pLV3-CMV-FLAG-PTPRS-APEX2-HA-P

pLV3-CMV-FLAG-PTPRS-APEX2-HA-Puro plasmid

PVT111014

pLV3-CMV-FLAG-PTPRS-APEX2-HA-Puro plasmid Information

Plasmid name: pLV3-CMV-FLAG-PTPRS(human)-APEX2-HA-Puro
Alias: NM_002850.4;PTP-sigma; PTPSIGMA; R-PTP-S; R-PTP-sigma
Gene lenght: 5844
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FLAG-PTPRS-APEX2-HA-Puro plasmid Description

pLV3-CMV-FLAG-PTPRS-APEX2-HA-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-FLAG-PTPRS-APEX2-HA-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCgccac cgatggcgcc cacctggggc cctggcatgg tgtctgtggt 2341 tggtcccatg ggcctccttg tggtcctgct cgttggaggc tgtgcagcag actacaagga 2401 tgacgacgat aagGGTGGTG GTGGTAGCga agagcccccc aggtttatca aagaacccaa 2461 ggaccagatc ggcgtgtcgg ggggtgtggc ctctttcgtg tgtcaggcca cgggtgaccc 2521 caagccacga gtgacctgga acaagaaggg caagaaggtc aactctcagc gctttgagac 2581 gattgagttt gatgagagtg caggggcagt gctgaggatc cagccgctga ggacaccgcg 2641 ggatgaaaac gtgtacgagt gtgtggccca gaactcggtt ggggagatca cagtccatgc 2701 caagcttact gtcctccgag aggaccagct gccctctggc ttccccaaca tcgacatggg 2761 cccacagttg aaggtggtgg agcggacacg gacagccacc atgctctgtg cagccagcgg 2821 caaccctgac cctgagatca cctggttcaa ggacttcctg cctgtggatc ctagtgccag 2881 caatggacgc atcaaacagc tgcgatcaga aacctttgaa agcactccga ttcgaggagc 2941 cctgcagatt gaaagcagtg aggaaaccga ccagggcaaa tatgagtgtg tggccaccaa 3001 cagcgccggc gtgcgctact cctcacctgc caacctctac gtgcgagagc ttcgagaagt 3061 ccgccgcgtg gccccgcgct tctccatcct gcccatgagc cacgagatca tgccaggggg 3121 caacgtgaac atcacctgcg tggccgtggg ctcgcccatg ccatacgtga agtggatgca 3181 gggggccgag gacctgaccc ccgaggatga catgcccgtg ggtcggaacg tgctggaact 3241 cacagatgtc aaggactcgg ccaactacac ctgcgtggcc atgtccagcc tgggcgtcat 3301 tgaggcggtt gctcagatca cggtgaaatc tctccccaaa gctcccggga ctcccatggt 3361 gactgagaac acagccacca gcatcaccat cacgtgggac tcgggcaacc cagatcctgt 3421 gtcctattac gtcatcgaat ataaatccaa gagccaagac gggccgtatc agattaaaga 3481 ggacatcacc accacacgtt acagcatcgg cggcctgagc cccaactcgg agtacgagat 3541 ctgggtgtcg gccgtcaact ccatcggcca ggggcccccc agcgagtccg tggtcacccg 3601 cacaggcgag caggccccgg ccagcgcgcc gcggaacgtg caagcccgga tgctcagcgc 3661 gaccaccatg attgtgcagt gggaggagcc ggtggagccc aacggcctga tccgcggcta 3721 ccgcgtctac tacaccatgg aaccggagca ccccgtgggc aactggcaga agcacaacgt 3781 ggacgacagc ctgctgacca ccgtgggcag cctgctggag gacgagacct acaccgtgcg 3841 ggtgctcgcc ttcacctccg tcggcgacgg gcccctctcg gaccccatcc aggtcaagac 3901 gcagcaggga gtgccgggcc agcccatgaa cctgcgggcc gaggccaggt cggagaccag 3961 catcacgctg tcctggagcc ccccgcggca ggagagtatc atcaagtacg agctcctctt 4021 ccgggaaggc gaccatggcc gggaggtggg aaggaccttc gacccgacga cttcctacgt 4081 ggtggaggac ctgaagccca acacggagta cgccttccgc ctggcggccc gctcgccgca 4141 gggcctgggc gccttcaccc ccgtggtgcg gcagcgcacg ctgcagtcca aaccgtcagc 4201 cccccctcaa gacgttaaat gtgtcagcgt gcgctccacg gccattttgg taagttggcg 4261 cccgccgccg ccggaaacgc acaacggggc cctggtgggc tacagcgtcc gctaccgacc 4321 gctgggctca gaggacccgg aacccaagga ggtgaacggc atccccccga ccaccactca 4381 gatcctgctg gaggccttgg agaagtggac ccagtaccgc atcacgactg tcgctcacac 4441 agaggtggga ccagggcccg agagctcgcc cgtggtcgtc cgcaccgacg aggatgtgcc 4501 cagcgcgccg ccgcggaagg tggaggcgga ggcgctcaac gccacggcca tccgcgtgct 4561 gtggcgctcg cccgcgcccg gccggcagca cggccagatc cgcggctacc aggtccacta 4621 cgtgcgcatg gagggcgccg aggcccgcgg gccgccgcgc atcaaggacg tcatgctggc 4681 cgatgcccag tgggagacgg atgacacggc cgaatatgag atggtcatca caaacttgca 4741 gcctgagacc gcgtactcca tcacggtagc cgcctacacc atgaagggcg