pLV3-CMV-Phb1-3×FLAG-CopGFP-Pu

pLV3-CMV-Phb1-3×FLAG-CopGFP-Puro plasmid

PVT110315

pLV3-CMV-Phb1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Phb1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_008831.5;Bap32; Phb
Gene lenght: 816
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Phb1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Phb1-3×FLAG-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-Phb1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ctgccaaagt gtttgagtcc 2341 attggaaagt tcggcctggc gttggcagtt gcaggaggcg tggtgaactc tgctttgtat 2401 aatgtggatg ctggacacag agctgtcatc tttgaccgat tccgtggcgt acaggacatt 2461 gtggtcgggg aagggactca tttcctcatc ccttgggtac agaaaccaat tatctttgac 2521 tgccgctctc gaccacggaa cgtaccagtc atcactggta gcaaagactt gcagaacgtc 2581 aatatcacac tgcgaatcct cttccggccg gtggccagcc agcttcctcg tatctacacc 2641 agcattggcg aggactatga tgagcgggtc ctgccttcta tcaccacaga gatcctcaag 2701 tcggtggtgg ctcgattcga tgctggagaa ttgattaccc agcgagagct ggtctccagg 2761 caggtgagcg atgacctcac agagcgagcg gcaacatttg ggcttatcct ggatgacgtg 2821 tccctgacac atctgacctt cgggaaggag ttcacagagg cagtagaagc caaacaggtg 2881 gctcagcagg aagcggagag agccagattt gtggtggaaa aggctgagca gcagaagaag 2941 gcagccatca tctctgctga gggtgactcc aaggccgctg agctgatcgc caactcgctg 3001 gccacggcag gcgacggcct gatcgagctg cggaagctgg aggccgccga ggacattgcg 3061 taccagctct cccgctctcg gaacatcacc tacctgccag cgggccagtc cgtgcttctc 3121 cagcttcccc agggatccGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC 3181 GACTACAAGG ATGACGATGA CAAGtagcgg ccgcGaagga tctgcgatcg ctccggtgcc 3241 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 3301 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 3361 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 3421 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 3481 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 3541 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 3601 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 3661 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 3721 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag 3781 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac 3841 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc 3901 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg 3961 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg 4021 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc 4081 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag 4141 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc 4201 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc 4261 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac 4321 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc 4381 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc 4441 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc 4501 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt 4561 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc 4621 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 4681 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 4741 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 4801 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 4861 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 4921 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 4981 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 5041 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 5101 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 5161 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg 5221 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 5281 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 5341 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 5401 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 5461 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 5521 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaA 5581 TCATCGTCCT TTCCTtggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 5641 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 5701 gctctgcggc ctcttccgcg tctTcgcctt cgccctcaga cgagtcggat ctccctttgg 5761 Gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 5821 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaGataaga 5881 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 5941 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 6001 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 6061 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 6121 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 6181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 6241 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc taTCCCGCCC 6301 CTAACTCCGC CCAtcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 6361 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 6421 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat 6481 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 6541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 6601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 6661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 6721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 6781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 6841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 6901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 6961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 7021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 7081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 7141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 7201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 7261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 7321 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 7381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 7441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 7501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 7561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 7621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 7681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 7741 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 7801 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 7861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 7921 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 7981 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 8041 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 8101 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 8161 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 8221 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 8281 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 8341 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 8401 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 8461 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 8521 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 8581 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 8641 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 8701 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 8761 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 8821 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 8881 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 8941 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 9001 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 9061 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.