pLV3-CMV-SERPINF1-EF1a-CopGFP-

pLV3-CMV-SERPINF1-EF1a-CopGFP-Hyg plasmid

PVT110552

pLV3-CMV-SERPINF1-EF1a-CopGFP-Hyg plasmid Information

Plasmid name: pLV3-CMV-SERPINF1(human)-EF1a-CopGFP-Hyg
Alias: NM_002615.7;EPC-1; OI12; OI6; PEDF; PIG35
Gene lenght: 1257
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SERPINF1-EF1a-CopGFP-Hyg plasmid Description

pLV3-CMV-SERPINF1-EF1a-CopGFP-Hyg It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-SERPINF1-EF1a-CopGFP-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagaGCCACC atgcaggccc tggtgctact cctctgcatt ggagccctcc tcgggcacag 2341 cagctgccag aaccctgcca gccccccgga ggagggctcc ccagaccccg acagcacagg 2401 ggcgctggtg gaggaggagg atcctttctt caaagtcccc gtgaacaagc tggcagcggc 2461 tgtctccaac ttcggctatg acctgtaccg ggtgcgatcc agcacgagcc ccacgaccaa 2521 cgtgctcctg tctcctctca gtgtggccac ggccctctcg gccctctcgc tgggagcgga 2581 gcagcgaaca gaatccatca ttcaccgggc tctctactat gacttgatca gcagcccaga 2641 catccatggt acctataagg agctccttga cacggtcact gccccccaga agaacctcaa 2701 gagtgcctcc cggatcgtct ttgagaagaa gctgcgcata aaatccagct ttgtggcacc 2761 tctggaaaag tcatatggga ccaggcccag agtcctgacg ggcaaccctc gcttggacct 2821 gcaagagatc aacaactggg tgcaggcgca gatgaaaggg aagctcgcca ggtccacaaa 2881 ggaaattccc gatgagatca gcattctcct tctcggtgtg gcgcacttca aggggcagtg 2941 ggtaacaaag tttgactcca gaaagacttc cctcgaggat ttctacttgg atgaagagag 3001 gaccgtgagg gtccccatga tgtcggaccc taaggctgtt ttacgctatg gcttggattc 3061 agatctcagc tgcaagattg cccagctgcc cttgaccgga agcatgagta tcatcttctt 3121 cctgcccctg aaagtgaccc agaatttgac cttgatagag gagagcctca cctccgagtt 3181 cattcatgac atagaccgag aactgaagac cgtgcaggcg gtcctcactg tccccaagct 3241 gaagctgagt tatgaaggcg aagtcaccaa gtccctgcag gagatgaagc tgcaatcctt 3301 gtttgattca ccagacttta gcaagatcac aggcaaaccc atcaagctga ctcaggtgga 3361 acaccgggct ggctttgagt ggaacgagga tggggcggga accaccccca gcccagggct 3421 gcagcctgcc cacctcacct tcccgctgga ctatcacctt aaccagcctt tcatcttcgt 3481 actgagggac acagacacag gggcccttct cttcattggc aagattctgg accccagggg 3541 cccctaagga tccgcggccg cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag 3601 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg 3661 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 3721 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 3781 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac 3841 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc 3901 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga 3961 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc 4021 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt 4081 acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg 4141 gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg 4201 agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga 4261 gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct 4321 tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca 4381 acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga 4441 gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg 4501 gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg 4561 agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc 4621 tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca 4681 tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc 4741 tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg 4801 ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg 4861 gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga 4921 atcccggccc tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg 4981 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt 5041 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt 5101 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag 5161 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg 5221 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg 5281 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg 5341 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc 5401 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg 5461 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg 5521 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact 5581 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc 5641 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg 5701 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga 5761 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg 5821 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct 5881 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc 5941 cgagggcaaa ggaataggtc gacaatcaac ctctggatta caaaatttgt gaaagattga 6001 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 6061 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 6121 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 6181 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 6241 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 6301 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 6361 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 6421 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 6481 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 6541 ccgcctcccc gcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 6601 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct 6661 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 6721 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 6781 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 6841 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 6901 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 6961 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 7021 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta 7081 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 7141 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 7201 ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg 7261 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 7321 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 7381 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 7441 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 7501 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 7561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 7621 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 7681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 7741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 7801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 7861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 7921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 7981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 8041 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 8101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 8161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 8221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 8281 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 8341 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 8401 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 8461 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 8521 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 8581 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 8641 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 8701 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 8761 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 8821 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 8881 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 8941 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 9001 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 9061 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 9121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 9181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 9241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 9301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 9361 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 9421 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat 9481 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 9541 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 9601 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa 9661 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg 9721 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 9781 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 9841 tgtaaaacga cggccagtgc caagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.