pDC316-MBH-mNeonGreen-CMV-DDB1

pDC316-MBH-mNeonGreen-CMV-DDB1-FLAG plasmid

PVT110240

pDC316-MBH-mNeonGreen-CMV-DDB1-FLAG plasmid Information

Plasmid name: pDC316-MBH-mNeonGreen-CMV-DDB1(human)-FLAG
Alias: NM_001923.5;DDBA; UV-DDB1; WHIKERS; XAP1; XPCE; XPE;XPE-BF
Gene lenght: 3420
Host: Mammalian cells,Adenovirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pDC316-MBH-mNeonGreen-CMV-DDB1-FLAG plasmid Description

pDC316-MBH-mNeonGreen-CMV-DDB1-FLAG Plasmid suitable for gene expression in mammalian cells, also suitable for adenovirus packaging.

pDC316-MBH-mNeonGreen-CMV-DDB1-FLAG plasmid Sequence

1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 421 gggtcaaagt tggcgtttta ttattatagt cagtctagag atatactgag tcattaggga 481 ctttccaatg ggttttgccc agtacataag gtcaataggg gtgaatcaac aggaaagtcc 541 cattggagcc aagtacactg agtcaatagg gactttccat tgggttttgc ccagtacaaa 601 aggtcaatag ggggtgagtc aatgggtttt tcccattatt ggcacgtaca taaggtcaat 661 aggggtgagt cattgggttt ttccagccaa tttaattaaa acgccatgta ctttcccacc 721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat 781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg 841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cacgcattct 901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca 961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcAT GGTGAGCAAG GGCGAGGAGG 1021 ATAACATGGC cTCTcTCCCA GCGACACATG AGTTACACAT CTTTGGCTCC ATCAACGGTG 1081 TGGACTTTGA CATGGTGGGT CAGGGCACCG GCAATCCAAA TGATGGTTAT GAGGAGTTAA 1141 ACCTGAAGTC CACCAAGGGT GACCTCCAGT TCTCCCCCTG GATTCTGGTC CCTCATATCG 1201 GGTATGGCTT CCATCAGTAC CTGCCCTACC CTGACGGGAT GTCGCCTTTC CAGGCCGCCA 1261 TGGTAGATGG CTCCGGATAC CAAGTCCATC GCACAATGCA GTTTGAAGAT GGTGCCTCCC 1321 TTACTGTTAA CTACCGCTAC ACCTACGAGG GAAGCCACAT CAAAGGAGAG GCCCAGGTGA 1381 AGGGGACTGG TTTCCCTGCT GACGGTCCTG TGATGACCAA CTCGCTGACC GCTGCGGACT 1441 GGTGCAGGTC GAAGAAGACT TACCCCAACG ACAAAACCAT CATCAGTACC TTTAAGTGGA 1501 GTTACACCAC TGGAAATGGC AAGCGCTACC GGAGCACTGC GCGGACCACC TACACCTTTG 1561 CCAAGCCAAT GGCGGCTAAC TATCTGAAGA ACCAGCCGAT GTACGTGTTC CGTAAGACGG 1621 AGCTCAAGCA CTCCAAGACC GAGCTCAACT TCAAGGAGTG GCAAAAGGCC TTTACCGATG 1681 TGATGGGCAT GGACGAGCTG TACAAGtgat cataatcagc cataccacat ttgtagaggt 1741 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 1801 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 1861 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 1921 catcaatgta tcttaacgcg ttgacattga ttattgacta gttattaata gtaatcaatt 1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 2521 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact 2581 cactataggg agacccaagc tggctagcGC CACCATGTCG TACAACTACG TGGTAACGGC 2641 CCAGAAGCCC ACCGCCGTGA ACGGCTGCGT GACCGGACAC TTTACTTCGG CCGAAGACTT 2701 AAACCTGTTG ATTGCCAAAA ACACGAGATT AGAGATCTAT GTGGTCACCG CCGAGGGGCT 2761 TCGGCCCGTC AAAGAGGTGG GCATGTATGG GAAGATTGCG GTCATGGAGC TTTTCAGGCC 2821 CAAGGGGGAG AGCAAGGACC TGCTGTTTAT CTTGACAGCG AAGTACAATG CCTGCATCCT 2881 GGAGTATAAA CAGAGTGGCG AGAGCATTGA CATCATTACG CGAGCCCATG GCAATGTCCA 2941 GGACCGCATT GGCCGCCCCT CAGAGACCGG CATTATTGGC ATCATTGACC CTGAGTGCCG 3001 GATGATTGGC CTGCGTCTCT ATGATGGCCT TTTCAAGGTT ATTCCACTAG ATCGCGATAA 3061 TAAAGAACTC AAGGCCTTCA ACATCCGCCT GGAGGAGCTG CATGTCATTG ATGTCAAGTT 3121 CCTATATGGT TGCCAAGCAC CTACTATTTG CTTTGTCTAC CAGGACCCTC AGGGGCGGCA 3181 CGTAAAAACC TATGAGGTGT CTCTCCGAGA AAAGGAATTC AATAAGGGCC CTTGGAAACA 3241 