pUBE2T-Fluc-SV40-hRluc-Neo pla

pUBE2T-Fluc-SV40-hRluc-Neo plasmid

PVT109358

pUBE2T-Fluc-SV40-hRluc-Neo plasmid Information

Plasmid name: pUBE2T(human)-Fluc-SV40-hRluc-Neo
Alias: FANCT; PIG50; HSPC150
Gene lenght: 1500
Host: Mammalian cells
Use(s): Signal report,
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Fluc
Promoter: UBE2T
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: cer region-F1
Reverse primer:
Copy number: High
Induction:

pUBE2T-Fluc-SV40-hRluc-Neo plasmid Description

pUBE2T-Fluc-SV40-hRluc-Neo is a plasmid suitable for promoter detection in mammalian cells.

pUBE2T-Fluc-SV40-hRluc-Neo plasmid Sequence

1 CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC 61 CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCGGCCGCT TCGAGCAGAC ATGATAAGAT 121 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG 181 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 241 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG GTTTTTTTAA 301 GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA ACAAAATATT AACGCTTACA 361 ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGCG 421 GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT 481 CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 541 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 601 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 661 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 721 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC 781 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 841 GCTTGATTCT TCTGACACAA CAGTCTCGAA CCAAAGGCTG GAGCCACCAT GGCTTCCAAG 901 GTGTACGACC CCGAGCAACG CAAACGCATG ATCACTGGGC CTCAGTGGTG GGCTCGCTGC 961 AAGCAAATGA ACGTGCTGGA CTCCTTCATC AACTACTATG ATTCCGAGAA GCACGCCGAG 1021 AACGCCGTGA TTTTTCTGCA TGGTAACGCT GCCTCCAGCT ACCTGTGGAG GCACGTCGTG 1081 CCTCACATCG AGCCCGTGGC TAGATGCATC ATCCCTGATC TGATCGGAAT GGGTAAGTCC 1141 GGCAAGAGCG GGAATGGCTC ATATCGCCTC CTGGATCACT ACAAGTACCT CACCGCTTGG 1201 TTCGAGCTGC TGAACCTTCC AAAGAAAATC ATCTTTGTGG GCCACGACTG GGGGGCTTGT 1261 CTGGCCTTTC ACTACTCCTA CGAGCACCAA GACAAGATCA AGGCCATCGT CCATGCTGAG 1321 AGTGTCGTGG ACGTGATCGA GTCCTGGGAC GAGTGGCCTG ACATCGAGGA GGATATCGCC 1381 CTGATCAAGA GCGAAGAGGG CGAGAAAATG GTGCTTGAGA ATAACTTCTT CGTCGAGACC 1441 ATGCTCCCAA GCAAGATCAT GCGGAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG 1501 CCATTCAAGG AGAAGGGCGA GGTTAGACGG CCTACCCTCT CCTGGCCTCG CGAGATCCCT 1561 CTCGTTAAGG GAGGCAAGCC CGACGTCGTC CAGATTGTCC GCAACTACAA CGCCTACCTT 1621 CGGGCCAGCG ACGATCTGCC TAAGATGTTC ATCGAGTCCG ACCCTGGGTT CTTTTCCAAC 1681 GCTATTGTCG AGGGAGCTAA GAAGTTCCCT AACACCGAGT TCGTGAAGGT GAAGGGCCTC 1741 CACTTCAGCC AGGAGGACGC TCCAGATGAA ATGGGTAAGT ACATCAAGAG CTTCGTGGAG 1801 CGCGTGCTGA AGAACGAGCA GACCGGTGGT GGGAGCGGAG GTGGCGGATC AGGTGGCGGA 1861 GGCTCCGGAG GGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 1921 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 1981 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 2041 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 2101 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 2161 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 2221 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 2281 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 2341 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 2401 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 2461 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC 2521 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 2581 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 2641 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 2701 CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT 2761 GTTGGTTTTT TGTGTGAATC GATAGCGATA ATCTTTGCGC TTGCGTTTTC CCTTGTCCAG 2821 ATAGCCCAGT AGCTGACATT CATCCGGGGT CAGCACCGTT TCTGCGGACT GGCTTTCTAC 2881 GTAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT 2941 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA 3001 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 3061 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 3121 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 3181 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTC 3241 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 3301 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 3361 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 3421 ACTGCGGCCA ACTTACTTCT GACAACTATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 3481 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 3541 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA 3601 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 3661 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 3721 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 3781 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 3841 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ATTCGAAATG 3901 ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 3961 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 4021 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 4081 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 4141 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 