pCMV-KDM6B-3×FLAG-Blast plasmi

pCMV-KDM6B-3×FLAG-Blast plasmid

PVT109233

pCMV-KDM6B-3×FLAG-Blast plasmid Information

Plasmid name: pCMV-KDM6B(human)-3×FLAG-Blast
Alias: NM_001080424.2;JMJD3; NEDCFSA; NEDSST
Gene lenght: 5046
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-KDM6B-3×FLAG-Blast plasmid Description

pCMV-KDM6B-3×FLAG-Blast is a plasmid suitable for protein expression in mammalian cells.

pCMV-KDM6B-3×FLAG-Blast plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 AAGCTTTCTA GAGAATTCga tatcGGATCC GCCACCatgc atcgggcagt ggaccctcca 961 ggggcccgcg ctgcacggga agcctttgcc cttgggggcc tgagctgtgc tggggcctgg 1021 agctcctgcc cgcctcatcc ccctcctcgt agcgcatggc tgcctggagg cagatgctca 1081 gccagcattg ggcagccccc gcttcctgct cccctacccc cttcacatgg cagtagttct 1141 gggcacccca gcaaaccata ttatgctcca ggggcgccca ctccaagacc cctccatggg 1201 aagctggaat ccctgcatgg ctgtgtgcag gcattgctcc gggagccagc ccagccaggg 1261 ctttgggaac agcttgggca actgtacgag tcagagcacg atagtgagga ggccacacgc 1321 tgctaccaca gcgcccttcg atacggagga agcttcgctg agctggggcc ccgcattggc 1381 cgactgcagc aggcccagct ctggaacttt catactggct cctgccagca ccgagccaag 1441 gtcctgcccc cactggagca agtgtggaac ttgctacacc ttgagcacaa acggaactat 1501 ggagccaagc ggggaggtcc cccggtgaag cgagctgctg aacccccagt ggtgcagcct 1561 gtgcctcctg cagcactctc aggcccctca ggggaggagg gcctcagccc tggaggcaag 1621 cgaaggagag gctgcaactc tgaacagact ggccttcccc cagggctgcc actgcctcca 1681 ccaccattac caccaccacc accaccacca ccaccaccac caccacccct gcctggcctg 1741 gctaccagcc ccccatttca gctaaccaag ccagggctgt ggagtaccct gcatggagat 1801 gcctggggcc cagagcgcaa gggttcagca cccccagagc gccaggagca gcggcactcg 1861 ctgcctcacc catatccata cccagctcca gcgtacaccg cgcacccccc tggccaccgg 1921 ctggtcccgg ctgctccccc aggcccaggc ccccgccccc caggagcaga gagccatggc 1981 tgcctgcctg ccacccgtcc ccccggaagt gaccttagag agagcagagt tcagaggtcg 2041 cggatggact ccagcgtttc accagcagca accaccgcct gcgtgcctta cgccccttcc 2101 cggccccctg gcctccccgg caccaccacc agcagcagca gtagcagcag cagcaacact 2161 ggtctccggg gcgtggagcc gaacccaggc attcccggcg ctgaccatta ccaaactccc 2221 gcgctggagg tctctcacca tggccgcctg gggccctcgg cacacagcag tcggaaaccg 2281 ttcttggggg ctcccgctgc cactccccac ctatccctgc cacctggacc ttcctcaccc 2341 cctccacccc cctgtccccg cctcttacgc cccccaccac cccctgcctg gttgaagggt 2401 ccggcctgcc gggcagcccg agaggatgga gagatcttag aagagctctt ctttgggact 2461 gagggacccc cccgccctgc cccaccaccc ctcccccatc gcgagggctt cttggggcct 2521 ccggcctccc gcttttctgt gggcactcag gattctcaca cccctcccac tcccccaacc 2581 ccaaccacca gcagtagcaa cagcaacagt ggcagccaca gcagcagccc tgctgggcct 2641 gtgtcctttc ccccaccacc ctatctggcc agaagtatag acccccttcc ccggcctccc 2701 agcccagcac agaaccccca ggacccacct cttgtacccc tgactcttgc cctgcctcca 2761 gcccctcctt cctcctgcca ccaaaatacc tcaggaagct tcaggcgccc ggagagcccc 2821 cggcccaggg tctccttccc aaagaccccc gaggtggggc cggggccacc cccaggcccc 2881 ctgagtaaag ccccccagcc tgtgccgccc ggggttgggg agctgcctgc ccgaggccct 2941 cgactctttg attttccccc cactccgctg gaggaccagt ttgaggagcc agccgaattc 3001 aagatcctac ctgatgggct ggccaacatc atgaagatgc tggacgaatc cattcgcaag 3061 gaagaggaac agcaacaaca cgaagcaggc gtggcccccc aacccccgct gaaggagccc 3121 tttgcatctc tgcagtctcc tttccccacc gacacagccc ccaccactac tgctcctgct 3181 gtcgccgtca ccaccaccac caccaccacc accaccacca cggccaccca ggaagaggag 3241 aagaagccac caccagccct accaccacca ccgcctctag ccaagttccc tccaccctct 3301 cagccacagc caccaccacc cccacccccc agcccggcca gcctgctcaa atccttggcc 3361 tccgtgctgg agggacaaaa gtactgttat cgggggactg gagcagctgt ttccacccgg 3421 cctgggccct tgcccaccac tcagtattcc cctggccccc catcaggtgc taccgccctg 3481 ccgcccacct cagcggcccc