pRCC-SNR52-SAH1-sgRNA2-Cas9 pl

pRCC-SNR52-SAH1-sgRNA2-Cas9 plasmid

PVT108179

pRCC-SNR52-SAH1-sgRNA2-Cas9 plasmid Information

Plasmid name: pRCC-SNR52-SAH1(yeast)-sgRNA2-Cas9
Alias: TACGGGCGCCTTTCAAAGGT
Gene lenght:
Host: Yeast
Use(s): Gene knockout
Fragment type: CRISPR
Species: Yeast
Prokaryotic resistance: Amp
Screening marker: Nrs
Fluorescent labeling:
Promoter: SNR52
Replicon: pUC,2U
Growth Strain(s): Stbl3
Growth Temperature: 30°C
Vector backbone:
Forward primer: Forward left ITR
Reverse primer:
Copy number: High
Induction:

pRCC-SNR52-SAH1-sgRNA2-Cas9 plasmid Description

pRCC-SNR52-SAH1-sgRNA2-Cas9 This plasmid is suitable for gene knockout in yeast.

pRCC-SNR52-SAH1-sgRNA2-Cas9 plasmid Sequence

1 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC 61 ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT 121 GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG 181 ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC 241 TTAGTATGAT CCAATATCAA AGGAAATGAT AGCATTGAAG GATGAGACTA ATCCAATTGA 301 GGAGTGGCAG CATATAGAAC AGCTAAAGGG TAGTGCTGAA GGAAGCATAC GATACCCCGC 361 ATGGAATGGG ATAATATCAC AGGAGGTACT AGACTACCTT TCATCCTACA TAAATAGACG 421 CATATAAGTA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT GTATATATAT 481 ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT GCGCAGCTCG 541 CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT TCCGAAGTTC 601 CTATTCTCTA GAAAGTATAG GAACTTCAGA GCGCTTTTGA AAACCAAAAG CGCTCTGAAG 661 ACGCACTTTC AAAAAACCAA AAACGCACCG GACTGTAACG AGCTACTAAA ATATTGCGAA 721 TACCGCTTCC ACAAACATTG CTCAAAAGTA TCTCTTTGCT ATATATCTCT GTGCTATATC 781 CCTATATAAC CTACCCATCC ACCTTTCGCT CCTTGAACTT GCATCTAAAC TCGACCTCTA 841 CATTTTTTAT GTTTATCTCT AGTATTACTC TTTAGACAAA AAAATTGTAG TAAGAACTAT 901 TCATAGAGTG AATCGAAAAC AATACGAAAA TGTAAACATT TCCTATACGT AGTATATAGA 961 GACAAAATAG AAGAAACCGT TCATAATTTT CTGACCAATG AAGAATCATC AACGCTATCA 1021 CTTTCTGTTC ACAAAGTATG CGCAATCCAC ATCGGTATAG AATATAATCG GGGATGCCTT 1081 TATCTTGAAA AAATGCACCC GCAGCTTCGC TAGTAATCAG TAAACGCGGG AAGTGGAGTC 1141 AGGCTTTTTT TATGGAAGAG AAAATAGACA CCAAAGTAGC CTTCTTCTAA CCTTAACGGA 1201 CCTACAGTGC AAAAAGTTAT CAAGAGACTG CATTATAGAG CGCACAAAGG AGAAAAAAAG 1261 TAATCTAAGA TGCTTTGTTA GAAAAATAGC GCTCTCGGGA TGCATTTTTG TAGAACAAAA 1321 AAGAAGTATA GATTCTTTGT TGGTAAAATA GCGCTCTCGC GTTGCATTTC TGTTCTGTAA 1381 AAATGCAGCT CAGATTCTTT GTTTGAAAAA TTAGCGCTCT CGCGTTGCAT TTTTGTTTTA 1441 CAAAAATGAA GCACAGATTC TTCGTTGGTA AAATAGCGCT TTCGCGTTGC ATTTCTGTTC 1501 TGTAAAAATG CAGCTCAGAT TCTTTGTTTG AAAAATTAGC GCTCTCGCGT TGCATTTTTG 1561 TTCTACAAAA TGAAGCACAG ATGCTTCGTT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG 1621 GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT 1681 AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC 1741 