GGAAAATGTC GAAGCTGAAG CTTCCATGGT GATCGCAGTC CCAGAGCCCT TTGGGGGGGC 3301 CATCATCATT GGACAGGAGT CAATCACCTA TCACAATGGT GACAAATACC TGGCTATTGC 3361 CCCTCCTATC ATCAAGCAAA GCACGATTGT GTGCCACAAT CGAGTGGACC CTAATGGCTC 3421 AAGATACCTG CTGGGAGACA TGGAAGGCCG GCTCTTCATG CTGCTTTTGG AGAAGGAGGA 3481 ACAGATGGAT GGCACCGTCA CTCTCAAGGA TCTCCGTGTA GAACTCCTTG GAGAGACCTC 3541 TATTGCTGAG TGCTTGACAT ACCTTGATAA TGGTGTTGTG TTTGTCGGGT CTCGCCTGGG 3601 TGACTCCCAG CTTGTGAAGC TCAACGTTGA CAGTAATGAA CAAGGCTCCT ATGTAGTGGC 3661 CATGGAAACC TTTACCAACT TAGGACCCAT TGTCGATATG TGCGTGGTGG ACCTGGAGAG 3721 GCAGGGGCAG GGGCAGCTGG TCACTTGCTC TGGGGCTTTC AAGGAAGGTT CTTTGCGGAT 3781 CATCCGGAAT GGAATTGGAA TCCACGAGCA TGCCAGCATT GACTTACCAG GCATCAAAGG 3841 ATTATGGCCA CTGCGGTCTG ACCCTAATCG TGAGACTGAT GACACTTTGG TGCTCTCTTT 3901 TGTGGGCCAG ACAAGAGTTC TCATGTTAAA TGGAGAGGAG GTAGAAGAAA CCGAACTGAT 3961 GGGTTTCGTG GATGATCAGC AGACTTTCTT CTGTGGCAAC GTGGCTCATC AGCAGCTTAT 4021 CCAGATCACT TCAGCATCGG TGAGGTTGGT CTCTCAAGAA CCCAAAGCTC TGGTCAGTGA 4081 ATGGAAGGAG CCTCAGGCCA AGAACATCAG TGTGGCCTCC TGCAATAGCA GCCAGGTGGT 4141 GGTGGCTGTA GGCAGGGCCC TCTACTATCT GCAGATCCAT CCTCAGGAGC TCCGGCAGAT 4201 CAGCCACACA GAGATGGAAC ATGAAGTGGC TTGCTTGGAC ATCACCCCAT TAGGAGACAG 4261 CAATGGACTG TCCCCTCTTT GTGCCATTGG CCTCTGGACG GACATCTCGG CTCGTATCTT 4321 GAAGTTGCCC TCTTTTGAAC TACTGCACAA GGAGATGCTG GGTGGAGAGA TCATTCCTCG 4381 CTCCATCCTG ATGACCACCT TTGAGAGTAG CCATTACCTC CTTTGTGCCT TGGGAGATGG 4441 AGCGCTTTTC TACTTTGGGC TCAACATTGA GACAGGTCTG TTGAGCGACC GTAAGAAGGT 4501 GACTTTGGGC ACCCAGCCCA CCGTATTGAG GACTTTTCGT TCTCTTTCTA CCACCAACGT 4561 CTTTGCTTGT TCTGACCGCC CCACTGTCAT CTATAGCAGC AACCACAAAT TGGTCTTCTC 4621 AAATGTCAAC CTCAAGGAAG TGAACTACAT GTGTCCCCTC AATTCAGATG GCTATCCTGA 4681 CAGCCTGGCG CTGGCCAACA ATAGCACCCT CACCATTGGC ACCATCGATG AGATCCAGAA 4741 GCTGCACATT CGCACAGTTC CCCTCTATGA GTCTCCAAGG AAGATCTGCT ACCAGGAAGT 4801 GTCCCAGTGT TTCGGGGTCC TCTCCAGCCG CATTGAAGTC CAAGACACGA GTGGGGGCAC 4861 GACAGCCTTG AGGCCCAGCG CTAGCACCCA GGCTCTGTCC AGCAGTGTAA GCTCCAGCAA 4921 GCTGTTCTCC AGCAGCACTG CTCCTCATGA GACCTCCTTT GGAGAAGAGG TGGAGGTGCA 4981 CAACCTACTT ATCATTGACC AACACACCTT TGAAGTGCTT CATGCCCACC AGTTTCTGCA 5041 GAATGAATAT GCCCTCAGTC TGGTTTCCTG CAAGCTGGGC AAAGACCCCA ACACTTACTT 5101 CATTGTGGGC ACAGCAATGG TGTATCCTGA AGAGGCAGAG CCCAAGCAGG GTCGCATTGT 5161 GGTCTTTCAG TATTCGGATG GAAAACTACA GACTGTGGCT GAAAAGGAAG TGAAAGGGGC 5221 CGTGTACTCT ATGGTGGAAT TTAACGGGAA GCTGTTAGCC AGCATCAATA GCACGGTGCG 5281 GCTCTATGAG TGGACAACAG AGAAGGAGCT GCGCACTGAG TGCAACCACT ACAACAACAT 5341 CATGGCCCTC TACCTGAAGA CCAAGGGCGA CTTCATCCTG GTGGGCGACC TTATGCGCTC 5401 AGTGCTGCTG CTTGCCTACA AGCCCATGGA AGGAAACTTT GAAGAGATTG CTCGAGACTT 5461 TAATCCCAAC TGGATGAGTG CTGTGGAAAT CTTGGATGAT GACAATTTTC TGGGGGCTGA 5521 AAATGCCTTT AACTTGTTTG TGTGTCAAAA GGATAGCGCT GCCACCACTG ACGAGGAGCG 5581 GCAGCACCTC CAGGAGGTTG GTCTTTTCCA CCTGGGCGAG TTTGTCAATG TCTTTTGCCA 5641 CGGCTCTCTG GTAATGCAGA ATCTGGGTGA GACTTCCACC CCCACACAAG GCTCGGTGCT 5701 CTTCGGCACG GTCAACGGCA TGATAGGGCT GGTGACCTCA CTGTCAGAGA GCTGGTACAA 5761 CCTCCTGCTG GACATGCAGA ATCGACTCAA TAAAGTCATC AAAAGTGTGG GGAAGATCGA 5821 GCACTCCTTC TGGAGATCCT TTCACACCGA GCGGAAGACA GAACCAGCCA CAGGTTTCAT 5881 CGACGGTGAC TTGATTGAGA GTTTCCTGGA TATTAGCCGC CCCAAGATGC AGGAGGTGGT 5941 GGCAAACCTA CAGTATGACG ATGGCAGCGG TATGAAGCGA GAGGCCACTG CAGACGACCT 6001 CATCAAGGTT GTGGAGGAGC TAACTCGGAT CCATGATTAC AAGGATGACG ACGATAAGTA 6061 Ggggccctct agactcgagc