pCMV-PCYT2-HA-Neo plasmid

pCMV-PCYT2-HA-Neo plasmid

PVT110386

pCMV-PCYT2-HA-Neo plasmid Information

Plasmid name: pCMV-PCYT2(human)-HA-Neo
Alias: NM_001184917.3;ET; SPG82
Gene lenght: 1221
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-PCYT2-HA-Neo plasmid Description

pCMV-PCYT2-HA-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-PCYT2-HA-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgatccggaa 961 cgggcgcggg gctgcaggcg gcgcagagca gccgggcccg gggggcaggc gcgccgtgag 1021 ggtgtggtgc gatggctgct atgacatggt gcattacggc cactccaacc agctgcgcca 1081 ggcacgggcc atgggtgact acctcatcgt aggcgtgcac accgatgagg agatcgccaa 1141 gcacaagggg cccccggtgt tcactcagga ggagagatac aagatggtgc aggccatcaa 1201 atgggtggac gaggtggtgc cagcggctcc ctacgtcact acactagaga ccctggacaa 1261 atacaactgt gacttctgtg ttcacggcaa tgacatcacc ctgactgtag atggccggga 1321 cacctatgag gaagtaaagc aggctgggag gtacagagaa tgcaagcgca cgcaaggggt 1381 gtccaccaca gacctcgtgg gccgcatgct gctggtaacc aaagcccatc acagcagcca 1441 ggagatgtcc tctgagtacc gggagtatgc agacagtttt ggcaagcccc ctcacccgat 1501 acccgccggg gacatacttt cctcagaagg ctgctcccag tgccctggtg ggcggaaccc 1561 ctggaccggg gtatcccagt tcctgcagac atctcagaag atcatccagt ttgcttctgg 1621 gaaggagccc cagccagggg agacagtcat ctatgtggct ggtgccttcg acctgttcca 1681 catcgggcat gtggacttcc tggagaaggt gcacaggctg gcagagaggc cctacatcat 1741 cgcgggctta cactttgacc aggaggtcaa tcactacaag gggaagaact accccatcat 1801 gaatctgcat gaacggactc tgagcgtgct ggcctgccgg tacgtgtcag aagtggtgat 1861 tggagccccg tacgcggtca cagcagagct cctaagtcac ttcaaggtgg acctggtgtg 1921 tcacggcaag acagaaatta tccctgacag ggatggctcc gacccatacc aggagcccaa 1981 gagaaggggc atcttccgtc agattgacag tggcagcaac ctcaccacag acctcatcgt 2041 ccagcggatc atcaccaaca ggttggagta tgaggcgcga aaccagaaga aggaagccaa 2101 ggagctggcc ttcctggagg ctgccaggca gcaggcggca cagcccctgg gggagcgcga 2161 tggtgacttc ctcgagTACC CATACGACGT ACCAGATTAC GCTtagtcta gagggcccta 2221 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt 2281 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 2341 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 2401 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 2461 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct 2521 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 2581 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 2641 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt 2701 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 2761 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 2821 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 2881 attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga 2941 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa 3001 gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 3061 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 3121 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 3181 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 3241 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 3301 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat 3361 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 3421 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 3481 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 3541 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 3601 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 3661 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 3721 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 3781 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 3841 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 3901 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 3961 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 4021 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 4081 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 4141 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 4201 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 4261 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 4321 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 4381 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 4441 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 4501 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 4561 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 4621 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 4681 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 4741 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 4801 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 4861 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 4921 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 4981 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 5041 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 5101 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 5161 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 5221 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 5281 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 5341 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 5401 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 5461 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 5521 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 5581 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 5641 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 5701 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 5761 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 5821 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 5881 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 5941 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 6001 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 6061 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 6121 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 6181 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 6241 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 6301 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 6361 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 6421 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 6481 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 6541 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 6601 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 6661 acctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.