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA 1801 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC 1861 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA 1921 TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG 1981 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA 2041 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA 2101 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA 2161 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC 2221 TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA 2281 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG 2341 ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT 2401 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC 2461 TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA 2521 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT 2581 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT 2641 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG 2701 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC 2761 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG 2821 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG 2881 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT 2941 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG 3001 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC 3061 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG 3121 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG 3181 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG 3241 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC 3301 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGGCT 3361 AGGGATAACA GGGTAATTCT TTGAAAAGAT AATGTATGAT TATGCTTTCA CTCATATTTA 3421 TACAGAAACT TGATGTTTTC TTTCGAGTAT ATACAAGGTG ATTACATGTA CGTTTGAAGT 3481 ACAACTCTAG ATTTTGTAGT GCCCTCTTGG GCTAGCGGTA AAGGTGCGCA TTTTTTCACA 3541 CCCTACAATG TTCTGTTCAA AAGATTTTGG TCAAACGCTG TAGAAGTGAA AGTTGGTGCG 3601 CATGTTTCGG CGTTCGAAAC TTCTCCGCAG TGAAAGATAA ATGATCGGAT CCGTACGGGC 3661 GCCTTTCAAA GGTGCGGCCG CGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT 3721 CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGGTGC TTTTTTTGTT TTTTATGTCT 3781 CGGCGAATTG GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC CTTCTCAAGC 3841 AAGGTTTTCA GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA AAGAAAAATT 3901 TGAAATATAA ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT AGGGACCTAG 3961 ACTTCAGGTT GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG AGGGCGTGAA 4021 TGTAAGCGTG ACATAACTAA TTACATGACT CGAGGTCGAC TTAAACCTTT CTCTTCTTCT 4081 TTGGGTCAGC TCTAGAGTCA CCACCCAATT GAGACAAGTC AATTCTGGTT TCGTACAAAC 4141 CGGTAATAGA TTGGTGAATC AAGGTAGCGT CCAAAACTTC CTTGGTAGAG GTGTATCTCT 4201 TTCTGTCAAT GGTGGTGTCG AAGTACTTGA