ggccgccact gtgctggata tctgcagaat tgatccgagc 6121 tcggtaccaa gcttaagttt aaaccgctga tcagcctcga ctgtgccttc tagttgccag 6181 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 6241 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 6301 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 6361 gctggggatg cggtgggctc tatgggtcga cttcgagcaa cttgtttatt gcagcttata 6421 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 6481 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcgtctagc 6541 atcgaagatc caataacttc gtatagcata cattatacga agttataagt agcttggcgt 6601 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 6661 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 6721 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 6781 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 6841 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 6901 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 6961 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 7021 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 7081 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 7141 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 7201 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 7261 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 7321 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 7381 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 7441 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 7501 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 7561 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 7621 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 7681 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 7741 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 7801 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 7861 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 7921 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 7981 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 8041 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 8101 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 8161 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 8221 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 8281 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 8341 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 8401 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 8461 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 8521 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 8581 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 8641 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 8701 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 8761 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcactctag 8821 gcaaaatagc accctcccgc tccagaacaa catacagcgc ttcacagcgg cagcctaaca 8881 gtcagcctta ccagtaaaaa agaaaaccta ttaaaaaaac accactcgac acggcaccag 8941 ctcaatcagt cacagtgtaa aaaagggcca agtgcagagc gagtatatat aggactaaaa 9001 aatgacgtaa cggttaaagt ccacaaaaaa cacccagaaa accgcacgcg aacctacgcc 9061 cagaaacgaa agccaaaaaa cccacaactt cctcaaatcg tcacttccgt tttcccacgt 9121 tacgtaactt cccattttaa gaaaactaca attcccaaca catacaagtt actccgccct 9181 aaaacctacg tcacccgccc cgttcccacg ccccgcgcca cgtcacaaac tccaccccct 9241 cattatcata ttggcttcaa tccaaaataa ggtata

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.