4201 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 4261 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 4321 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 4381 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 4441 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 4501 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 4561 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 4621 GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 4681 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 4741 TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA 4801 AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG 4861 GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT 4921 GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG 4981 CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA 5041 AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTc gagctcaagc 5101 ttcgaattca ctgtagttgg tttattccta caactaaggt gaggaacccc aggggagcat 5161 cacagagtcc tagtgaacat gtggtgccct tgagagaaaa caccccacac aatggctatg 5221 gggaaccatt tgccacttaa caccagtttt gactagatgt agacatcact gcagtggcca 5281 aaattatact gatttacaag tttagaaaga gactgattat tactctgtta ccaataatag 5341 taataaatag tataatagta ataaataata gtaataataa taaattacta ttagtattac 5401 catcgttgtt ccaaagctac acagtgagct gagagcagtc agatgagggt tggctttttg 5461 cttgttttgt ttttccaccc acagcatttc caaaggctag tcaagttcca gccatgtgct 5521 ttagattaag tcaggtctgt gccatctttc agcgctcatg aagcattggc ctctggcaga 5581 ggcagctctg agaagttaag gttggcttaa ctataaacag gttcttcctt gttcagaaag 5641 taccatgcat ctagcgttgg aagatggttt aaaagtcatc tattccagct gggcacagtg 5701 gctcacgcct gtaatcccag cactttggga ggctgagaag gatggatcac ctgaggtcag 5761 gagttcaaga ccagcctggc caacacggtg aaaccccgtc tctactaaaa atacaaaaat 5821 tagctgggcg tggtggcgct tgcctgtaat cccagctatt cgggaggctg aggcaggaga 5881 actgcttgaa cccgggaggc agaggttgca gtgagccgag attgcaccat tgcactccag 5941 cctgggtgac agagcgagac tccgtctcaa aaaaaaaaaa aaaaaaaaaa agtcatctat 6001 tccaaactgc cacccagtgc aaggatcctt tctgcactat ccctaacaag tgatgaccta 6061 acttccactt gaacatttcc agtgatgaag gaattcacta ccctacctac tttgttcttc 6121 agagttctaa ttgatagaat gctttattga actaagttct gcatctctta gactttcatc 6181 cgttgtcctt aaatgtgtcc tctgaataaa cgtgtcctct gaagtagtaa aatctcccat 6241 gtgaaaaaaa ataaaggaag acagctatca cgtattctct tttccagatt aaatatttct 6301 ggtttctttc attgttcttg ctaagaaacg atttccagct ccttccttgg tctggcagag 6361 ccgactttgt aaccgaattt ggactgccgg atgacgtctc agggttcagc cgcagggtgt 6421 aggaagctga atggctgcgc ccagcgcgcg ctctcatttg aatgcgtgag cgttcagagc 6481 actggccaat agagaggagc ggtggctgcg cttctttccc ggtggattac tgtgggccaa 6541 tcacaatctt ccccagacag ggggcggagc cacgccaaca gtcagaggtc gcgcaggcgc 6601 tggtaccccg gatccCACCA TGGAAGATGC CAAAAACATT AAGAAGGGCC CAGCGCCATT 6661 CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC AAAGCCATGA AGCGCTACGC 6721 CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC GAGGTGGACA TTACCTACGC 6781 CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG AAGCGCTATG GGCTGAATAC 6841 AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG TTCTTCATGC CCGTGTTGGG 6901 TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC ATCTACAACG AGCGCGAGCT 6961 GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC GTGAGCAAGA AAGGGCTGCA 7021 AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA AAGATCATCA TCATGGATAG 7081 CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC GTGACTTCCC ATTTGCCACC 7141 CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC CGGGACAAAA CCATCGCCCT 7201 GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC GTAGCCCTAC CGCACCGCAC 7261 CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC GGCAACCAGA TCATCCCCGA 7321 CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC GGCATGTTCA CCACGCTGGG 7381 CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC TTCGAGGAGG AGCTATTCTT 7441 GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG GTGCCCACAC TATTTAGCTT 7501 CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC AACTTGCACG AGATCGCCAG 7561 CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG GCCAAACGCT TCCACCTACC 7621 AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC GCCATTCTGA TCACCCCCGA 7681 AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC TTCTTCGAGG CTAAGGTGGT 7741 GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC GGCGAGCTGT GCGTCCGTGG 7801 CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT ACAAACGCTC TCATCGACAA 7861 GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC GAGGACGAGC ACTTCTTCAT 7921 CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC CAGGTAGCCC CAGCCGAACT 7981 GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC GGGGTCGCCG GCCTGCCCGA 8041 CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG GAACACGGTA AAACCATGAC 8101 CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA ACCGCCAAGA AGCTGCGCGG 8161 TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC GGCAAGTTGG ACGCCCGCAA 8221 GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG ATCGCCGTGT AATTCTAGTT 8281 GTTTAAACGA GCTCGCTAGC CTCGAGTCTA GAGTCGACCT GCAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.