atggcgctcg 4801 cagcaaaccc aaggtggttg tgaccaaggg agcagtgctg ggccgcccaa ccctgtcggt 4861 gcagcagacc cccgagggca gcctgctggc acgctgggag cccccggctg gcaccgcgga 4921 ggaccaggtg ctgggctacc gcctgcagtt tggccgtgag gactcgacgc ccctggccac 4981 cctggagttc ccgccctccg aggaccgcta cacggcatca ggcgtgcaca agggggccac 5041 gtatgtgttc cggcttgcgg cccggagccg cggcggcctg ggcgaggagg cagccgaggt 5101 cctgagcatc ccggaggaca cgccccgtgg ccacccgcag attctggagg cggccggcaa 5161 cgcctcggcc gggaccgtcc ttctccgctg gctgccaccc gtgcccgccg agcgcaacgg 5221 ggccatcgtc aaatacacgg tggccgtgcg ggaggccggt gccctgggcc ctgcccgaga 5281 gactgagctg ccggcagcgg ctgagccggg cgcggagaac gcgctcacgc tgcagggcct 5341 gaagcccgac acggcctatg acctccaagt gcgagcccac acgcgccggg gccctggccc 5401 cttcagcccc cccgtccgct accggacgtt cctgcgggac caagtctcgc ccaagaactt 5461 caaggtgaaa atgatcatga agacatcagt tctgctcagc tgggagttcc ctgacaacta 5521 caactcaccc acaccctaca agatccagta caatgggctc acactggatg tggatggccg 5581 taccaccaag aagctcatca cgcacctcaa gccccacacc ttctacaact ttgtgctgac 5641 caatcgcggc agcagcctgg gcggcctcca gcagacggtc accgcctgga ctgccttcaa 5701 cctgctcaac ggcaagccca gcgtcgcccc caagcctgat gctgacggct tcatcatggt 5761 gtatcttcct gacggccaga gccccgtgcc tgtccagagc tatttcattg tgatggtgcc 5821 actgcgcaag tctcgtggag gccaattcct gaccccgctg ggtagcccag aggacatgga 5881 tctggaagag ctcatccagg acatctcacg gctacagagg cgcagcctgc ggcactcgcg 5941 tcagctggag gtgccccggc cctatattgc agctcgcttc tctgtgctgc cacccacgtt 6001 ccatcccggc gaccagaagc agtatggcgg cttcgataac cggggcctgg agcccggcca 6061 ccgctatgtc ctcttcgtgc ttgccgtgct tcagaagagc gagcctacct ttgcagccag 6121 tcccttctca gaccccttcc agctggataa cccggacccc cagcccatcg tggatggcga 6181 ggaggggctt atctgggtga tcgggcctgt gctggccgtg gtcttcataa tctgcattgt 6241 cattgctatc ctgctctaca agaacaaacc cgacagtaaa cgcaaggact cagaaccccg 6301 caccaaatgc ctcctgaaca atgccgacct cgcccctcac caccccaagg accctgtgga 6361 aatgagacgc attaacttcc agactccaga ttcaggcctc aggagccccc tcagggagcc 6421 ggggtttcac tttgaaagca tgcttagcca cccgccaatt cccatcgcag acatggcgga 6481 gcacacggag cggctcaagg ccaacgacag cctcaagctc tcccaggagt atgagtccat 6541 cgaccctgga cagcagttca catgggaaca ttccaacctg gaagtgaaca agccgaagaa 6601 ccgctatgcc aacgtcatcg cctatgacca ctcccgtgtc atcctccagc ccattgaagg 6661 catcatgggc agtgattaca tcaatgccaa ctacgtggac ggctaccggt gtcagaacgc 6721 gtacattgcc acgcaggggc cgctgcctga gacctttggg gacttctggc gtatggtgtg 6781 ggagcagcgg tcggcgacca tcgtcatgat gacgcggctg gaggagaagt cacggatcaa 6841 gtgtgatcag tattggccca acagaggcac ggagacctac ggcttcatcc aggtcacgtt 6901 gctagatacc atcgagctgg ccacattctg cgtcaggaca ttctctctgc acaagaatgg 6961 ctccagtgag aaacgcgagg tccgccagtt ccagtttacg gcgtggccgg accatggcgt 7021 gcccgaatac ccaacgccct tcctggcttt cctgcggaga gtcaagacct gcaacccgcc 7081 agatgccggc cccatcgtgg ttcactgcag tgccggtgtg ggccgcacag gctgctttat 7141 cgtcatcgac gccatgcttg agcggatcaa gccagagaag acagtcgatg tctatggcca 7201 cgtgacgctc atgaggtccc agcgcaacta catggtgcag acggaggacc agtacagctt 7261 catccacgag gccctgctgg aggccgtggg ctgtggcaac acagaagtgc ccgcacgcag 7321 cctctatgcc tacatccaga agctggccca ggtggagcct ggcgaacacg tcactggcat 7381 ggaactcgag ttcaagcggc tggctaactc caaggcccac acgtcacgct tcatcagtgc 7441 caatctgcct tgtaacaagt tcaagaaccg cctggtgaac atcatgccct atgagagcac 7501 acgggtctgt ctgcaaccca tccggggtgt ggagggctct gactacatca acgccagctt 7561 cattgatggc tacaggcagc agaaggccta catcgcgaca caggggccgc tggcggagac 7621 cacggaagac ttctggcgca tgctgtggga gaacaattcg acgatcgtgg tgatgctgac 7681 caagctgcgg gagatgggcc gggagaagtg tcaccagtac tggccggccg