pCDNA5-CMV-ACVRL1-Hibit-Hyg pl

pCDNA5-CMV-ACVRL1-Hibit-Hyg plasmid

PVT109935

pCDNA5-CMV-ACVRL1-Hibit-Hyg plasmid Information

Plasmid name: pCDNA5-CMV-ACVRL1(human)-Hibit-Hyg
Alias: NM_000020.3;ACVRLK1; ALK-1; ALK1; HHT; HHT2; ORW2;SKR3; TSR-I
Gene lenght: 1509
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCDNA5-CMV-ACVRL1-Hibit-Hyg plasmid Description

pCDNA5-CMV-ACVRL1-Hibit-Hyg It is a plasmid suitable for gene expression in mammalian cells.

pCDNA5-CMV-ACVRL1-Hibit-Hyg plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CCCTATCAGT GATAGAGATC 841 TCCCTATCAG TGATAGAGAT CGTCGACGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA 901 GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA 961 CTCTAGCGTT TAAACTTAAG CTTgccacca tgaccttggg ctcccccagg aaaggccttc 1021 tgatgctgct gatggccttg gtgacccagg gagaccctgt gaagccgtct cggggcccgc 1081 tggtgacctg cacgtgtgag agcccacatt gcaaggggcc tacctgccgg ggggcctggt 1141 gcacagtagt gctggtgcgg gaggagggga ggcaccccca ggaacatcgg ggctgcggga 1201 acttgcacag ggagctctgc agggggcgcc ccaccgagtt cgtcaaccac tactgctgcg 1261 acagccacct ctgcaaccac aacgtgtccc tggtgctgga ggccacccaa cctccttcgg 1321 agcagccggg aacagatggc cagctggccc tgatcctggg ccccgtgctg gccttgctgg 1381 ccctggtggc cctgggtgtc ctgggcctgt ggcatgtccg acggaggcag gagaagcagc 1441 gtggcctgca cagcgagctg ggagagtcca gtctcatcct gaaagcatct gagcagggcg 1501 acagcatgtt gggggacctc ctggacagtg actgcaccac agggagtggc tcagggctcc 1561 ccttcctggt gcagaggaca gtggcacggc aggttgcctt ggtggagtgt gtgggaaaag 1621 gccgctatgg cgaagtgtgg cggggcttgt ggcacggtga gagtgtggcc gtcaagatct 1681 tctcctcgag ggatgaacag tcctggttcc gggagactga gatctataac acagtgttgc 1741 tcagacacga caacatccta ggcttcatcg cctcagacat gacctcccgc aactcgagca 1801 cgcagctgtg gctcatcacg cactaccacg agcacggctc cctctacgac tttctgcaga 1861 gacagacgct ggagccccat ctggctctga ggctagctgt gtccgcggca tgcggcctgg 1921 cgcacctgca cgtggagatc ttcggtacac agggcaaacc agccattgcc caccgcgact 1981 tcaagagccg caatgtgctg gtcaagagca acctgcagtg ttgcatcgcc gacctgggcc 2041 tggctgtgat gcactcacag ggcagcgatt acctggacat cggcaacaac ccgagagtgg 2101 gcaccaagcg gtacatggca cccgaggtgc tggacgagca gatccgcacg gactgctttg 2161 agtcctacaa gtggactgac atctgggcct ttggcctggt gctgtgggag attgcccgcc 2221 ggaccatcgt gaatggcatc gtggaggact atagaccacc cttctatgat gtggtgccca 2281 atgaccccag ctttgaggac atgaagaagg tggtgtgtgt ggatcagcag acccccacca 2341 tccctaaccg gctggctgca gacccggtcc tctcaggcct agctcagatg atgcgggagt 2401 gctggtaccc aaacccctct gcccgactca ccgcgctgcg gatcaagaag acactacaaa 2461 aaattagcaa cagtccagag aagcctaaag tgattcaaGT GAGCGGCTGG CGGCTGTTCA 2521 AGAAGATTAG CTGAGGATCC ACTAGTCCAG TGTGGTGGAA TTCTGCAGAT ATCCAGCACA 2581 GTGGCGGCCG CTCGAGTCTA GAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC 2641 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG 2701 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG 2761 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA 2821 CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG 2881 CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 2941 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 3001 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 3061 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 3121 GGGTGATGGT TCACGTACCT AGAAGTTCCT ATTCCGAAGT TCCTATTCTC TAGAAAGTAT 3181 AGGAACTTCC TTGGCCAAAA AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT 3241 CGAAAAGTTC GACAGCGTCT CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC 3301 TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG 3361 TTTCTACAAA GATCGTTATG TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA 3421 AGTGCTTGAC ATTGGGGAAT TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA 3481 GGGTGTCACG TTGCAAGACC TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC 3541 GGAGGCCATG GATGCGATCG CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT 3601 CGGACCGCAA GGAATCGGTC AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA 3661 TCCCCATGTG TATCACTGGC AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA 3721 GGCTCTCGAT GAGCTGATGC TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA 3781 CGCGGATTTC GGCTCCAACA ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA 3841 CTGGAGCGAG GCGATGTTCG GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG 3901 GCCGTGGTTG GCTTGTATGG AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT 3961 TGCAGGATCG CCGCGGCTCC GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA 4021 GAGCTTGGTT GACGGCAATT TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT 4081 CGTCCGATCC GGAGCCGGGA CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT 4141 CTGGACCGAT GGCTGTGTAG AAGTACTCGC CGATAGTGGA AACCGACGCC CCAGCACTCG 4201 TCCGAGGGCA AAGGAATAGC ACGTACTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA 4261 AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA 4321 TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA 4381 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG 4441 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA 4501 GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT 4561 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG 4621 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 4681 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC 4741 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC 4801 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 4861 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 4921 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 4981 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 5041 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 5101 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 5161 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 5221 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 5281 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 5341 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 5401 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 5461 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 5521 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 5581 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 5641 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 5701 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT 5761 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG 5821 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA 5881 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 5941 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 6001 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC 6061 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC 6121 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA 6181 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 6241 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 6301 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC 6361 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG 6421 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC 6481 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 6541 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 6601 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 6661 AGTGCCACCT GACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.