pLV2-CMV-ATIC-HA-Puro plasmid

pLV2-CMV-ATIC-HA-Puro plasmid

PVT110782

pLV2-CMV-ATIC-HA-Puro plasmid Information

Plasmid name: pLV2-CMV-ATIC(human)-HA-Puro
Alias: NM_004044.7;AICAR; AICARFT; HEL-S-70p; IMPCHASE; PURH
Gene lenght: 1776
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-ATIC-HA-Puro plasmid Description

pLV2-CMV-ATIC-HA-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV2-CMV-ATIC-HA-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggctcc cggccagctc gccttattta gtgtctctga 2881 caaaaccggc cttgtggaat ttgcaagaaa cctgaccgct cttggtttga atctggtcgc 2941 ttccggaggg actgcaaaag ctctcaggga tgctggtctg gcagtcagag atgtctctga 3001 gttgacggga tttcctgaaa tgttgggggg acgtgtgaaa actttgcatc ctgcagtcca 3061 tgctggaatc ctagctcgta atattccaga agataatgct gacatggcca gacttgattt 3121 caatcttata agagttgttg cctgcaatct ctatcccttt gtaaagacag tggcttctcc 3181 aggtgtaact gttgaggagg ctgtggagca aattgacatt ggtggagtaa ccttactgag 3241 agctgcagcc aaaaaccacg ctcgagtgac agtggtgtgt gaaccagagg actatgtggt 3301 ggtgtccacg gagatgcaga gctccgagag taaggacacc tccttggaga ctagacgcca 3361 gttagccttg aaggcattca ctcatacggc acaatatgat gaagcaattt cagattattt 3421 caggaaacag tacagcaaag gcgtatctca gatgcccttg agatatggaa tgaacccaca 3481 tcagacccct gcccagctgt acacactgca gcccaagctt cccatcacag ttctaaatgg 3541 agcccctgga tttataaact tgtgcgatgc tttgaacgcc tggcagctgg tgaaggaact 3601 caaggaggct ttaggtattc cagccgctgc ctctttcaaa catgtcagcc cagcaggtgc 3661 tgctgttgga attccactca gtgaagatga ggccaaagtc tgcatggttt atgatctcta 3721 taaaaccctc acacccatct cagcggcata tgcaagagca agaggggctg ataggatgtc 3781 ttcatttggt gattttgttg cattgtccga tgtttgtgat gtaccaactg caaaaattat 3841 ttccagagaa gtatctgatg gtataattgc cccaggatat gaagaagaag ccttgacaat 3901 actttccaaa aagaaaaatg gaaactattg tgtccttcag atggaccaat cttacaaacc 3961 agatgaaaat gaagttcgaa ctctctttgg tcttcattta agccagaaga gaaataatgg 4021 tgtcgtcgac aagtcattat ttagcaatgt tgttaccaaa aataaagatt tgccagagtc 4081 tgccctccga gacctcatcg tagccaccat tgctgtcaag tacactcagt ctaactctgt 4141 gtgctacgcc aagaacgggc aggttatcgg cattggagca ggacagcagt ctcgtataca 4201 ctgcactcgc cttgcaggag ataaggcaaa ctattggtgg cttagacacc atccacaagt 4261 gctttcgatg aagtttaaaa caggagtgaa gagagcagaa atctccaatg ccatcgatca 4321 atatgtgact ggaaccattg gcgaggatga agatttgata aagtggaagg cactgtttga 4381 ggaagtccct gagttactca ctgaggcaga gaagaaggaa tgggttgaga aactgactga 4441 agtttctatc agctctgatg ccttcttccc tttccgagat aacgtagaca gagctaaaag 4501 gagtggtgtg gcgtacattg cggctccctc cggttctgct gctgacaaag ttgtgattga 4561 ggcctgcgac gaactgggaa tcatcctcgc tcatacgaac cttcggctct tccaccacgg 4621 atccTACCCA TACGACGTAC CAGATTACGC Ttagtctaga taattctacc gggtagggga 4681 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg 4741 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc 4801 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc 4861 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag 4921 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg 4981 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg 5041 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc 5101 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc 5161 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc 5221 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 5281 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 5341 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5401 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5461 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5521 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5581 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5641 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5701 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5761 tggtgcatga cccgcaagcc cggtgcctga ccgcgtctgg aacaatcaac ctctggatta 5821 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 5881 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 5941 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 6001 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 6061 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 6121 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 6181 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 6241 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 6301 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 6361 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta 6421 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg 6481 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga 6541 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg 6601 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca 6661 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg 6721 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat 6781 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag 6841 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag 6901 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct 6961 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc 7021 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc 7081 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 7141 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 7201 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 7261 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg 7321 ttttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 7381 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 7441 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 7501 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 7561 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 7621 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 7681 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 7741 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 7801 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 7861 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 7921 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 7981 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 8041 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 8101 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 8161 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 8221 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 8281 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 8341 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 8401 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 8461 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 8521 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 8581 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 8641 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 8701 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 8761 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 8821 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 8881 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 8941 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 9001 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 9061 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 9121 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 9181 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 9241 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 9301 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 9361 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 9421 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 9481 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg 9541 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 9601 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 9661 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac 9721 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa 9781 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttttt 9841 aaagaaattg tatttgttaa atatgtacta caaacttagt agt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.