pLV2-TRE3GS-3×FLAG-EGFP-SCAP-T

pLV2-TRE3GS-3×FLAG-EGFP-SCAP-TetOne-Puro plasmid

PVT111178

pLV2-TRE3GS-3×FLAG-EGFP-SCAP-TetOne-Puro plasmid Information

Plasmid name: pLV2-TRE3GS-3×FLAG-EGFP-SCAP(human)-TetOne-Puro
Alias: NM_012235.4
Gene lenght: 3840
Host: Mammalian cells,lentivirus
Use(s): Gene expression,Tetracycline induction
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV2-TRE3GS-3×FLAG-EGFP-SCAP-TetOne-Puro plasmid Description

pLV2-TRE3GS-3×FLAG-EGFP-SCAP-TetOne-Puro Plasmid suitable for gene expression in mammalian cells, tetracycline-inducible, also suitable for lentivirus packaging.

pLV2-TRE3GS-3×FLAG-EGFP-SCAP-TetOne-Puro plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 4681 tactcggtca tggtcgcgtt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac 4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt 5041 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg 5101 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg 5161 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg 5221 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga 5281 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc 5341 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg 5401 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac 5461 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga 5521 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg 5581 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc 5641 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt 5701 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg 5761 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg 5821 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg 5881 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc 5941 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc 6001 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg 6061 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga 6121 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg 6181 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc 6241 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg 6301 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac 6361 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca 6421 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt 6481 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac 6541 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc 6601 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt 6661 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcgccac catggactac 6721 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag 6781 cttatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 6841 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 6901 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 6961 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg 7021 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 7081 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 7141 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 7201 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 7261 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 7321 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 7381 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 7441 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 7501 ggcggtggag gcagtggtgg aggcggtagc atgaccctga ctgaaaggct gcgtgagaag 7561 atatctcggg ccttctacaa ccatgggctc ctctgtgcat cctatcccat ccccatcatc 7621 ctcttcacag ggttctgcat cttagcctgc tgctacccac tgctgaaact ccccttgcca 7681 ggaacaggac ctgtggaatt caccacccct gtgaaggatt actcgccccc acctgtggac 7741 tctgaccgca aacaaggaga gcctactgag cagcctgagt ggtatgtggg tgccccggtg 7801 gcttatgtcc agcagatatt tgtgaagtcc tcagtgtttc cctggcacaa gaacctcctg 7861 gcagtagatg tatttcgttc acctttgtcc cgggcattcc aactggtgga ggagatccgg 7921 aaccacgtgc tgagagacag ctctgggatc aggagcttgg aggagttgtg tctgcaagtg 7981 accgacctgc tgccaggcct taggaagctc aggaacctac tccctgagca tggatgcctg 8041 ctgctgtccc ctgggaactt ctggcagaat gactgggaac gcttccatgc tgatcctgac 8101 atcattggga ccatccacca gcacgagcct aaaaccctgc agacttcagc cacactcaaa 8161 gacttgttat ttggtgttcc tgggaagtac agcggggtga gcctctacac caggaagagg 8221 atggtctcct acaccatcac cctggtcttc cagcactacc atgccaagtt cctgggcagc 8281 ctgcgtgccc gcctgatgct tctgcacccc agccccaact gcagccttcg ggcggagagc 8341 ctggtccacg tgcacttcaa ggaggagatt ggtgtcgctg agctcatccc ccttgtgacc 8401 acctacatca tcttgtttgc ctacatctac ttctccacgc ggaagatcga catggtcaag 8461 tccaagtggg ggctggccct ggctgccgtg gtcacagtgc tcagctcgct gctcatgtct 8521 gtgggactct gcacactctt cggcctgacg cccaccctca atggcggcga gattttcccc 8581 taccttgtgg tggttattgg gttagagaat gtgttggtgc tcaccaagtc tgtggtctca 8641 accccggtag acctggaggt gaagctgcgg atcgcccaag gcctaagcag cgagagctgg 8701 tccatcatga agaacatggc cacggagctg ggcatcatcc tcatcggcta cttcacccta 8761 gtgcccgcca tccaggagtt ctgtctcttt gctgtcgtgg ggctggtgtc tgacttcttc 8821 cttcagatgc tgtttttcac cactgtcctg tccattgaca ttcgccggat ggagctagca 8881 gacctgaaca agcgactgcc ccctgaggcc tgcctgccct cagccaagcc agtgggacag 8941 ccaacgcgct acgagcggca gctggctgtg aggccgtcca caccccacac catcacgttg 9001 cagccgtctt ccttccgaaa cctgcggctc cccaagaggc tgcgtgttgt ctacttcctg 9061 gcccgcaccc gcctggcaca gcgcctcatc atggctggca ccgttgtctg gattggcatc 9121 ctggtataca cagacccagc agggctgcgc aactacctcg ctgcccaggt gacggaacag 9181 agcccattgg gtgagggagc cctggctccc atgcccgtgc ctagtggcat gctgcccccc 9241 