pLV3-CMV-RBP1-3×FLAG-CopGFP-Pu

pLV3-CMV-RBP1-3×FLAG-CopGFP-Puro plasmid

PVT110398

pLV3-CMV-RBP1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-RBP1(human)-3×FLAG-CopGFP-Puro
Alias: NM_002899.5;CRABP-I; CRBP; CRBP1; CRBPI; hCRBP1; RBPC
Gene lenght: 591
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-RBP1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-RBP1-3×FLAG-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-RBP1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg atcctcccgc aggctttgtg 2341 cgcgctggga atccagctgt cgccgccccg cagagccccc tgtccccgga gggcgctcat 2401 ttccgggccg cccaccaccc gcgtagcacc ggcagccgct gtcccggcag tctccagccg 2461 tcccgcccgc ttgtggccaa ctggctccag tcactccccg aaatgccagt cgacttcact 2521 gggtactgga agatgttggt caacgagaat ttcgaggagt acctgcgcgc cctcgacgtc 2581 aatgtggcct tgcgcaaaat cgccaacttg ctgaagccag acaaagagat cgtgcaggac 2641 ggtgaccata tgatcatccg cacgctgagc acttttagga actacatcat ggacttccag 2701 gttgggaagg agtttgagga ggatctgaca ggcatagatg accgcaagtg catgacaaca 2761 gtgagctggg acggagacaa gctccagtgt gtgcagaagg gtgagaagga ggggcgtggc 2821 tggacccagt ggatcgaggg tgatgagctg cacctggaga tgagagtgga aggtgtggtc 2881 tgcaagcaag tattcaagaa ggtgcaggga tccGACTACA AAGACCATGA CGGTGATTAT 2941 AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGt agcggccgcG aaggatctgc 3001 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 3061 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa 3121 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 3181 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag 3241 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca 3301 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc 3361 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc 3421 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc 3481 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta 3541 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc 3601 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag 3661 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac 3721 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac 3781 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag 3841 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg 3901 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac 3961 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg 4021 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg 4081 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc 4141 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag 4201 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat 4261 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt 4321 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg 4381 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 4441 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 4501 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 4561 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 4621 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 4681 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 4741 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 4801 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 4861 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 4921 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa 4981 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 5041 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 5101 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 5161 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 5221 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 5281 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 5341 gtgttgtcgg ggaaATCATC GTCCTTTCCT tggctgctcg cctgtgttgc cacctggatt 5401 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 5461 cgcggcctgc tgccggctct gcggcctctt ccgcgtctTc gccttcgccc tcagacgagt 5521 cggatctccc tttggGccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc 5581 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 5641 ccaacgaaGa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 5701 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 5761 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 5821 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 5881 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 5941 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 6001 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct 6061 ctagctaTCC CGCCCCTAAC TCCGCCCAtc ccgcccctaa ctccgcccag ttccgcccat 6121 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 6181 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac 6241 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 6301 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 6361 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 6421 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 6481 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 6541 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 6601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 6661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 6721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 6781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 6841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 6901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 6961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 7021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 7081 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 7141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 7201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 7261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 7321 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 7381 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 7441 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 7501 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 7561 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 7621 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 7681 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 7741 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 7801 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 7861 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 7921 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 7981 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 8041 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 8101 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 8161 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 8221 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 8281 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 8341 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8401 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 8461 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 8521 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 8581 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 8641 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 8701 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 8761 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 8821 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 8881 g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.