pLKO.1-U6-CCN1-shRNA1-blast pl

pLKO.1-U6-CCN1-shRNA1-blast plasmid

PVT111159

pLKO.1-U6-CCN1-shRNA1-blast plasmid Information

Plasmid name: pLKO.1-U6-CCN1(human)-shRNA1-blast
Alias: CCACACGAGTTACCAATGA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene interference
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLKO.1-U6-CCN1-shRNA1-blast plasmid Description

pLKO.1-U6-CCN1-shRNA1-blast It is a plasmid suitable for gene interference in mammalian cells, and is also suitable for lentivirus packaging.

pLKO.1-U6-CCN1-shRNA1-blast plasmid Sequence

1 TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA 61 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG 121 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG 181 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC 241 ATGATTACGC CAAGCGCGCA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GAGCTGCAAG 301 CTTAATGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 361 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 421 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 481 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACATAA ACGGGTCTCT 541 CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA 601 GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC 661 TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTGGCGC 721 CCGAACAGGG ACTTGAAAGC GAAAGGGAAA CCAGAGGAGC TCTCTCGACG CAGGACTCGG 781 CTTGCTGAAG CGCGCACGGC AAGAGGCGAG GGGCGGCGAC TGGTGAGTAC GCCAAAAATT 841 TTGACTAGCG GAGGCTAGAA GGAGAGAGAT GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG 901 AGAATTAGAT CGCGATGGGA AAAAATTCGG TTAAGGCCAG GGGGAAAGAA AAAATATAAA 961 TTAAAACATA TAGTATGGGC AAGCAGGGAG CTAGAACGAT TCGCAGTTAA TCCTGGCCTG 1021 TTAGAAACAT CAGAAGGCTG TAGACAAATA CTGGGACAGC TACAACCATC CCTTCAGACA 1081 GGATCAGAAG AACTTAGATC ATTATATAAT ACAGTAGCAA CCCTCTATTG TGTGCATCAA 1141 AGGATAGAGA TAAAAGACAC CAAGGAAGCT TTAGACAAGA TAGAGGAAGA GCAAAACAAA 1201 AGTAAGACCA CCGCACAGCA AGCGGCCGCT GATCTTCAGA CCTGGAGGAG GAGATATGAG 1261 GGACAATTGG AGAAGTGAAT TATATAAATA TAAAGTAGTA AAAATTGAAC CATTAGGAGT 1321 AGCACCCACC AAGGCAAAGA GAAGAGTGGT GCAGAGAGAA AAAAGAGCAG TGGGAATAGG 1381 AGCTTTGTTC CTTGGGTTCT TGGGAGCAGC AGGAAGCACT ATGGGCGCAG CGTCAATGAC 1441 GCTGACGGTA CAGGCCAGAC AATTATTGTC TGGTATAGTG CAGCAGCAGA ACAATTTGCT 1501 GAGGGCTATT GAGGCGCAAC AGCATCTGTT GCAACTCACA GTCTGGGGCA TCAAGCAGCT 1561 CCAGGCAAGA ATCCTGGCTG TGGAAAGATA CCTAAAGGAT CAACAGCTCC TGGGGATTTG 1621 GGGTTGCTCT GGAAAACTCA TTTGCACCAC TGCTGTGCCT TGGAATGCTA GTTGGAGTAA 1681 TAAATCTCTG GAACAGATTT GGAATCACAC GACCTGGATG GAGTGGGACA GAGAAATTAA 1741 CAATTACACA AGCTTAATAC ACTCCTTAAT TGAAGAATCG CAAAACCAGC AAGAAAAGAA 1801 TGAACAAGAA TTATTGGAAT TAGATAAATG GGCAAGTTTG TGGAATTGGT TTAACATAAC 1861 AAATTGGCTG TGGTATATAA AATTATTCAT AATGATAGTA GGAGGCTTGG TAGGTTTAAG 1921 AATAGTTTTT GCTGTACTTT CTATAGTGAA TAGAGTTAGG CAGGGATATT CACCATTATC 1981 GTTTCAGACC CACCTCCCAA CCCCGAGGGG ACCCGACAGG CCCGAAGGAA TAGAAGAAGA 2041 AGGTGGAGAG AGAGACAGAG ACAGATCCAT TCGATTAGTG AACGGATCTC GACGGTATCG 2101 ATCACGAGAC TAGCCTCGAG CGGCCGCCCC CTTCACCGAG GGCCTATTTC CCATGATTCC 2161 TTCATATTTG CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA ATTTGACTGT 2221 AAACACAAAG ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT 2281 TGCAGTTTTA AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA 2341 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGGCC ACACGAGTTA 2401 CCAATGATTC AAGAGATCAT TGGTAACTCG TGTGGTTTTT TGAATTCTCG ACCTCGAGAC 2461 AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG GATTGGGGGG TACAGTGCAG 2521 GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC TAAAGAATTA CAAAAACAAA 2581 TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG CAGAGATCCA CTTTGGCCGC 2641 GGCTCGAGGG GGTTGGGGTT GCGCCTTTTC CAAGGCAGCC CTGGGTTTGC GCAGGGACGC 2701 GGCTGCTCTG GGCGTGGTTC CGGGAAACGC AGCGGCGCCG ACCCTGGGTC TCGCACATTC 2761 TTCACGTCCG TTCGCAGCGT CACCCGGATC TTCGCCGCTA CCCTTGTGGG CCCCCCGGCG 2821 ACGCTTCCTG CTCCGCCCCT AAGTCGGGAA GGTTCCTTGC GGTTCGCGGC GTGCCGGACG 2881 TGACAAACGG AAGCCGCACG TCTCACTAGT ACCCTCGCAG ACGGACAGCG CCAGGGAGCA 2941 ATGGCAGCGC GCCGACCGCG ATGGGCTGTG