pCMV-Polr3a-3×FLAG-Neo plasmid

pCMV-Polr3a-3×FLAG-Neo plasmid

PVT110625

pCMV-Polr3a-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Polr3a(mouse)-3×FLAG-Neo
Alias: NM_001081247.2;9330175N20Rik; RPC1; RPC155
Gene lenght: 4170
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Polr3a-3×FLAG-Neo plasmid Description

pCMV-Polr3a-3×FLAG-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Polr3a-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccatggt gaaggagcag ttccgcgaga 781 cggatgtggc taagaaaata agccatatct gttttggaat gaagtcccct gaggagatgc 841 gccagcaggc acatatccag gttgtcagta agaacctgta cagccaagac aacaaccacg 901 cccccttgtt gtatggagtg cttgaccata gaatgggtac gagcgaaaaa gatcgtcctt 961 gtgagacctg tgggaagaac ttggcggact gtctcggcca ctatggctac attgatctcg 1021 agctaccgtg ctttcatgtc gggtacttca gggctgtcat tggtatctta cagatgatct 1081 gcaaaacctg ctgccacatt atgctgtccc aggaagagaa gcagcagttt ctagattttc 1141 tgaagaggcc tggcctaacc tacctgcaga agcgaggcct gaagaagaag atctcagata 1201 aatgccggaa gaaaagcact tgtcactact gtggtgcctt taatggtacc gtaaagaaat 1261 gtggtttact gaagataatt catgaaaaat acaagacaaa caaaaaagtc gtggatccca 1321 ttgtatcaaa cttcctgcag tcctttgaga cagccattga gcataacaaa gaggttgaac 1381 ccctgctggg aagggcccag gagaacttga atcctttagt agttctgaac ttgtttaagc 1441 gcatcccagc tgaagatgtc ccgttactcc tgatgaaccc agaatctgga aagccctctg 1501 atttgattct cacccgactt ttggtgcctc ccctgtgtat cagaccgtcc gtcgtgagtg 1561 atctgaagtc tggaaccaat gaagatgacc tgacaatgaa actgacagag atcattttcc 1621 taaatgatgt tattaaaaag caccggatct ccggagccaa gacgcagatg atcatggagg 1681 actgggattt cctgcagctg cagtgtgccc tctacatcaa cagtgagctc tctggcatcc 1741 ctctcaacat ggcacctaag aaatggacgc gaggctttgt gcagcgcctg aaggggaaac 1801 agggtcgatt tagaggtaat ctgtcaggga agagggtgga tttctccggc cgaacagtca 1861 tctcacctga ccccaacctc cggattgatg aagtagccgt gccagttcat gtggccaaaa 1921 ttctaacttt tcctgagaag gtgaacaaag caaacatcaa ctttttgagg aaactggttc 1981 gaaatggccc agatgttcac ccaggagcca attttatcca acaaagacac atgcagatga 2041 agaggttcct gaagtatgga aatcgggaaa agatggctca ggagctcaag ttcggcgaca 2101 ttgtagagag acacctgata gatggagatg tggtcttgtt caaccggcag ccctccctgc 2161 acaaactgag catcatggcc cacctggcca gggttaagcc ccaccggacc ttcagattca 2221 atgaatgtgt ctgcaccccc tacaatgcag actttgatgg ggatgaaatg aaccttcatc 2281 ttccccagac agaggaagcc aaagcagagg cccttgttct catggggacc aaagcgaacc 2341 ttgtaacccc acgaaatggg gagccactca ttgctgccat tcaggacttc ttaacaggtg 2401 cttacctcct caccctcaag gacaccttct ttgaccgagc caaggcttgc cagatcatcg 2461 cttcaatact ggttggcaaa gatgaaaaaa ttaaagtgcg cctcccaccc cctacaatac 2521 taaagcctgt caccctgtgg accggaaagc agatcttcag tgtcatcctc agacctagtg 2581 atgacaatcc agtgcgggcc aacctccgaa ccaagggcaa gcagtactgt ggcaaggggg 2641 aagacctctg cgtcaacgac tcctatgtca ccatccagaa cagtgagttg atgtgcggca 2701 gcatggacaa agggaccctg gggtcggggt ccaagaacaa catcttctac atcttgctac 2761 gagactgggg gcagaatgag gctgccgatg ccatgtctcg gcttgccagg ctggctcctg 2821 tctacctgtc taaccgtggg ttctccatcg ggattggtga tgtcacgcct ggccagggac 2881 tgctgaaagc caagtatgag ctactaaatg ctggctacaa aaaatgtgac gagtacattg 2941 aagccctgaa cacaggcaag ctgcagcagc agccaggctg cacggccgag gagacgcttg 3001 aggctctgat cctgaaggag ctgtctgtga tccgggacca tgctggcagt gcctgcctcc 3061 gcgagctgga caagagcaac agccccctca ccatggccct gtgtggctcc aaaggttcct 3121 tcataaacat atcacagatg attgcctgtg tgggacagca agccatcagt ggctcccggg 3181 ttccagatgg ctttgagaac agatctttgc ctcactttga gaagcattca aagctccctg 3241 ctgccaaagg ctttgtggcc aatagctttt actctggctt aacgcctact gagtttttct 3301 tccacacaat ggccggtcgg gagggtctgg tggacacagc tgtaaagact gctgaaacag 3361 gatacatgca gagaagactg gtcaaatccc tggaagacct ctgctcgcag tacgatctga 3421 cagtgcggag ctccaccggt gacatcatcc agttcatcta tggaggagat ggcttagatc 3481 ctgctgccat ggagggcaaa gacgaaccgc tggagtttaa gcgggttctg gacaacatca 3541 aggcagtgta tccatgtcag actgaacctg ctctcagtaa gaacgacctc accttgaccg 3601 ctgaggccat catgaagaag aatgagttcc tatgctgcca agacagcttc ctgcaggaaa 3661 taaaaacatt cattaaaggg gtgtccgaga agattaagaa aaccagagat aaatatggga 3721 tcaatgataa cggcacaaca gagcctcgtg tgctgtacca gttggaccgt attaccccta 3781 cccaaataga gaagtttctg gagacctgta gggataagta tatgagagca cagatggagc 3841 caggctctgc ggtaggtgca ctctgtgcac agagcattgg tgagcccggc acccagatga 3901 cgctgaagac attccacttt gcaggagtag cctccatgaa catcaccctg ggtgtgcctc 3961 ggatcaaaga gatcatcaac gcttccaagg ccatcagcac tccaattatc acagcacagc 4021 tagacaagga tgacgacgcg gattatgcac gcctggtgaa gggcaggatt gagaaaaccc 4081 tcctggggga gatctcagag tatattgaag aagtgtttct tcctgatgac tgctttattc 4141 ttgtcaagct ctccctggaa cggatccgac ttctgagact ggaggtcaat gctgagacag 4201 tgcggtactc catctgcaca tccaagctcc gagtgaagcc tggtgatgtg gccgttcatg 4261 gggaggctgt ggtgtgtgtc acccccaggg aaaacagcaa gagctccatg tattatgtgc 4321 tgcagtttct caaagaggat ctgcccaagg tggttgtaca aggcatccca gaggtgtcgc 4381 gagctgtcat ccatattgac gagcagagtg ggaaggagaa gttcaagctt ctggtggaag 4441 gtgacaacct gcgggcagtc atggccaccc acggtgtgaa aggcacccgg accacgtcta 4501 ataacaccta tgaggtggaa aaaaccctgg gcattgaggc tgcccggaca actatcatca 4561 acgagatcca gtacacgatg gtcaaccacg gcatgagcat cgacaggagg cacgtgatgc 4621 tgctctctga cctgatgacc tacaagggtg aagtcctggg tataaccagg tttggcctgg 4681 ccaagatgaa ggagagtgtg ttgatgctgg cctcctttga aaagacagct gaccatctct 4741 ttgatgctgc ctactttggg cagaaagact ctgtatgcgg agtgtctgag tgcatcatca 4801 tgggaatccc gatgaacatc ggaactgggc tcttcaagct gttacacaaa gccaacaggg 4861 accccaaacc acccaggaga cccctgatat ttgacacaca tgaattccac atccctcttg 4921 ttacaGGATC CGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA 4981 AGGATGACGA TGACAAGTAG TGAGGGCCCG ATATCTCGAC AATCAACCTC TGGATTACAA 5041 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA 5101 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC 5161 CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG 5221 TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC 5281 CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT 5341 CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT 5401 GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT 5461 TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC 5521 CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG 5581 TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGAAACGGG GGAGGCTAAC TGAAACACGG 5641 AAGGAGACAA TACCGGAAGG AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG 5701 CACGGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT 5761 CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC 5821 CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC 5881 TGCCATAGCA GATCTGCGCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG 5941 CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC 6001 CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC 6061 CCGTCAAGCT CTAAATCGGG GCATCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT 6121 CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC 6181 GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC 6241 TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGGGGAT 6301 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG 6361 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 6421 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 6481 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 6541 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 6601 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG 6661 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 6721 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 6781 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 6841 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 6901 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 6961 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 7021 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 7081 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 7141 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 7201 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 7261 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 7321 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 7381 ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 7441 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 7501 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 7561 GGACTCTGGG GTTCGCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT 7621 CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG 7681 CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT 7741 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC 7801 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT 7861 CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT 7921 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA 7981 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC 8041 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG 8101 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT 8161 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 8221 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 8281 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 8341 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 8401 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 8461 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT 8521 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 8581 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 8641 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 8701 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT 8761 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 8821 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 8881 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 8941 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 9001 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA 9061 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC 9121 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG 9181 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT 9241 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT 9301 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG 9361 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC 9421 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA 9481 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC 9541 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT 9601 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG 9661 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT 9721 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG 9781 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC 9841 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC 9901 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA 9961 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG 10021 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCTCCC 10081 GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT 10141 CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA 10201 ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.