tagcgcccag ggctccccac agccctctgc ttcctcgtca 3541 tctcagttct ctacctcagg cgggccctgg gcccgggagc gcagggcggg cgaagagcca 3601 gtcccgggcc ccatgacccc cacccaaccg cccccacccc tatctctgcc ccctgctcgc 3661 tctgagtctg aggtgctaga agagatcagc cgggcttgcg agacccttgt ggagcgggtg 3721 ggccggagtg ccactgaccc agccgaccca gtggacacag cagagccagc ggacagtggg 3781 actgagcgac tgctgccccc cgcacaggcc aaggaggagg ctggcggggt ggcggcagtg 3841 tcaggcagct gtaagcggcg acagaaggag catcagaagg agcatcggcg gcacaggcgg 3901 gcctgtaagg acagtgtggg tcgtcggccc cgtgagggca gggcaaaggc caaggccaag 3961 gtccccaaag aaaagagccg ccgggtgctg gggaacctgg acctgcagag cgaggagatc 4021 cagggtcgtg agaagtcccg gcccgatctt ggcggggcct ccaaggccaa gccacccaca 4081 gctccagccc ctccatcagc tcctgcacct tctgcccagc ccacaccccc gtcagcctct 4141 gtccctggaa agaaggctcg ggaggaagcc ccagggccac cgggtgtcag ccgggccgac 4201 atgctgaagc tgcgctcact tagtgagggg ccccccaagg agctgaagat ccggctcatc 4261 aaggtagaga gtggtgacaa ggagaccttt atcgcctctg aggtggaaga gcggcggctg 4321 cgcatggcag acctcaccat cagccactgt gctgctgacg tcgtgcgcgc cagcaggaat 4381 gccaaggtga aagggaagtt tcgagagtcc tacctttccc ctgcccagtc tgtgaaaccg 4441 aagatcaaca ctgaggagaa gctgccccgg gaaaaactca acccccctac acccagcatc 4501 tatctggaga gcaaacggga tgccttctca cctgtcctgc tgcagttctg tacagaccct 4561 cgaaatccca tcacagtgat ccggggcctg gcgggctccc tgcggctcaa cttgggcctc 4621 ttctccacca agaccctggt ggaagcgagt ggcgaacaca ccgtggaagt tcgcacccag 4681 gtgcagcagc cctcagatga gaactgggat ctgacaggca ctcggcagat ctggccttgt 4741 gagagctccc gttcccacac caccattgcc aagtacgcac agtaccaggc ctcatccttc 4801 caggagtctc tgcaggagga gaaggagagt gaggatgagg agtcagagga gccagacagc 4861 accactggaa cccctcctag cagcgcacca gacccgaaga accatcacat catcaagttt 4921 ggcaccaaca tcgacttgtc tgatgctaag cggtggaagc cccagctgca ggagctgctg 4981 aagctgcccg ccttcatgcg ggtaacatcc acgggcaaca tgctgagcca cgtgggccac 5041 accatcctgg gcatgaacac ggtgcagctg tacatgaagg tgcccggcag ccgaacgcca 5101 ggccaccagg agaataacaa cttctgctcc gtcaacatca acattggccc aggcgactgc 5161 gagtggttcg cggtgcacga gcactactgg gagaccatca gcgctttctg tgatcggcac 5221 ggcgtggact acttgacggg ttcctggtgg ccaatcctgg atgatctcta tgcatccaat 5281 attcctgtgt accgcttcgt gcagcgaccc ggagacctcg tgtggattaa tgcggggact 5341 gtgcactggg tgcaggccac cggctggtgc aacaacattg cctggaacgt ggggcccctc 5401 accgcctatc agtaccagct ggccctggaa cgatacgagt ggaatgaggt gaagaacgtc 5461 aaatccatcg tgcccatgat tcacgtgtca tggaacgtgg ctcgcacggt caaaatcagc 5521 gaccccgact tgttcaagat gatcaagttc tgcctgctgc agtccatgaa gcactgccag 5581 gtgcaacgcg agagcctggt gcgggcaggg aagaaaatcg cttaccaggg ccgtgtcaag 5641 gacgagccag cctactactg caacgagtgc gatgtggagg tgtttaacat cctgttcgtg 5701 acaagtgaga atggcagccg caacacgtac ctggtacact gcgagggctg tgcccggcgc 5761 cgcagcgcag gcctgcaggg cgtggtggtg ctggagcagt accgcactga ggagctggct 5821 caggcctacg acgccttcac gctggtgagg gcccggcggg cgcgcgggca gcggaggagg 5881 gcactggggc aggctgcagg gacgggcttc gggagcccgg ccgcgccttt ccctgagccc 5941 ccgccggctt tctcccccca ggccccagcc agcacgtcgc gaCTCGAGAC CGGTGACTAC 6001 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG 6061 TGACCGGTaA GTTTAAACCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT 6121 GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT 6181 TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG 6241 GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG 6301 GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT 6361 CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 6421 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 6481 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 6541 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 