AAGCAGCTGG AGCACCCAAG TTGGTCAAGG 4261 TGAACAAGTG AATAATGTTT TCAGCTTGTT CTCTAATTGG CTTGTCTCTG TGCTTGTTGT 4321 AAGCAGACAA AACCTTGTCC AAGTTAGCGT CAGCCAAAAT AACTCTCTTA GAGAATTCAG 4381 AAATTTGTTC AATAATTTCG TCCAAGTAGT GCTTGTGTTG TTCAACGAAC AATTGCTTTT 4441 GTTCGTTGTC TTCTGGAGAA CCCTTCAACT TTTCGTAGTG AGAAGCCAAG TACAAGAAGT 4501 TAACGTACTT AGATGGCAAA GCCAATTCGT TACCCTTTTG CAATTCACCA GCAGAAGCCA 4561 ACATTCTCTT TCTACCGTTT TCCAATTCGA ACAAAGAGTA CTTTGGCAAC TTAATAATCA 4621 AGTCCTTCTT AACTTCCTTG TAACCCTTAG CTTCCAAGAA GTCAATTGGG TTCTTTTCGA 4681 AAGAAGATCT TTCCATAATG GTAATACCCA ACAATTCCTT AACAGACTTC AACTTCTTAG 4741 ACTTACCCTT TTCAACCTTA GCAACAACCA AAACAGAGTA AGCAACGGTT GGAGAGTCGA 4801 AACCACCGTA CTTCTTTGGG TCCCAGTCCT TCTTTCTAGC AATCAACTTG TCAGAGTTTC 4861 TCTTTGGCAA AATAGATTCC TTAGAGAAAC CACCGGTTTG AACTTCGGTC TTCTTAACAA 4921 TGTTAACTTG TGGCATAGAC AAAACCTTTC TAACGGTAGC GAAGTCTCTA CCCTTGTCCC 4981 AAACAATTTC ACCGGTTTCA CCGTTGGTTT CAATCAATGG TCTCTTTCTA ATTTCACCGT 5041 TAGCCAAGGT AATTTCGGTC TTGAAGAAGT TCATAATGTT AGAGTAGAAG AAGTACTTAG 5101 CGGTAGCCTT ACCAATTTCT TGTTCAGACT TAGCAATCAT CTTTCTAACG TCGTAAACCT 5161 TGTAGTCACC GTAAACGAAT TCAGATTCCA ACTTTGGGTA CTTCTTAATC AAAGCGGTAC 5221 CAACAACAGC GTTCAAGTAA GCGTCGTGAG CGTGGTGGTA GTTGTTAATT TCTCTAACCT 5281 TGTAGAATTG GAAGTCCTTT CTGAAGTCAG AAACCAACTT AGACTTCAAG GTAATAACCT 5341 TAACTTCTCT AATCAACTTG TCGTTTTCGT CGTACTTGGT GTTCATTCTA GAGTCCAAAA 5401 TTTGAGCAAC GTGCTTGGTA ATTTGTCTGG TTTCAACCAA TTGTCTCTTA ATGAAACCAG 5461 CCTTGTCCAA TTCAGACAAA CCACCTCTTT CAGCCTTGGT CAAGTTGTCG AACTTTCTTT 5521 GGGTAATCAA CTTAGCGTTC AACAATTGTC TCCAGTAGTT CTTCATCTTC TTAACAACTT 5581 CTTCAGATGG AACGTTGTCA GACTTACCTC TGTTCTTGTC AGATCTGGTC AAAACCTTGT 5641 TGTCAATAGA GTCGTCCTTC AAGAAAGATT GTGGAACAAT GTGGTCAACG TCGTAGTCAG 5701 ACAATCTGTT AATGTCCAAT TCTTGGTCAA CGTACATGTC TCTACCGTTT TGCAAGTAGT 5761 ACAAGTACAA CTTTTCGTTT TGCAATTGGG TGTTTTCAAC TGGGTGTTCC TTCAAAATTT 5821 GAGAACCCAA TTCCTTAATA CCTTCTTCAA TTCTCTTCAT TCTTTCTCTA GAGTTCTTTT 5881 GACCCTTTTG GGTGGTTTGG TTTTCTCTAG CCATTTCAAT AACAATGTTT TCTGGCTTGT 5941 GTCTACCCAT AACCTTAACC AATTCGTCAA CAACCTTAAC GGTTTGCAAA ATACCCTTCT 6001 TAATAGCTGG AGAACCAGCC AAGTTAGCAA TGTGTTCGTG CAAAGAGTCA CCTTGACCAG 6061 AAACTTGAGC CTTTTGAATG TCTTCCTTGA AGGTCAAAGA GTCGTCGTGA ATCAATTGCA 6121 TGAAGTTTCT GTTAGCGAAA CCGTCAGACT TCAAGAAGTC CAAAATGGTC TTACCAGATT 6181 GCTTGTCTCT AATACCGTTA ATCAACTTTC TAGACAATCT ACCCCAACCG GTGTATCTTC 6241 TTCTCTTCAA TTGCTTCATA ACCTTGTCGT CGAACAAGTG AGCGTAGGTC TTCAATCTTT 6301 CTTCAATCAT TTCTCTGTCT TCGAACAAGG TCAAGGTCAA AACAATGTCT TCCAAAATGT 6361 CTTCGTTTTC TTCGTTGTCC AAGAAGTCCT TGTCCTTAAT AATCTTCAAC AAGTCGTGGT 6421 AGGTACCCAA AGAAGCGTTG AATCTGTCTT CAACACCAGA AATTTCAACA GAGTCGAAAC 6481 ATTCAATCTT CTTGAAGTAG TCTTCCTTCA ATTGCTTAAC GGTAACCTTT CTGTTGGTCT 6541 