pCMV-Sh3tc1-3×FLAG-Neo plasmid

pCMV-Sh3tc1-3×FLAG-Neo plasmid

PVT110874

pCMV-Sh3tc1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Sh3tc1(mouse)-3×FLAG-Neo
Alias: NM_194344.2;4631428G15
Gene lenght: 4038
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Sh3tc1-3×FLAG-Neo plasmid Description

pCMV-Sh3tc1-3×FLAG-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Sh3tc1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatgag gagcttcagg gaggaggagg 781 aggaggcgac ttctgaggag ctgttcacca tggggcgggg ccctgaggag cctcggggaa 841 gctgtagccc tgaagaccag attcagagtg tatccatggg cttgcccact ggccaggaga 901 gaacagggtc tgcggaggct gaggcaacag tgagaggtga tgtggctcct ccccgcatcg 961 tggtcgggct ggctgctgaa ccccctccca gtcaaatgac agtgtatcca acagacctga 1021 ccctgcaact gttggctgtg aggcggaaga gtggactgcg gagccccagc ctgcagcagg 1081 ccctgcgcag ccggctccgc ctgctggaga acgatagccg ggaagtggcc cgtgtgcttg 1141 gagaactgtc agccaggctg ctgtctatcc acagtgacca ggaccggatc gtggtgacat 1201 ttaagacttt tgaagaaatc tggaagtttt ctacctatca tgctcttggc ttcacacacc 1261 actgcctgga gaacctgctg gtagaccaga ccttctggct gctttccccc agtgaggagg 1321 aggagactgc catcctcatg tatgtaaaca aggatgcctt gaagcagaca cacaagaacc 1381 tcctcacaca ggaagggtcc atctttgttc tgtgtcctga ccaccgagta agaaggacga 1441 taggacccaa aggtgcaggg aagggtcccc agaccctcag acgggcttcg ggagtccctc 1501 tgggagtggc aatcctggaa acagactcct catcacccac acccaacatg tcctccaatg 1561 tggaagcagc agaggccgaa ccagagccct tgactccatt tcaccagtgg actctcaggg 1621 tcccatggga tcccatcgat gaatccatga gtggacctgg gacacctgat gcccagctca 1681 tgaggtttgg cctggcctct gcgatagctg acttccaggg ctcaggcccc gaagagatgt 1741 ccttcagcgt tggtgatgtc atcgagatcc tcggtgccca ggtgcctggc ctgccctggt 1801 gtgtgggccg gcacatggcc tctggccagg tgggctttgt gaggactggc ctcgtcagca 1861 tgcagggccc ggcatctgac ctggaaagtg ccattttcct cagtgaggaa gaaaggtcat 1921 tcttcagaag tgagggccac ttctcagacg aagatgcccg gcggctcctg agcaggacat 1981 ctggcacaga tgtgtgcacc acatgcagcc tggactggtt agaagaagct gagggcgaac 2041 agctggagca acgagaaatg tctctgcctc acctgaaccc agaaccacat gggactctgc 2101 agatggtaaa gaacatttta gaaaagtgca aggcctgccc tgaccacccc gaagccccgg 2161 tgtcctggag ccttggaggg gtgtccagaa gagcgagttc actggacctt gaggagcccc 2221 gcttctgcct ggatcctgag gatgactgga cagaccccga gcctctggac tcactgctgc 2281 aggtcctgaa tgcccctggt tatggggccc acttccggag cctgtacgat atctcacttc 2341 cctggctgag caccgcactc tatggctttg atgatgaaga agagctggct gaacgattgg 2401 cacaggcccg aggagttgcc aagaaagtcg acttgagcat ggccctggca aggctctgcc 2461 tcctgctggg gcgtctgtgt gcccgaaagc tcaaactatc ccaggcccgg gtgtactttg 2521 aagaggcact gggagctctg gaggggagtt ttggggacct ctccctggtg gcggcagtgt 2581 atagcagcct gactactgtg tacctaaagc agaagaacgc agagaagtgt gtgcaggtgg 2641 cacccaaagc agcggcactg ctcctgggga cacccggcca ctcctgcaac gcagatgcgg 2701 agctgctgaa gtatgcgctg cgcagggtca tctgtggcct cagcccacag gccgaggccc 2761 gggcctgctt cctgctggcc aggcactaca cccacctcaa acagcctgag gaggccctgc 2821 catacctgga gagactgctt cgcctcaaca gggatgcagg gagcccacag gcctcatggc 2881 ctgaagactg ttacctgctt ctggctgaca tttatggccg gaaatgcctg ccccatctgg 2941 cgttgagctg cctcagagtg tcctcactgt ggacaaggtg ctctctgcct ggctccttga 3001 ggagtgtgga cctggtactc cagaatgttc ctgggccaaa cagccaaagg agggcaggtc 3061 atagccttcc ctcccagatt gcttactacc tcagacaggc attggcctct ctggctcctg 3121 gcacaggaca ggcactgcgt gggccgctct atgccagcct ggctcaactg tacagtcacc 3181 accagcagta cagccaggcc attgccttca tgtctcaggc tgcagaagct gacactgcag 3241 ctggggtcca ccctgtcgtg gaccgcttgg tggccctcgc ctggttgcac atgctttgtg 3301 ggaacagctt agtagccatg gacatcctga agtgcatctc agatgcagcc gtggccaata 3361 aggaccagga gtgtgttatg atgaacatgg tggccatggc cctgaagagg atgggcagga 3421 cccgacaggc tgctgaaggc tacttccgag ctctgcacat ggcctatgca cagggccacc 3481 tgcagagcca ggcagtggtc ctggctaact ttggggccct gtgtctgcaa gctggtgcac 3541 gcagcttggc ccagcactac ctgcgcgagg ctgtggggct cttctcccag gtgcccagtg 3601 gcgtgtgtgg ccgggacttc acccaggtgc tcctgtggtt gggccaactg tgcacacgcc 3661 gggccctccc gcagcaggct aagtgctact acgagtgggc ctttctggtg gccgtggaga 3721 cagaccatct ggagagtcag ttacaggctg tgcagaagct gtgcctcttc tacagcaagg 3781 tgatgcccaa tgaggtgcga tgtgtcatct accacgagtt ccaactggca ctggcacgca 3841 agaccgccaa caaggtgctg gagggacagc tgctggaggc catcagccag ctctacctgt 3901 cgctgggcac tgagcgggcc tacaagtcgg ctctggatta cactaaacgg agcctgggga 3961 ttttcatcga cctgcagcag aaggagaagg aggcacgcgc ctggctgcag gcagggaaga 4021 tctactatat cctgcgtcag aatgagctgg tggacttata catccaggtg gcacagaatg 4081 cagccctgta tactggggac cccaagctgg gcctggaact gtttgaggca gcaggggaca 4141 ttttcttcaa tgggacctgg gagcgggaga aagctgtgtc cttctaccgg gaccgggcac 4201 tgcccctggc tgtgactgta ggagaccagg aagtggaact gcgcctgtgc aacaagctgg 4261 tggcccttct gtccgcgctg gaggcacccc aggagggcct ggagtttgcc catgaggcct 4321 tggcactcag catcacccta ggtgaccgcc tgaatgagcg agtggcctac cacaggctag 4381 ccaccctgca ccaccggctg ggccacgggg aactggctga gcacttcttc ctcaaggccc 4441 tgtcactctg cagctcgccc ctggaattcg atgaggagac cctgtactat gtgaaggtct 4501 acctggtgct gggtgacatc atcttctatg acctgaagga cccgtttgat gcagctgggt 4561 attaccagct ggcactggca gcagccgtgg acctgggcca caagaaagcc cagctgaaga 4621 tctacacccg cctggctacc atctaccacc acttcctcgt ggaccgtgag atgtccctct 4681 tcttctacca gaaagcaagg acctttgcct cagaactgaa cctccgcagg accaacctgg 4741 tgccccagcg cttctgtggc cgggcaccct ggctggcccc tggccaccca tccGGATCCG 4801 ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG 4861 ACAAGTAGTG AGGGCCCGAT ATCTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG 