pLV3-EF1a-H2B-mCherry-PGK-BSD

pLV3-EF1a-H2B-mCherry-PGK-BSD plasmid

PVT110405

pLV3-EF1a-H2B-mCherry-PGK-BSD plasmid Information

Plasmid name: pLV3-EF1a-H2B-mCherry-PGK-BSD
Alias: NM_021058.4;H2B/r; H2BFR; H2BJ; HIST1H2BJ
Gene lenght: 378
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Red
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF-1α-F1
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-H2B-mCherry-PGK-BSD plasmid Description

pLV3-EF1a-H2B-mCherry-PGK-BSD It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-EF1a-H2B-mCherry-PGK-BSD plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg ccagagccag cgaagtctgc 2521 tcccgccccg aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa 2581 gaagcgcaag cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca 2641 ggtccaccct gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa 2701 cgacattttc gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc 2761 gaccatcacc tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc 2821 caagcacgcc gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaaggatcc 2881 accggtcgcc accatggtga gcaagggcga ggaggataac atggcgatca tcaaggagtt 2941 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 3001 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 3061 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc 3121 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt 3181 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 3241 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc 3301 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta 3361 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg 3421 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg 3481 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt 3541 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 3601 gtaggggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg 3661 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt 3721 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta 3781 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta 3841 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc 3901 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga 3961 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg 4021 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc 4081 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccATGGCC AAGCCTTTGT 4141 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT 4201 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG 4261 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG 4321 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA 4381 TCTTGAGCCC CTGCGGACGG TGCCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG 4441 CCATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT 4501 CTGGTTATGT GTGGGAGGGC TAAgtcgaca atcaacctct ggattacaaa atttgtgaaa 4561 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 4621 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 4681 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 4741 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 4801 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 4861 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 4921 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 4981 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 5041 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 5101 tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat 5161 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa 5221 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 5281 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 5341 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 5401 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 5461 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat 5521 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 5581 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc 5641 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 5701 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 5761 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt 5821 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 5881 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 5941 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 6001 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 6061 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 6121 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 6181 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 6241 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 6301 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 6361 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 6421 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 6481 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 6541 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 6601 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 6661 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 6721 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 6781 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 6841 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6901 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 6961 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 7021 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 7081 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 7141 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 7201 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 7261 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 7321 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 7381 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 7441 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 7501 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 7561 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 7621 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 7681 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 7741 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 7801 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 7861 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 7921 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 7981 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 8041 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 8101 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 8161 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 8221 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat 8281 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 8341 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 8401 cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.