agcgctctgc 7741 ccgctaccag tactttgtgg tagatccgat ggcagaatac aacatgcctc agtatatcct 7801 gcgagagttc aaggtcacag atgcccggga tggccagtcc cggactgtcc ggcagttcca 7861 gttcacagac tggccggaac agggtgtgcc aaagtcgggg gagggcttca tcgacttcat 7921 tggccaagtg cataagacta aggagcagtt tggccaggac ggccccatct ctgtccactg 7981 cagtgccggc gtgggcagga cgggcgtctt catcacgctt agcatcgtgc tggagcggat 8041 gcggtatgaa ggcgtggtgg acatctttca gacggtgaag atgctacgaa cccagcggcc 8101 ggccatggtg cagacagagg atgagtacca gttctgttac caggcggcac tggagtacct 8161 cggaagcttt gaccactatg caaccGGTGG TGGTGGTAGC GGTGGTGGTG GTAGCGGTGG 8221 TGGTGGTAGC ggaaagtctt acccaactgt gagtgctgat taccaggacg ccgttgagaa 8281 ggcgaagaag aagctcagag gcttcatcgc tgagaagaga tgcgctcctc taatgctccg 8341 tttggcattc cactctgctg gaacctttga caagggcacg aagaccggtg gacccttcgg 8401 aaccatcaag caccctgccg aactggctca cagcgctaac aacggtcttg acatcgctgt 8461 taggcttttg gagccactca aggcggagtt ccctattttg agctacgccg atttctacca 8521 gttggctggc gttgttgccg ttgaggtcac gggtggacct aaggttccat tccaccctgg 8581 aagagaggac aagcctgagc caccaccaga gggtcgcttg cccgatccca ctaagggttc 8641 tgaccatttg agagatgtgt ttggcaaagc tatggggctt actgaccaag atatcgttgc 8701 tctatctggg ggtcacacta ttggagctgc acacaaggag cgttctggat ttgagggtcc 8761 ctggacctct aatcctctta ttttcgacaa ctcatacttc acggagttgt tgagtggtga 8821 gaaggaaggt ctccttcagc taccttctga caaggctctt ttgtctgacc ctgtattccg 8881 ccctctcgtt gacaaatatg cagcggacga agatgccttc tttgctgatt acgctgaggc 8941 tcaccaaaag ctttccgagc ttgggtttgc tgatgcctac ccttacgatg taccggatta 9001 cgcataataa GGATCCGCGG CCGCGAAGGA TCTGCGATCG CTCCGGTGCC CGTCAGTGGG 9061 CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACGG 9121 GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC 9181 TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT 9241 TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC ATCTCTCCTT 9301 CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC GTTCTGCCGC 9361 CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA AAGCTCAGGT 9421 CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC CGGCTCTCCA 9481 CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT GTTCTGCGCC 9541 GTTACAGATC CAAGCTGTGA CCGGCGCCTA CGCTAGATGA CCGAGTACAA GCCCACGGTG 9601 CGCCTCGCCA CCCGCGACGA CGTCCCCCGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC 9661 GACTACCCCG CCACGCGCCA CACCGTCGAC CCGGACCGCC ACATCGAGCG GGTCACCGAG 9721 CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC 9781 GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC 9841 GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG 9901 ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC 9961 GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG 10021 GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC 10081 CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG 10141 CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGAGTCG ACAATCAACC TCTGGATTAC 10201 AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA 10261 TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC 10321 TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA 10381 CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC 10441 ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC 10501 ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC 10561 GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG 10621 ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT 10681 TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG 10741 AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGTACCT TTAAGACCAA TGACTTACAA 10801 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA 10861 CTCCCAACGA AAATAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT 10921 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT 10981 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC 11041 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT AGTAGTTCAT GTCATCTTAT 11101 TATTCAGTAT TTATAACTTG CAAAGAAATG AATATCAGAG AGTGAGAGGA ACTTGTTTAT 11161 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT 11221 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG 11281 GCTCTAGCTA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 11341 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 11401 TAGTGAGGAG GCTTTTTTGG AGGCCTAGAC TTTTGCAGAG ACGGCCCAAA TTCGTAATCA 11461 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 11521 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 11581 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA 11641 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC 11701 ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG 11761 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 11821 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 11881 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 11941 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC 12001 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 12061 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 12121 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 12181 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 12241 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT 12301 AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 12361 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 12421 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG 12481 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 12541 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 12601 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 12661 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 12721 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG 12781 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 12841 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 12901 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 12961 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 13021 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 13081 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 13141 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 13201 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 13261 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 13321 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 13381 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 13441 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 13501 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 13561 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC 13621 TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT 13681 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG 13741 GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG 13801 GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA 13861 GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG 13921 CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG 13981 CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA 14041 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG CCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.