pCMV-UB-MCS-Hyg plasmid

pCMV-UB-MCS-Hyg plasmid

PVT109350

pCMV-UB-MCS-Hyg plasmid Information

Plasmid name: pCMV-UB-MCS-Hyg
Alias:
Gene lenght:
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-UB-MCS-Hyg plasmid Description

pCMV-UB-MCS-Hyg is a plasmid suitable for protein expression in mammalian cells.

pCMV-UB-MCS-Hyg plasmid Sequence

1 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 61 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat 121 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata 181 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa 241 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt 301 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt 361 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 421 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 481 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 541 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 601 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 661 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 721 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg 781 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca 841 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg 901 tgtacggtgg gaggtctata taagcagagc tctccctatc agtgatagag atctccctat 961 cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct ggagacgcca 1021 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc ggactctagc 1081 gtttaaactt aagatgaagg gaaacgacat cttcgaggcc cagaagatcg agtggcacga 1141 gggctccggc tccacctccg gctccggcat gcagatcttc gtgaagactc tgactggtaa 1201 gaccatcacc ctcgaggttg agcccagtga caccattgag aatgtcaagg caaagatcca 1261 agataaggaa ggcatccctc ctgaccagca gaggctgatc tttgctggaa aacagctgga 1321 agatgggcgc accctgtctg actacaacat ccagaaagag tccaccctgc acctggtact 1381 ccgtctcaga ggtgggtaat aaggatccac tagtccagtg tggtggaatt ctgcagatat 1441 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg 1501 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 1561 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 1621 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 1681 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 1741 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 1801 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 1861 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 1921 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 1981 cttgattagg gtgatggttc acgtacctag aagttcctat tccgaagttc ctattctcta 2041 gaaagtatag gaacttcctt ggccaaaaag cctgaactca ccgcgacgtc tgtcgagaag 2101 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa 2161 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc 2221 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg 2281 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc 2341 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag 2401 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc 2461 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg 2521 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc 2581 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac 2641 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg 2701 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc 2761 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat 2821 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa 2881 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc 2941 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc 3001 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc 3061 agcactcgtc cgagggcaaa ggaatagcac gtactacgag atttcgattc caccgccgcc 3121 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 3181 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat 3241 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 3301 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc 3361 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 3421 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 3481 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 3541 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 3601 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 3661 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 3721 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 3781 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 3841 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 3901 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 3961 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 4021 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 4081 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 4141 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 4201 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 4261 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 4321 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 4381 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 4441 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 4501 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 4561 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 4621 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 4681 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 4741 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 4801 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 4861 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 4921 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 4981 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggc

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.