pLV3-CMV-ONECUT3-3×FLAG-CopGFP

pLV3-CMV-ONECUT3-3×FLAG-CopGFP-Puro plasmid

PVT109342

pLV3-CMV-ONECUT3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ONECUT3(human)-3×FLAG-CopGFP-Puro
Alias: NM_001080488.2;OC3
Gene lenght: 1482
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ONECUT3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ONECUT3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ONECUT3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg agctgagcct ggagagcctg 2341 gggggcctgc acagcgtggc ccacgcgcag gcgggcgagc tgctgagccc gggccacgcg 2401 cgctcggcgg cggcgcagca ccgcggcctg gtggcgcccg ggcgcccggg cctggtggcc 2461 ggcatggcga gcctgctgga cggcggcggc ggcggcggcg gtgggggcgc cgggggcgcg 2521 ggcggcgcgg gcagcgcggg cggcggcgcg gacttccgcg gggaactggc gggcccgctg 2581 cacccggcaa tgggcatggc ctgcgaggcg ccgggcctgg gcggcaccta cacgacgctc 2641 acgcccctgc agcacctgcc gccgctcgcg gccgtggccg acaagttcca ccagcacgcg 2701 gcggccgcgg ccgtggccgg ggcgcacggc ggccatcccc acgcgcaccc gcacccggcg 2761 gccgcgccgc ccccgccacc cccgccgcag cgtctggcgg ccagcgtgag cggcagcttc 2821 accctcatgc gcgacgagcg ggcggcgctc gcctccgtgg gccacctcta cggaccctac 2881 ggcaaggagc tgcccgccat ggggtcgccg ctgtcgccgc tgcccaacgc gctgccgccc 2941 gcgctgcacg gcgccccgca gcccccgccg ccgccaccac ccccgccgct ggccgcctac 3001 ggcccgccag gccacctggc tggggacaag ctgctgccgc ccgccgcctt cgagccgcac 3061 gccgcgctgc tgggacgcgc ggaggacgca ctggcccgcg ggctgcccgg aggcggcggc 3121 ggcacaggca gcggcggagc gggcagcggg agcgccgcgg ggctgctggc gccgctgggc 3181 gggctggcgg cggccggggc gcacgggccg cacgggggag gcggcggccc cggcgggagc 3241 ggcggcggcc ccagcgcggg cgcagcggcc gaggagatca acaccaagga ggtggcgcag 3301 cgcatcacgg cggagctgaa gcgctacagc atcccgcagg caatcttcgc gcagcggatc 3361 ctgtgtcgct ctcagggcac gctctccgac ctgctgcgca accccaagcc gtggagcaag 3421 ctcaaatccg gccgcgagac cttccgcagg atgtggaagt ggctgcagga gccagagttc 3481 cagcgcatgt cggcgctgcg cttggcagcc tgcaagcgca aggaacagga gcagcagaag 3541 gagcgcgccc tgcagcccaa gaagcagcgc ctggtgttca ccgacctgca gcgacgcacg 3601 ctgatcgcca tcttcaagga gaacaagcgg ccgtccaagg agatgcaggt caccatctcg 3661 cagcagctcg gcttggagct caacaccgtc agcaacttct tcatgaacgc gcggcgccgc 3721 tgcatgaacc gctgggctga ggagcccagc acggcccccg ggggccccgc cggcgccacg 3781 gccactttct ccaaggccgg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 3841 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 3901 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 3961 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 4021 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 4081 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 4141 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 4201 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 4261 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 4321 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 4381 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 4441 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 4501 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 4561 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 4621 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 4681 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 4741 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 4801 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 4861 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 4921 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 4981 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 5041 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 5101 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 5161 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 5221 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 5281 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 5341 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 5401 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5461 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5521 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5581 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5641 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5701 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5761 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5821 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 5881 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5941 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 6001 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 6061 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 6121 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 6181 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6241 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 6301 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6361 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 6421 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 6481 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 6541 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6601 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6661 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6721 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6781 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 6841 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6901 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 6961 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 7021 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 7081 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 7141 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 7201 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 7261 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 7321 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 7381 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 7441 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 7501 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 7561 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 7621 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 7681 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 7741 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 7801 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 7861 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7921 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7981 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 8041 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 8101 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 8161 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 8221 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 8281 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 8341 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 8401 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 8461 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 8521 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 8581 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 8641 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 8701 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 8761 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 8821 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 8881 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8941 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 9001 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 9061 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 9121 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 9181 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 9241 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 9301 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 9361 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 9421 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 9481 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 9541 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 9601 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 9661 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 9721 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.