pCMV-AKAP1-3×HA-Neo plasmid

pCMV-AKAP1-3×HA-Neo plasmid

PVT109312

pCMV-AKAP1-3×HA-Neo plasmid Information

Plasmid name: pCMV-AKAP1(human)-3×HA-Neo
Alias: AKAP; AKAP121; AKAP149; AKAP84; D-AKAP1;PPP1R43; PRKA1; SAKAP84; TDRD17
Gene lenght: 2709
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-AKAP1-3×HA-Neo plasmid Description

pCMV-AKAP1-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-AKAP1-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatggc aatccagttc 721 cgttcgctct tccccttggc attgcctggg atgctggcgc tcctcggctg gtggtggttt 781 ttctctcgta aaaaaggcca tgtcagcagc catgatgagc agcaggtgga ggctggtgct 841 gtgcagctga gggctgaccc tgccatcaag gaacctctcc ccgtggaaga cgtctgtccc 901 aaagtagtgt ccacaccccc cagtgtcaca gagcctccag aaaaggaact gtccaccgtg 961 agcaagctgc ctgcagagcc cccagcattg ctccagacac acccaccttg ccgaagatca 1021 gagtcctcgg gcattcttcc taacaccaca gacatgagat tgcgaccagg aacacgcaga 1081 gatgacagta caaagctgga gctagccctg acaggtggtg aagccaaatc gattcctcta 1141 gagtgccccc tttcatcccc aaagggtgta ctattctcca gcaaatcagc tgaggtgtgt 1201 aagcaagatt cccccttcag cagggtgcca aggaaggtcc agccaggcta ccccgtagtc 1261 cccgcagaga agcgtagctc tggggagagg gcaagagaga caggtggggc cgaagggact 1321 ggtgatgccg tgttggggga aaaggtgctt gaagaagctc tgttgtctcg ggagcatgtc 1381 ttggaattgg agaacagcaa gggccccagc ctggcctctt tagaggggga agaagataag 1441 gggaagagca gctcatccca ggtggtgggg ccagtgcagg aggaagagta tgtagcagag 1501 aagttgccaa gtaggttcat cgagtcggct cacacagagc tggcaaagga cgatgcggcg 1561 ccagcacccc cagtcgcaga cgccaaagcc caggatagag gtgtcgaggg agaactgggc 1621 aatgaggaga gcttggatag aaatgaggag ggcttggata gaaatgagga gggcttggat 1681 agaaatgagg agagcttgga tagaaatgag gagggcttgg atagaaatga ggagattaag 1741 cgggctgcct tccagataat ctcccaagtg atctcagaag caaccgaaca ggtgctggcc 1801 accacggttg gcaaggttgc aggtcgtgtg tgtcaggcca gtcagctcca agggcagaag 1861 gaagagagct gtgtcccagt tcaccagaaa actgtcttgg gcccagacac tgcggagcct 1921 gccacagcag aggcagctgt tgccccgccg gatgctggcc tccccttgcc aggcctacca 1981 gcagagggct caccaccacc aaagacctac gtgagctgcc tgaagagcct tctgtccagc 2041 cccaccaagg acagtaagcc aaatatctct gcacaccaca tctccctggc ctcctgcctg 2101 gcactgacca cccccagtga agagttgccg gaccgggcag gcatcctggt ggaagatgcc 2161 acctgtgtca cctgcatgtc agacagcagc caaagtgtcc ctttggtggc ttctccagga 2221 cactgctcag attctttcag cacttcaggg cttgaagact cttgcacaga gaccagctcg 2281 agccccaggg acaaggccat caccccgcca ctgccagaaa gtactgtgcc cttcagcaat 2341 ggggtgctga agggggagtt gtcagacttg ggggctgagg atggatggac catggatgcg 2401 gaagcagatc attcaggagg ttctgacagg aacagcatgg attccgtgga tagctgttgc 2461 agtctcaaga agactgagag cttccaaaat gcccaggcag gctccaaccc taagaaggtc 2521 gacctcatca tctgggagat cgaggtgcca aagcacttag tcggtcggct aattggcaag 2581 caggggcgct atgtgagttt tctgaagcaa acatctggtg ccaagatcta catttcaacc 2641 ctgccttaca cccagagcgt ccagatctgc cacatagaag gctctcaaca tcatgtagac 2701 aaagcgctga acttgattgg gaagaagttc aaagagctga acctcaccaa tatctacgct 2761 cccccattgc cttcactggc actgccttct ctgccgatga catcctggct catgctgcct 2821 gatggcatca ccgtggaggt cattgtggtc aaccaggtca atgccgggca cctgttcgtg 2881 cagcagcaca cacaccctac cttccacgcg ctgcgcagcc tcgaccagca gatgtacctc 2941 tgttactctc agcctggaat ccccaccttg cccaccccag tggaaataac ggtcatctgt 3001 gccgcccctg gtgcggacgg ggcctggtgg cgagcccaag tggttgcctc ctacgaggag 3061 accaacgaag tggagattcg atacgtggac tacggcggat ataagagggt gaaagtagac 3121 gtgctccggc aaatcaggtc tgactttgtc accctgccgt ttcagggagc agaagtcctt 3181 ctggacagtg tgatgcccct gtcagacgat gaccagtttt caccggaagc agatgccgcc 3241 atgagcgaga tgacggggaa tacagcactg cttgctcagg tgacaagtta cagtccaact 3301 ggtcttcctc tgattcagct gtggagtgtg gttggagatg aagtggtgtt gataaaccgg 3361 tccctggtgg agcgaggcct tgcccagtgg gtagacagct actacacaag ccttCTCGAG 3421 TACCCATACG ACGTACCTGA TTATGCAACT TATCCTTATG ATGTACCTGA TTATGCTAGC 3481 TATCCTTATG ATGTCCCAGA CTACGCATAG TGAGGGCCCG ATATCTCGAC AATCAACCTC 3541 TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC 3601 TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA 3661 TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG 3721 TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA 3781 TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG 3841 CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG 3901 ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG 3961 CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG 4021 ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC 4081 CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGAAACGGG GGAGGCTAAC 4141 TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT ATGACGGCAA TAAAAAGACA 4201 GAATAAAACG CACGGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT 4261 GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT 4321 TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG 4381 CGGCAGGCCC TGCCATAGCA GATCTGCGCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC 4441 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 4501 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 4561 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GCATCCCTTT AGGGTTCCGA TTTAGTGCTT 4621 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 4681 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 4741 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 4801 TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 4861 ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 4921 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG 4981 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC 5041 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA 5101 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT 5161 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC 5221 TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA 5281 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA 5341 CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG 5401 GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA 5461 GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT 5521 TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT 5581 GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT 5641 GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG 5701 AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA 5761 GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA 5821 TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT 5881 TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT 5941 GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT 6001 TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT 6061 CTTCTGAGCG GGACTCTGGG GTTCGCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 6121 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 6181 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 6241 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 6301 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 6361 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 6421 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 6481 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 6541 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 6601 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 6661 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 6721 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 6781 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 6841 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 6901 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 6961 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT 7021 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 7081 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 7141 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 7201 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG 7261 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 7321 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 7381 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 7441 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 7501 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 7561 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 7621 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 7681 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 7741 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 7801 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 7861 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 7921 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 7981 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 8041 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 8101 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 8161 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 8221 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 8281 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 8341 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 8401 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 8461 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 8521 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG 8581 GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT 8641 AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT 8701 TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.