pCMV-MCS-Halo-Neo plasmid

pCMV-MCS-Halo-Neo plasmid

PVT111041

pCMV-MCS-Halo-Neo plasmid Information

Plasmid name: pCMV-MCS-Halo-Neo
Alias:
Gene lenght:
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-MCS-Halo-Neo plasmid Description

pCMV-MCS-Halo-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-MCS-Halo-Neo plasmid Sequence

1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat 781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc 841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa 901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac 961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta 1021 aggctagagt attaatacga ctcactatag ggctagcaaa gcgatcgctt ccgaattcct 1081 accgcggata tctagatttg ggcccaattc ctgcaggcga gctctcgagc caaccactga 1141 ggatctgtac tttcagagcg ataacgatgg atccgaaatc ggtactggct ttccattcga 1201 cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg gtccgcgcga 1261 tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat 1321 catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg gtatgggcaa 1381 atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca tggatgcctt 1441 catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg gctccgctct 1501 gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt 1561 catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga ccttccaggc 1621 cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg 1681 tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga 1741 gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat 1801 cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca 1861 gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga 1921 agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct 1981 gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc 2041 tactctggag atttccggtt aatagaattg gcatgcaagc tgatccggct gctaacaaag 2101 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg 2161 gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact 2221 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta 2281 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag 2341 gttcaggggg agatgtggga ggttttttta agcaagtaaa acctctacaa atgtggtaaa 2401 atcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg 2461 catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg cctgaaataa 2521 cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg 2581 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 2641 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 2701 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 2761 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 2821 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 2881 gcttttttgg aggcctaggc ttttgcaaaa agcttgattc ttctgacaca acagtctcga 2941 acttaaggct agagccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc 3001 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 3061 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 3121 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 3181 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 3241 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 3301 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 3361 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 3421 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 3481 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 3541 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 3601 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 3661 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 3721 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 3781 gaccaagcga cgcccaacct gccatcacga tggccgcaat aaaatatctt tattttcatt 3841 acatctgtgt gttggttttt tgtgtgaatc gatagcgata aggatcctct ttgcgcttgc 3901 gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg 3961 cggactggct ttctacgtaa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 4021 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 4081 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 4141 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 4201 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 4261 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 4321 aatgatgagc actttcaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 4381 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 4441 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 4501 aaccatgagt gataacactg cggccaactt acttctgaca actatcggag gaccgaagga 4561 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 4621 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 4681 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 4741 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 4801 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 4861 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 4921 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 4981 ttggtaattc gaaatgaccg accaagcgac gcccaaccgg tatcagctca ctcaaaggcg 5041 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 5101 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 5161 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 5221 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 5281 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 5341 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 5401 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 5461 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 5521 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 5581 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 5641 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 5701 cagcagatta cgcgcagaaa aaaaggattt caagaagatc ctttgatctt ttctacgggg 5761 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 5821 aggatcttca cctagatcct tttatagtcc ggaaatacag gaacgcacgc tggatggccc 5881 ttcgctggga tggtgaaacc atgaaaaatg gcagcttcag tggattaagt gggggtaatg 5941 tggcctgtac cctctggttg cataggtatt catacggtta aaatttatca ggcgcgattg 6001 cggcagtttt tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcgctga 6061 acgcgtttta gcggtgcgta caattaaggg attatggtaa atccacttac tgtctgccct 6121 cgtagccatc gagataaacc gcagtactcc ggccacgatg cgtccggcgt agaggatcga 6181 gatct

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.