pCMV-3×HA-Adrb2-Neo plasmid

pCMV-3×HA-Adrb2-Neo plasmid

PVT110579

pCMV-3×HA-Adrb2-Neo plasmid Information

Plasmid name: pCMV-3×HA-Adrb2(mouse)-Neo
Alias: NM_007420.3;Adrb-2; Badm; Gpcr7;
Gene lenght: 1254
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×HA-Adrb2-Neo plasmid Description

pCMV-3×HA-Adrb2-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-3×HA-Adrb2-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttAAACTT AAGCCACCAT GGCGGCGTAC CCATACGACG TACCTGATTA TGCAACTTAT 961 CCTTATGATG TACCTGATTA TGCTAGCTAT CCTTATGATG TCCCAGACTA CGCATCAGCG 1021 GAAAAGCTTa tggggccaca cgggaacgac agcgacttct tgctggcacc caacggaagc 1081 cgagcgccag accacgacgt cactcaggaa cgggacgaag cgtgggttgt gggcatggcc 1141 atcctcatgt cggttatcgt cctggccatc gtgtttggca acgtgctggt catcacagcc 1201 attgccaagt tcgagcgact acaaaccgtc accaactact tcataatctc cttggcgtgt 1261 gctgatctag tcatgggcct agcggtggtg ccgtttgggg ccagtcacat ccttatgaaa 1321 atgtggaatt ttggcaactt ctggtgcgag ttctggactt ccattgatgt gttgtgcgtc 1381 acagccagca tcgagaccct gtgcgtgatt gcagtggatc gctatgttgc tatcacatcg 1441 cccttcaagt accagagcct gctgaccaag aataaggccc gagtggtcat cctgatggta 1501 tggattgtat ctggccttac ctcctttttg cctatccaga tgcactggta ccgtgccacc 1561 cacaagaaag ctatcgattg ttacaccgag gagacttgct gtgacttctt cacgaaccag 1621 gcctacgcca tcgcgtcctc gattgtgtct ttctacgtgc ccctggtggt gatggtcttt 1681 gtctattccc gggtcttcca ggtggccaaa aggcagctgc agaagataga caaatctgaa 1741 ggaagattcc acgcccaaaa cctcagccag gtggagcagg atgggcggag cggccacgga 1801 ctccgaaggt cctccaagtt ctgcttgaaa gagcacaaag ccctcaagac tttaggcatc 1861 atcatgggca cattcaccct ctgctggctg cccttcttca ttgtcaatat cgtgcacgtt 1921 atcagggaca acctcatccc taaggaagtt tacatcctcc ttaactggtt gggctacgtc 1981 aactctgcct tcaatcctct tatctactgt cggagtccag atttcaggat tgcctttcaa 2041 gagcttctgt gccttcgcag gtcttcttcg aaaacctatg ggaacggcta ctctagcaat 2101 agcaacggca gaacggacta cacaggggag ccaaacactt gtcagctggg gcaggagaga 2161 gaacaggaac tgctgtgtga ggatccccca ggcatggaag gctttgtgaa ctgtcaaggt 2221 actgtgccta gccttagcgt tgactcccaa ggaaggaact gtagtacaaa tgactcgcca 2281 ctgTgaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta 2341 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 2401 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 2461 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 2521 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc 2581 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg 2641 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 2701 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 2761 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 2821 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 2881 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 2941 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 3001 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta 3061 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga 3121 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 3181 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 3241 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 3301 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag 3361 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt 3421 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa 3481 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg 3541 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc 3601 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca 3661 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc 3721 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca 3781 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat 3841 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca 3901 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg 3961 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc 4021 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct 4081 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct 4141 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac 4201 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc 4261 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 4321 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 4381 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 4441 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 4501 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 4561 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 4621 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 4681 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 4741 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 4801 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 4861 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 4921 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 4981 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 5041 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 5101 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 5161 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 5221 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 5281 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 5341 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 5401 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 5461 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 5521 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa 5581 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 5641 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 5701 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 5761 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 5821 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 5881 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 5941 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 6001 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 6061 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 6121 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 6181 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 6241 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 6301 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 6361 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 6421 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 6481 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 6541 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 6601 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 6661 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 6721 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.