pLV3-CMV-Rhoa-EF1a-Hygro plasm

pLV3-CMV-Rhoa-EF1a-Hygro plasmid

PVT110522

pLV3-CMV-Rhoa-EF1a-Hygro plasmid Information

Plasmid name: pLV3-CMV-Rhoa(mouse)-EF1a-Hygro
Alias: NM_016802.5;Arha; Arha1; Arha2
Gene lenght: 582
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Rhoa-EF1a-Hygro plasmid Description

pLV3-CMV-Rhoa-EF1a-Hygro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-Rhoa-EF1a-Hygro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agaGCCACCa tggctgccat caggaagaaa ctggtgattg ttggtgatgg agcttgtggt 2341 aagacatgct tgctcatagt cttcagcaag gaccagttcc cagaggtcta tgtgcccacg 2401 gtgtttgaaa actatgtggc ggatatcgag gtggatggga agcaggtaga gttggcttta 2461 tgggacacag ctggacagga agattatgac cgcctgcggc ctctctctta tccagacacc 2521 gatgttatat tgatgtgttt ttccattgac agccctgata gtttagaaaa catcccagaa 2581 aaatggactc cagaagtcaa gcatttctgt ccaaatgtgc ccatcatcct ggttgggaac 2641 aagaaggacc ttcggaatga cgagcacacg agacgggagt tggccaaaat gaagcaggag 2701 ccggtaaaac ctgaagaagg cagagatatg gcaaacagga ttggcgcttt tgggtacatg 2761 gagtgttcag caaagaccaa agatggagtg agagaggttt ttgagatggc cacgagagct 2821 gctctgcaag ctagacgtgg gaagaaaaag tctgggtgcc tcatcttgtg aggatccgcg 2881 gccgcaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 2941 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 3001 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 3061 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 3121 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 3181 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 3241 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 3301 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 3361 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 3421 ccggcgccta cgtcgacatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc 3481 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc 3541 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg 3601 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc 3661 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg 3721 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg 3781 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc 3841 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg 3901 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg 3961 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg 4021 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca 4081 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct 4141 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg 4201 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct 4261 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg 4321 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg 4381 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca 4441 ctcgtccgag ggcaaaggaa taggtcgaca atcaacctct ggattacaaa atttgtgaaa 4501 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 4561 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 4621 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 4681 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 4741 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 4801 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 4861 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 4921 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 4981 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 5041 tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat 5101 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa 5161 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 5221 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 5281 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 5341 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 5401 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat 5461 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 5521 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc 5581 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 5641 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 5701 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt 5761 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 5821 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 5881 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 5941 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 6001 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 6061 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 6121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 6181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 6241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 6301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 6361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 6421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 6481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 6541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 6601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 6661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 6721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 6781 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6841 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 6901 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 6961 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 7021 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 7081 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 7141 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 7201 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 7261 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 7321 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 7381 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 7441 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 7501 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 7561 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 7621 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 7681 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 7741 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 7801 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 7861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 7921 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 7981 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 8041 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 8101 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 8161 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat 8221 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 8281 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 8341 cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.