pCMV-L1cam-P2A-EGFP-Neo plasmi

pCMV-L1cam-P2A-EGFP-Neo plasmid

PVT110624

pCMV-L1cam-P2A-EGFP-Neo plasmid Information

Plasmid name: pCMV-L1cam(mouse)-P2A-EGFP-Neo
Alias: NM_008478.3;CD171; L1; N-CAM-L1; NCAM-L1
Gene lenght: 3777
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-L1cam-P2A-EGFP-Neo plasmid Description

pCMV-L1cam-P2A-EGFP-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-L1cam-P2A-EGFP-Neo plasmid Sequence

1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat 61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat 241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 781 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc 841 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag 901 cgtttaaact taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc 961 caccatggtc gtgatgctgc ggtacgtgtg gcctctcctc ctctgcagcc cctgcctgct 1021 catacagatt ccagacgaat ataaaggaca ccatgtgcta gagccacctg tcatcacgga 1081 acagtctcca cggcgcctgg ttgtcttccc aacagatgac ataagcctga aatgtgaagc 1141 cagaggcaga ccccaagtgg agttccgctg gacgaaagat ggcatccact tcaaacccaa 1201 ggaagaattg ggtgtagtgg tgcatgaggc accctattct ggctccttca ccatcgaagg 1261 caacaacagc tttgcccaga ggtttcaggg catctatcgc tgctatgcca gcaataagct 1321 aggaactgcc atgtcgcatg agatccagct cgtggccgag ggtgccccca agtggccgaa 1381 ggagactgta aaacctgtgg aagtggagga aggagaatca gtagttctgc cttgcaaccc 1441 tccacccagt gcagccccac ttaggatcta ctggatgaac agcaagattt tgcacatcaa 1501 acaagatgag cgggtgtcca tgggccagaa tggagaccta tattttgcca atgtgcttac 1561 ctcagacaat cattcagact acatctgcaa tgcccacttc cctggtaccc ggaccatcat 1621 tcaaaaggaa cctattgacc tccgggtcaa gcccaccaac agcatgattg accggaagcc 1681 acgtctgctc tttcccacaa actccagcag ccgcctggta gccttgcagg gccagtcatt 1741 gatcctggag tgcattgctg agggattccc tacacccacc atcaagtggc tgcaccccag 1801 tgacccaatg ccaacagacc gtgttatcta ccaaaaccac aacaagaccc tgcaactact 1861 caatgtgggc gaagaggacg atggcgagta tacctgcctt gctgagaact cgctgggcag 1921 tgcccggcat gcctactatg ttactgtgga agctgcccca tattggctgc agaagcccca 1981 gagccatttg tatggtccag gagagactgc ccgcctagac tgccaagtcc agggcaggcc 2041 ccaaccagag atcacttgga gaatcaacgg aatgtctatg gagacggtga acaaggacca 2101 gaagtaccgg attgagcagg ggtctctgat cttgagtaac gtgcagccaa gtgacacaat 2161 ggtgacccag tgtgaagccc gcaaccagca tgggctcctg ctagccaatg cctacattta 2221 tgttgtccag ctgccagcca ggatcctaac aaaagacaat cagacataca tggcagttga 2281 gggcagtact gcttacttgc tgtgcaaagc ctttggagct cctgttccca gtgtccagtg 2341 gctggatgaa gaaggaacca cagtgcttca ggatgaacga tttttcccct atgccaatgg 2401 aacgctgagc atcagagacc tccaggccaa tgacactgga cgctatttct gccaggctgc 2461 caatgaccag aacaatgtga ccattttggc taacctacag gttaaagaag caacccagat 2521 cacacagggg ccccggagcg caattgagaa gaaaggtgca agggtgacat tcacgtgcca 2581 ggcctccttt gacccctctt tgcaggccag catcacttgg cgtggagatg ggagagacct 2641 acaggaacgt ggggacagtg acaagtattt catagaagat gggaaactag tcatccagag 2701 cctggactac agtgaccagg gcaactacag ttgtgtggcc agcactgaac tggatgaggt 2761 ggagagcagg gcacagctct tagtggtggg gagccctggg ccagtgcctc acctggagct 2821 gtccgaccgc cacctgctga agcagagcca ggtgcacttg tcttggagcc ctgctgaaga 2881 ccacaactct cccattgaga agtatgacat tgaatttgag gacaaggaaa tggctcctga 2941 gaaatggttc agtctgggca aggtgccagg aaatcagacc tctactaccc tcaagctgtc 3001 cccctatgtc cactacacct ttcgggtcac tgccattaac aaatatggtc ctggagaacc 3061 cagccctgtc tctgagactg tggtcacacc tgaggcagcc ccagagaaga accctgtgga 3121 tgtgagaggg gaagggaatg agaccaacaa tatggtcatc acatggaagc cccttcggtg 3181 gatggattgg aatgcccccc agattcagta ccgtgtacag tggcgtccac agggcaagca 3241 ggagacctgg agggaacaga ccgtgagcga ccctttcctg gtggtgtcta acacttccac 3301 atttgtgcct tatgagatca aagtccaggc agtgaacaac cagggcaagg gccctgagcc 3361 ccaggtcacc attggctatt caggggaaga ctacccccag gtgagccctg aacttgaaga 3421 catcacaatc ttcaactcaa gtactgtgct tgtcaggtgg aggcctgtgg acttggccca 3481 ggttaagggc cacctcaagg gatacaatgt aacatactgg tggaagggca gccagagaaa 3541 gcacagcaag aggcatatcc acaaaagcca catagtggta cctgcaaata ccaccagtgc 3601 catcctcagt ggtttgcgcc cttacagctc ttaccatgtg gaggtacagg cctttaatgg 3661 gcggggcttg gggcctgcga gtgaatggac cttcagcacc ccagagggag tgcctggcca 3721 ccctgaggca ttacacctgg agtgtcagtc ggacactagt ctgctactgc actggcagcc 3781 accactcagc cacaatggag tgctcactgg ctacctgctc tcttaccatc ccgtggaagg 3841 ggaaagcaaa gagcagttgt tcttcaacct ttcggaccca gaactccgga ctcataatct 3901 gaccaacctc aaccctgatc tacagtaccg cttccagctt caggccacca cccaacaggg 3961 tcctggtgag gccatcgtgc gtgaaggagg caccatggcc ctgtttggca agccagattt 4021 tggcaacatc tcagccacag caggtgaaaa ctacagcgtg gtctcctggg tccctcggaa 4081 gggccagtgc aatttcaggt tccatatctt gttcaaagcc ttaccagaag ggaaagtgag 4141 ccctgatcac cagcctcagc ctcagtatgt cagctacaat cagagctcct acacacaatg 4201 gaacctacag cctgacacca aatatgagat ccacctgata aaggagaagg tcctcctgca 4261 ccatctggat gtgaagacta atggaactgg ccctgtgcga gtttctacta caggtagctt 4321 tgcctccgag ggctggttca tcgcctttgt cagcgctatc attctcttgc tcctcatcct 4381 gctcatcctc tgcttcatca aacgcagcaa gggtggcaaa tactcagtga aggacaagga 4441 ggacactcag gtagattccg aggcccggcc catgaaagac gagaccttcg gcgagtacag 4501 gtccctggag agtgacaatg aagagaaggc ctttggcagc agccagccat ctctcaacgg 4561 agacatcaaa cccctaggca gtgatgacag cctggctgat tatgggggca gtgtggacgt 4621 ccagttcaat gaggatggct ctttcatcgg ccagtacagt ggcaagaaag agaaggaggc 4681 agcaggaggc aatgacagtt caggggctac ctctcctatc aatcctgcag tagccctaga 4741 aggaagcgga gctactaact tcagcctgct gaagcaggct ggcgacgtgg aggagaaccc 4801 tggacctccg cgggcccggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct 4861 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 4921 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 4981 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg 5041 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 5101 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 5161 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 5221 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 5281 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 5341 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 5401 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 5461 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 5521 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgctcga gtctagaggg 5581 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 5641 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 5701 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 5761 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 5821 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 5881 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 5941 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 6001 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 6061 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 6121 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 6181 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 6241 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 6301 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 6361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 6421 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 6481 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 6541 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 6601 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 6661 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga 6721 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 6781 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 6841 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 6901 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 6961 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 7021 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 7081 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 7141 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 7201 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 7261 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 7321 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 7381 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 7441 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 7501 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 7561 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 7621 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 7681 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 7741 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 7801 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 7861 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 7921 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 7981 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 8041 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 8101 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 8161 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 8221 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 8281 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 8341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 8401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 8461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 8521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 8581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 8641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 8701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 8761 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac 8821 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 8881 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 8941 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 9001 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 9061 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 9121 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 9181 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 9241 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 9301 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 9361 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 9421 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 9481 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 9541 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 9601 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 9661 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 9721 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 9781 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 9841 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 9901 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 9961 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.