agccacccgg accctgcctt ctccatcttc ccacctgatg cccctaagct acctgagaac 9301 cagacgtcgc caggcgagtc acctgagcgt ggaggtccag cagaggttgt ccatgacagc 9361 ccagtcccag aggtaacctg ggggcctgag gatgaggaac tttggaggaa attgtccttc 9421 cgccactggc cgacgctctt cagctattac aacatcacac tggccaagag gtacatcagc 9481 ctgctgcccg tcatcccagt cacgctccgc ctgaacccga gggaggctct ggagggccgg 9541 caccctcagg acggccgcag tgcctggccc ccaccggggc ccatacctgc tgggcactgg 9601 gaagcaggac ccaagggccc aggtggggtg caggcccatg gagacgtcac gctgtacaag 9661 gtggcggcgc tgggcctggc caccggcatc gtcttggtgc tgctgctgct ctgcctctac 9721 cgcgtgctat gcccgcgcaa ctacgggcag ctgggtggtg ggcccgggcg gcggaggcgc 9781 ggggagctgc cctgcgacga ctacggctat gcgccacccg agacggagat cgtgccgctt 9841 gtgctgcgcg gccacctcat ggacatcgag tgcctggcca gcgacggcat gctgctggtg 9901 agctgctgcc tggcaggcca cgtctgcgtg tgggacgcgc agaccgggga ttgcctaacg 9961 cgcattccgc gcccaggcag gcagcgccgg gacagtggcg tgggcagcgg gcttgaggct 10021 caggagagct gggaacgact ttcagatggt gggaaggctg gtccagagga gcctggggac 10081 agccctcccc tgagacaccg cccccggggc cctccgccgc cttccctctt cggggaccag 10141 cctgacctca cctgcttaat tgacaccaac ttttcagcgc agcctcggtc ctcacagccc 10201 actcagcccg agccccggca ccgggcggtc tgtggccgct ctcgggactc cccaggctat 10261 gacttcagct gcctggtgca gcgggtgtac caggaggagg ggctggcggc cgtctgcaca 10321 ccagccctgc gcccaccctc gcctgggccg gtgctgtccc aggcccctga ggacgagggt 10381 ggctcccccg agaaaggctc cccttccctc gcctgggccc ccagtgccga gggttccatc 10441 tggagcttgg agctgcaggg caacctcatc gtggtggggc ggagcagcgg ccggctggag 10501 gtgtgggacg ccattgaagg ggtgctgtgc tgcagcagcg aggaggtctc ctcaggcatt 10561 accgctctgg tgttcttgga caaaaggatt gtggctgcac ggctcaacgg ttcccttgat 10621 ttcttctcct tggagaccca cactgccctc agccccctgc agtttagagg gaccccaggg 10681 cggggcagtt cccctgcctc tccagtgtac agcagcagcg acacagtggc ctgtcacctg 10741 acccacacag tgccctgtgc acaccaaaaa cccatcacag ccctgaaagc cgctgctggg 10801 cgcttggtga ctgggagcca agaccacaca ctgagagtgt tccgtctgga ggactcgtgc 10861 tgcctcttca cccttcaggg ccactcaggg gccatcacga ccgtgtacat tgaccagacc 10921 atggtgctgg ccagtggagg acaagatggg gccatctgcc tgtgggatgt actgactggc 10981 agccgggtca gccatgtgtt tgctcaccgt ggggatgtca cctcccttac ctgtaccacc 11041 tcctgtgtca tcagcagtgg cctggatgac ctcatcagca tctgggaccg cagcacaggc 11101 atcaagttct actccattca gcaggacctg ggctgtggtg caagcttggg tgtcatctca 11161 gacaacctgc tggtgactgg cggccagggc tgtgtctcct tttgggacct aaactacggg 11221 gacctgttac agacagtcta cctggggaag aacagtgagg cccagcctgc ccgccagatc 11281 ctggtgctgg acaacgctgc cattgtctgc aactttggca gtgagctcag cctggtgtat 11341 gtgccctctg tgctggagaa gctggactga ggatccagac cacctcccct gcgagctaag 11401 ctggacagcc aatgacgggt aagagagtga catttttcac taacctaaga caggagggcc 11461 gtcagagcta ctgcctaatc caaagacggg taaaagtgat aaaaatgtat cactccaacc 11521 taagacaggc gcagcttccg agggatttga gatccagaca tgataagata cattgatgag 11581 tttggacaaa ccaaaactag aatgcagtga aaaaaatgcc ttatttgtga aatttgtgat 11641 gctattgcct tatttgtaac cattataagc tgcaataaac aagttaagtc gataaactgg 11701 atctctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt gtttttgtag 11761 ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg cactgtaccc 11821 cccaatcccc ccttttcttt taaaggcgat accgtcgaga tccgttcact aatcgaatgg 11881 atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc 11941 tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca 12001 ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga 12061 ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt 12121 tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta 12181 aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt 12241 gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg 12301 tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc 12361 tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca 12421 gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca 12481 ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg 12541 ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca 12601 ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt 12661 tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg 12721 gccggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa 12781 agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt 12841 attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag 12901 tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag 12961 ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa 13021 ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc 13081 atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc 13141 ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg 13201 tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac 13261 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact 13321 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag 13381 agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa aagcagctgc 13441 ttatatgcag gatctgaggg ctcgccactc cccagtcccg cccaggccac gcctccctgg 13501 aaagtcccca gcggaaagtc ccttgtagca agctcgatat cagcagttct tgaagtactc 13561 cggatgcagc tctcgggcca cgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa 13621 cacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca agctggtgtt 13681 ctctccttta ttggcctctt ctaccttatc tggctcaact ggtactagct tgtagcacca 13741 tccaaaggtc agtggatatc tgacccctgg ccctggtgtg tagttctgct aatcagggaa 13801 gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcttt gggagtgaat 13861 tagcccttcc a

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.