTACCC 2761 ATGAGACTAC TGCTGGCTCG CTGTTGCCTG TGGTCATCAT TGCAGTGGGT GCCTTCCTCT 2821 TCCTGGTGGC CTTTGTGGGC TGCTGTGGGG CCTGCAAGGA GAACTACTGT CTCATGATTA 2881 CATTTGCCAT CTTCCTGTCT CTTATCATGC TTGTGGAGGT GGCTGTGGCC ATTGCTGGCT 2941 ATGTGTTTAG AGACCAGGTG AAGTCAGAGT TTAATAAAAG CTTCCAGCAG CAGATGCAGA 3001 ATTACCTTAA AGACAACAAA ACAGCCACTA TTTTGGACAA ATTGCAGAAA GAAAATAACT 3061 GCTGTGGAGC TTCTAACTAC ACAGACTGGG AAAACATCCC CGGCATGGCC AAGGACAGAG 3121 TCCCCGATTC TTGCTGCATC AACATAACTG TGGGCTGTGG GAATGATTTC AAGGAATCCA 3181 CTATCCATAC CCAGGGCTGC GTGGAGACTA TAGCAATATG GCTAAGGAAG AACATACTGC 3241 TGGTGGCTGC AGCGGCCCTG GGCATTGCTT TTGTGGAGGT CTTGGGAATT ATCTTCTCCT 3301 GCTGTCTGGT GAAGAGTATT CGAAGTGGCT ATGAAGTAAT GGGCGGCGGC GGCTCCATGG 3361 TCTTCACACT CGAAGATTTC GTTGGGGACT GGCGACAGAC AGCCGGCTAC AACCTGGACC 3421 AAGTCCTTGA ACAGGGAGGT GTGTCCAGTT TGTTTCAGAA TCTCGGGGTG TCCGTAACTC 3481 CGATCCAAAG GATTGTCCTG AGCGGTGAAA ATGGGCTGAA GATCGACATC CATGTCATCA 3541 TCCCGTATGA AGGTCTGAGC GGCGACCAAA TGGGCCAGAT CGAAAAAATT TTTAAGGTGG 3601 TGTACCCTGT GGATGATCAT CACTTTAAGG TGATCCTGCA CTATGGCACA CTGGTAATCG 3661 ACGGGGTTAC GCCGAACATG ATCGACTATT TCGGACGGCC GTATGAAGGC ATCGCCGTGT 3721 TCGACGGCAA AAAGATCACT GTAACAGGGA CCCTGTGGAA CGGCAACAAA ATTATCGACG 3781 AGCGCCTGAT CAACCCCGAC GGCTCCCTGC TGTTCCGAGT AACCATCAAC GGAGTGACCG 3841 GCTGGCGGCT GTGCGAACGC ATTCTGGCGg agggcagagg aagtcttcta acatgcggtg 3901 acgtggagga gaatcccggc cctATGGCCA AGTTGACCAG TGCCGTTCCG GTGCTCACCG 3961 CGCGCGACGT CGCCGGAGCG GTCGAGTTCT GGACCGACCG GCTCGGGTTC TCCCGGGACT 4021 TCGTGGAGGA CGACTTCGCC GGTGTGGTCC GGGACGACGT GACCCTGTTC ATCAGCGCGG 4081 TCCAGGACCA GGTGGTGCCG GACAACACCC TGGCCTGGGT GTGGGTGCGC GGCCTGGACG 4141 AGCTGTACGC CGAGTGGTCG GAGGTCGTGT CCACGAACTT CCGGGACGCC TCCGGGCCGG 4201 CCATGACCGA GATCGGCGAG CAGCCGTGGG GGCGGGAGTT CGCCCTGCGC GACCCGGCCG 4261 GCAACTGCGT GCACTTCGTG GCCGAGGAGC AGGACTGAat cgatagatcc taatcaacct 4321 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 4381 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 4441 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 4501 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 4561 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 4621 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 4681 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt 4741 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 4801 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 4861 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa 4921 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 4981 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt 5041 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 5101 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 5161 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 5221 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc 5281 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc 5341 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 5401 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 5461 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 5521 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 5581 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 5641 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 5701 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 5761 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 5821 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 5881 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 5941 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 6001 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 6061 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 6121 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 6181 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 6241 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 6301 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 6361 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 6421 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 6481 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 6541 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 6601 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 6661 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 6721 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 6781 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 6841 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 6901 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 6961 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 7021 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 7081 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 7141 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 7201 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 7261 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 7321 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc 7381 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 7441 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg 7501 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag 7561 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga 7621 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca 7681 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 7741 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc 7801 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc 7861 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta 7921 gt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.