GCCAATAGCG GCTGCTCAGC AGGGCGCGCC 3001 GAGAGCAGCG GCCGGGAAGG GGCGGTGCGG GAGGCGGGGT GTGGGGCGGT AGTGTGGGCC 3061 CTGTTCCTGC CCGCGCGGTG TTCCGCATTC TGCAAGCCTC CGGAGCGCAC GTCGGCAGTC 3121 GGCTCCCTCG TTGACCGAAT CACCGACCTC TCTCCCCAGG GGGATCCACC ATGGCCAAGC 3181 CTTTGTCTCA AGAAGAATCC ACCCTCATTG AAAGAGCAAC GGCTACAATC AACAGCATCC 3241 CCATCTCTGA AGACTACAGC GTCGCCAGCG CAGCTCTCTC TAGCGACGGC CGCATCTTCA 3301 CTGGTGTCAA TGTATATCAT TTTACTGGGG GACCTTGTGC AGAACTCGTG GTGCTGGGCA 3361 CTGCTGCTGC TGCGGCAGCT GGCAACCTGA CTTGTATCGT CGCGATCGGA AATGAGAACA 3421 GGGGCATCTT GAGCCCCTGC GGACGGTGCC GACAGGTGCT TCTCGATCTG CATCCTGGGA 3481 TCAAAGCCAT AGTGAAGGAC AGTGATGGAC AGCCGACGGC AGTTGGGATT CGTGAATTGC 3541 TGCCCTCTGG TTATGTGTGG GAGGGCTAAG GTACCTTTAA GACCAATGAC TTACAAGGCA 3601 GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC 3661 CAACGAAGAC AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 3721 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 3781 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 3841 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT 3901 CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT GTTTATTGCA 3961 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT 4021 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGCTC 4081 TAGCTATCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT 4141 CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT 4201 CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGGACG TACCCAATTC 4261 GCCCTATAGT GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG 4321 GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG 4381 GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG 4441 CGAATGGGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 4501 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT 4561 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT 4621 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG 4681 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 4741 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 4801 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 4861 AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAGGTGG CACTTTTCGG 4921 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 4981 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT 5041 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT 5101 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG 5161 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 5221 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT 5281 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG 5341 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT 5401 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA 5461 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 5521 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA 5581 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG 5641 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC 5701 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT 5761 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 5821 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG 5881 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA 5941 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 6001 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 6061 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 6121 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 6181 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC 6241 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 6301 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 6361 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 6421 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC 6481 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 6541 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 6601 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 6661 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT 6721 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC 6781 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.