6601 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 6661 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 6721 TTATAAGGGA TTTTGGCCAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 6781 TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT 6841 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA 6901 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 6961 CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT 7021 CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT 7081 CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC 7141 TCCCgggATG GCCAAGCCTT TGTCTCAAGA AGAATCCACC CTCATTGAAA GAGCAACGGC 7201 TACAATCAAC AGCATCCCCA TCTCTGAAGA CTACAGCGTC GCCAGCGCAG CTCTCTCTAG 7261 CGACGGCCGC ATCTTCACTG GTGTCAATGT ATATCATTTT ACTGGGGGAC CTTGTGCAGA 7321 ACTCGTGGTG CTGGGCACTG CTGCTGCTGC GGCAGCTGGC AACCTGACTT GTATCGTCGC 7381 GATCGGAAAT GAGAACAGGG GCATCTTGAG CCCCTGCGGA CGGTGCCGAC AGGTGCTTCT 7441 CGATCTGCAT CCTGGGATCA AAGCCATAGT GAAGGACAGT GATGGACAGC CGACGGCAGT 7501 TGGGATTCGT GAATTGCTGC CCTCTGGTTA TGTGTGGGAG GGCTAATTCG AAATGACCGA 7561 CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG 7621 GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT 7681 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA 7741 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG 7801 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG 7861 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC 7921 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA 7981 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA 8041 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC 8101 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC 8161 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 8221 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 8281 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 8341 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 8401 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 8461 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 8521 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 8581 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 8641 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 8701 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 8761 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT 8821 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 8881 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 8941 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 9001 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 9061 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 9121 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 9181 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 9241 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 9301 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 9361 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 9421 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 9481 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 9541 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 9601 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 9661 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 9721 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 9781 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 9841 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 9901 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 9961 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 10021 ACCTGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.