pDC316-CMV-Flag-PH-NLS plasmid

pDC316-CMV-Flag-PH-NLS plasmid

PVT110036

pDC316-CMV-Flag-PH-NLS plasmid Information

Plasmid name: pDC316-CMV-Flag-PH-NLS
Alias:
Gene lenght:
Host: Mammalian cells,Adenovirus
Use(s): Gene expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pDC316-CMV-Flag-PH-NLS plasmid Description

pDC316-CMV-Flag-PH-NLS Plasmid suitable for gene expression in mammalian cells, also suitable for adenovirus packaging.

pDC316-CMV-Flag-PH-NLS plasmid Sequence

1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 421 gggtcaaagt tggcgtttta ttattatagt cagtctagag atatactgag tcattaggga 481 ctttccaatg ggttttgccc agtacataag gtcaataggg gtgaatcaac aggaaagtcc 541 cattggagcc aagtacactg agtcaatagg gactttccat tgggttttgc ccagtacaaa 601 aggtcaatag ggggtgagtc aatgggtttt tcccattatt ggcacgtaca taaggtcaat 661 aggggtgagt cattgggttt ttccagccaa tttaattaaa acgccatgta ctttcccacc 721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat 781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg 841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cacgcattct 901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca 961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattctc ataatcagcc ataccacatt 1021 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa 1081 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag 1141 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 1201 gtccaaactc atcaatgtat cttaacgcgt tgacattgat tattgactag ttattaatag 1261 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt 1321 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg 1381 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat 1441 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct 1501 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg 1561 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg 1621 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc 1681 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa 1741 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc 1801 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt 1861 aatacgactc actataggga gacccaagct ggctagcgtt taaacGCCAC CATGGATTAC 1921 AAGGATGACG ACGATAAGTC TGGTGGCGGA GGCTCGGGCG GAGGTGGGTC GGGTGGCGGC 1981 GGATCAatgc tgggcctgaa gacaagcatc atcggccggc gggtcatcta cttccaggag 2041 atcaccagca ccaacgagtt cgccaagacc agctacctgg aggagggaac cgtgatcgtg 2101 gccgataagc agaccatggg ccacggaggc ctgaacagaa agtgggagtc cccagaagga 2161 ggcctgtggc tgtctatcgt gctcagccct aaagtgcccc agaaggacct gcctaagatc 2221 gtgttcctgg gagcagtggg cgtggtggaa accctgaagg agttcagcat cgacggcaga 2281 atcaagtggc ccaacgacgt gctcgtgaac tacaagaaga tcgccggcgt gctggtcgag 2341 ggcaaaggcg acaagatcgt gctgggcatc ggcctgaacg tgaacaacaa ggtgcccaac 2401 ggcgccacaa gcatgaaact ggagctggga tcagaagtgc ctctgctgag cgtgttcagg 2461 agcctgatca ccaacctgga ccggctgtac ctgaacttcc tgaagaaccc catggacatc 2521 ctgaacctcg tgcgggacaa catgatcctg ggcgtgagag tgaagatcct gggcgacggc 2581 agcttcgagg gcatcgccga ggatatcgac gacttcgggc ggctgatcat caggctggac 2641 agcggcgagg tcaagaaggt catctacggc gacgtgtccc tgagattcct gctcgagggt 2701 ggtggtggtt caagccccaa gaagaagaga aaggtgTAAg ggccctctag actcgagcgg 2761 ccgccactgt gctggatatc tgcagaattg atccgagctc ggtaccaagc ttaagtttaa 2821 accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 2881 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 2941 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 3001 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 3061 tgggtcgact tcgagcaact tgtttattgc agcttataat ggttacaaat aaagcaatag 3121 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 3181 actcatcaat gtatcttatc atgtctggat cgtctagcat cgaagatcca ataacttcgt 3241 atagcataca ttatacgaag ttataagtag cttggcgtaa tcatggtcat agctgtttcc 3301 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 3361 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 3421 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 3481 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 3541 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 3601 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 3661 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 3721 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 3781 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 3841 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 3901 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 3961 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 4021 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 4081 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 4141 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 4201 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 4261 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 4321 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 4381 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 4441 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 4501 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 4561 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 4621 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 4681 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 4741 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 4801 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 4861 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 4921 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 4981 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 5041 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 5101 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 5161 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 5221 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 5281 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 5341 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 5401 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 5461 catgacatta acctataaaa ataggcgtat cactctaggc aaaatagcac cctcccgctc 5521 cagaacaaca tacagcgctt cacagcggca gcctaacagt cagccttacc agtaaaaaag 5581 aaaacctatt aaaaaaacac cactcgacac ggcaccagct caatcagtca cagtgtaaaa 5641 aagggccaag tgcagagcga gtatatatag gactaaaaaa tgacgtaacg gttaaagtcc 5701 acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca gaaacgaaag ccaaaaaacc 5761 cacaacttcc tcaaatcgtc acttccgttt tcccacgtta cgtaacttcc cattttaaga 5821 aaactacaat tcccaacaca tacaagttac tccgccctaa aacctacgtc acccgccccg 5881 ttcccacgcc ccgcgccacg tcacaaactc caccccctca ttatcatatt ggcttcaatc 5941 caaaataagg tata

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.