pCMV-Ccr2-3×HA-mCMV-EGFP-Neo p

pCMV-Ccr2-3×HA-mCMV-EGFP-Neo plasmid

PVT111015

pCMV-Ccr2-3×HA-mCMV-EGFP-Neo plasmid Information

Plasmid name: pCMV-Ccr2(mouse-toxic-opt)-3×HA-mCMV-EGFP-Neo
Alias: NM_009915.2;Cc-ckr-2; Ccr2a; Ccr2b; Ckr2; Ckr2a; Ckr2b;Cmkbr2; mJe-r
Gene lenght: 1119
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Ccr2-3×HA-mCMV-EGFP-Neo plasmid Description

pCMV-Ccr2-3×HA-mCMV-EGFP-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Ccr2-3×HA-mCMV-EGFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCgc caccATGGAG GACAACAATA TGCTGCCCCA 661 GTTTATCCAC GGAATCCTGA GCACCTCTCA CAGCCTGTTC ACCAGGTCCA TTCAGGAGCT 721 GGACGAGGGC GCCACTACAC CTTACGATTA CGACGACGGC GAGCCTTGCC ACAAGACATC 781 CGTGAAGCAG ATCGGCGCAT GGATCCTGCC TCCTCTGTAT AGCCTGGTGT TCATCTTCGG 841 GTTCGTGGGG AACATGCTGG TGATCATCAT CCTGATCGGC TGTAAGAAGC TGAAGTCCAT 901 GACCGACATT TATCTGCTGA ACCTGGCCAT CTCAGACCTG CTGTTCCTGC TGACCCTGCC 961 TTTTTGGGCA CACTATGCTG CCAACGAGTG GGTGTTCGGG AATATCATGT GTAAGGTGTT 1021 TACAGGCCTG TATCACATCG GCTATTTCGG CGGCATCTTC TTTATCATCC TCCTGACAAT 1081 TGACAGATAT CTGGCAATTG TGCACGCCGT GTTTGCCCTG AAAGCACGCA CCGTGACATT 1141 CGGCGTGATT ACAAGCGTGG TGACTTGGGT CGTGGCCGTG TTTGCCAGCC TGCCAGGGAT 1201 CATCTTTACA AAGAGCAAGC AGGACGACCA CCACTATACA TGTGGCCCCT ACTTTACACA 1261 GCTGTGGAAG AACTTTCAGA CAATCATGAG GAACATCCTG TCTCTCATCC TGCCTCTGCT 1321 GGTGATGGTG ATCTGTTACT CCGGCATTCT GCACACCCTG TTTAGGTGTA GGAATGAGAA 1381 GAAAAGACAT AGAGCCGTGA GGCTGATCTT CGCTATCATG ATCGTGTACT TCCTGTTTTG 1441 GACCCCTTAC AACATCGTCC TGTTCCTGAC AACCTTCCAG GAGTCCCTGG GGATGTCCAA 1501 CTGTGTGATT GACAAGCATC TGGATCAGGC CATGCAGGTG ACCGAAACAC TGGGGATGAC 1561 CCACTGCTGC ATTAACCCAG TGATTTACGC CTTCGTGGGC GAGAAGTTTA GAAGGTACCT 1621 GTCTATCTTC TTCCGCAAGC ACATTGCCAA GAGGCTGTGC AAGCAGTGCC CCGTGTTTTA 1681 CAGAGAGACC GCAGACAGAG TTAGCTCCAC CTTTACTCCT AGCACCGGCG AGCAGGAGGT 1741 GTCCGTCGGA CTGAAGCTTT ACCCATACGA CGTACCTGAT TATGCAACTT ATCCTTATGA 1801 TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC TACGCATAGG ATCCGGGCCC 1861 GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC 1921 CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA 1981 AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG 2041 GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG 2101 GGCTCTATGG TCTAGAGATA TACTGAGTCA TTAGGGACTT TCCAATGGGT TTTGCCCAGT 2161 ACATAAGGTC AATAGGGGTG AATCAACAGG AAAGTCCCAT TGGAGCCAAG TACACTGAGT 2221 CAATAGGGAC TTTCCATTGG GTTTTGCCCA GTACAAAAGG TCAATAGGGG GTGAGTCAAT 2281 GGGTTTTTCC CATTATTGGC ACGTACATAA GGTCAATAGG GGTGAGTCAT TGGGTTTTTC 2341 CAGCCAaTTT AATTAAAACG CCATGTACTT TCCCACCATT GACGTCAATG GGCTATTGAA 2401 ACTAATGCAA CGTGACCTTT AAACGGTACT TTCCCATAGC TGATTAATGG GAAAGTACCG 2461 TTCTCGAGCC AATACACGTC AATGGGAAGT GAAAGGGCAG CCAAAACGTA ACACCGCCCC 2521 GGTTTTCCCC TGGAAATTCC ATATTGGCAC GCATTCTATT GGCTGAGCTG CGTTCTACGT 2581 GGGTATAAGA GGCGCGACCA GCGTCGGTAC CGTCGCAGTC TTCGGTCTGA CCACCGTAGA 2641 ACGCAGATCa ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 2701 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 2761 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 2821 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 2881 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 2941 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 3001 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 3061 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 3121 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 3181 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 3241 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 3301 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 3361 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca 3421 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 3481 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 3541 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 3601 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa 3661 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 3721 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 3781 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 3841 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 3901 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 3961 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 4021 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 4081 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 4141 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 4201 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta 4261 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4321 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4381 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 4441 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 4501 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 4561 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga 4621 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4681 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4741 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 4801 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 4861 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 4921 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 4981 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 5041 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 5101 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 5161 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 5221 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 5281 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 5341 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5401 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 5461 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 5521 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 5581 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 5641 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 5701 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 5761 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 5821 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt 5881 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 5941 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 6001 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 6061 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 6121 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 6181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 6241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 6301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 6361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 6421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 6481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 6541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 6601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 6661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.