4921 ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT 4981 GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC 5041 CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG 5101 CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT 5161 TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT 5221 TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG 5281 GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC 5341 GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT 5401 GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT 5461 TTGGGCCGCC TCCCCGCCTG GAAACGGGGG AGGCTAACTG AAACACGGAA GGAGACAATA 5521 CCGGAAGGAA CCCGCGCTAT GACGGCAATA AAAAGACAGA ATAAAACGCA CGGGTGTTGG 5581 GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC 5641 GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG 5701 TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCAGA 5761 TCTGCGCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC 5821 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC 5881 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT 5941 AAATCGGGGC ATCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA 6001 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC 6061 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT 6121 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGGGGATTT CGGCCTATTG 6181 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT 6241 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 6301 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 6361 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 6421 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 6481 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 6541 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 6601 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 6661 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 6721 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 6781 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 6841 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 6901 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 6961 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 7021 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 7081 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 7141 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 7201 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 7261 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 7321 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 7381 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 7441 TCGCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC 7501 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 7561 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA 7621 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 7681 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC 7741 GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 7801 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 7861 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 7921 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 7981 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 8041 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 8101 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 8161 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 8221 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 8281 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 8341 CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 8401 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 8461 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 8521 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 8581 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 8641 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 8701 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 8761 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 8821 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 8881 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 8941 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 9001 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 9061 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 9121 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 9181 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 9241 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 9301 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 9361 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 9421 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 9481 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 9541 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 9601 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 9661 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 9721 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 9781 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 9841 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 9901 ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA GATCTCCCGA TCCCCTATGG 9961 TCGACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATCT GCTCCCTGCT 10021 TGTGTGTTGG AGGTCGCTGA GTAGTGCGCG AGCAAAATTT AAGCTACAAC AAGGCAAGGC 10081 TTGACCGACA ATTGCATGAA GAATCTGCTT AGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.