TGAACAACAA GTCAACAATA GCCTTCTTTT GTTCACCAGA CAAGAAAGCT GGCTTTCTCA 6601 TACCTTCGGT AACGTACTTA ACCTTGGTCA ATTCGTTGTA AACGGTGAAG TATTCGTACA 6661 ACAAAGAGTG CTTTGGCAAA ACCTTTTCGT TTGGCAAGTT CTTGTCGAAG TTGGTCATTC 6721 TTTCAATGAA AGATTGAGCA GAAGCACCCT TGTCAACAAC TTCTTCGAAG TTCCATGGGG 6781 TAATGGTTTC TTCAGACTTT CTGGTCATCC AAGCGAATCT AGAGTTACCT CTAGCCAATG 6841 GACCAACGTA GTATGGAATT CTGAAGGTCA AAATCTTTTC AATCTTTTCT CTGTTGTCCT 6901 TCAAGAATGG GTAGAAGTCT TCTTGTCTTC TCAAAATAGC GTGCAATTCA CCCAAGTGAA 6961 TTTGGTGTGG AATAGAACCG TTGTCGAAGG TTCTTTGCTT TCTCAACAAG TCTTCTCTGT 7021 TCAACTTAAC CAACAATTCT TCGGTACCGT CCATCTTTTC CAAAATTGGC TTAATGAACT 7081 TGTAGAATTC TTCTTGAGAA GCACCACCGT CAATGTAACC AGCGTAACCG TTCTTAGATT 7141 GGTCGAAGAA AATTTCCTTG TACTTTTCTG GCAATTGTTG TCTAACCAAA GCCTTCAACA 7201 AGGTCAAGTC TTGGTGGTGT TCGTCGTATC TCTTAATCAT AGAAGCAGAC AATGGAGCCT 7261 TGGTAATTTC GGTGTTAACT CTCAAAATGT CAGACAACAA AATAGCGTCA GACAAGTTCT 7321 TAGCAGCCAA GAACAAGTCA GCGTATTGGT CACCAATTTG AGCCAACAAG TTGTCCAAGT 7381 CGTCGTCGTA GGTGTCCTTA GACAATTGCA ACTTAGCGTC TTCAGCCAAG TCGAAGTTAG 7441 ACTTGAAGTT TGGGGTCAAA CCCAAAGACA AAGCAATCAA GTTACCGAAC AAACCGTTCT 7501 TCTTTTCACC TGGCAATTGA GCAATCAAGT TTTCCAATCT TCTAGACTTA GACAATCTAG 7561 CAGACAAAAT AGCCTTAGCG TCAACACCAG AAGCGTTAAT TGGGTTTTCT TCGAACAATT 7621 GGTTGTAGGT TTGAACCAAT TGAATGAACA ACTTGTCAAC GTCAGAGTTG TCTGGGTTCA 7681 AGTCACCTTC AATCAAGAAG TGACCTCTGA ACTTAATCAT GTGAGCCAAA GCCAAGTAAA 7741 TCAATCTCAA GTCAGCCTTG TCGGTAGAGT CAACCAACTT CTTTCTCAAG TGGTAAATGG 7801 TTGGGTACTT TTCGTGGTAA GCAACTTCGT CAACAATGTT ACCGAAAATT GGGTGTCTTT 7861 CGTGCTTCTT GTCTTCTTCA ACCAAGAAAG ATTCTTCCAA TCTGTGGAAG AAAGAGTCGT 7921 CAACCTTAGC CATTTCGTTA GAGAAAATTT CTTGCAAGTA ACAAATTCTG TTCTTTCTTC 7981 TGGTGTATCT TCTTCTAGCG GTTCTCTTCA ATCTGGTAGC TTCAGCGGTT TCACCAGAGT 8041 CGAACAACAA AGCACCAATC AAGTTCTTCT TAATAGAGTG TCTGTCGGTG TTACCCAAAA 8101 CCTTGAACTT CTTAGATGGA ACCTTGTATT CGTCGGTAAT AACAGCCCAA CCAACAGAGT 8161 TGGTACCAAT GTCCAAACCA ATAGAGTACT TCTTGTCCAT ATGTATTATC GTATTCCTTT 8221 TTCGTTCACT TACTATTGCT ATTCGCACTC ACTACAGATC TATATTTTAT GTTCCACCCT 8281 GTTGTCAGAT CAGGAAAGGA ATGAAGAGGC TCTATTTAAA CCAAATACAC CTCTTCGTTT 8341 CTCAATATTG TTGGGCTTTC CTTTTTTTTT TCCTTCCACG TCTATCTATT GTCTCTATCA 8401 AAATTAGGAA AAAGAAATGA AGAACGATGA GCCAAACGAG GCTGAGACAA TCATGCATCC 8461 GTACATTCTA TGTATGTTTG GGTGTATCTT TCCAGATTCG GTTCTTTTTT TTTTTCAGTC 8521 CAATTGAAAA AAAAAAGGAA CAGGAACAAC GGGTCGAAAT TTGCCGTCAC ATTCAGAGCG 8581 AAGAGCGACA GGGGAATTGG TGGGCTAACC ACCTGTTGAG CCCAGCTAGC AGCTGTCGGT 8641 AAACCGTCGT TGGGCGGGGC GCGACAATGC AGCCAGCGGA GGCATTGTAT GGTTTCCGTC 8701 TGGAGAGTGG GCTTAGCCGG CCTGGCACTC CTTCTCTAAG CAGAGTGAGA AGGGAATCTT 8761 TTTACTGGAG AAGAGTGTTT GAATCCAGCA GAAGGTAATA CGCTGCGGTA TTTTCTCCTT 8821 ACGCATCTGT GCGGTATTTC ACACCGCGTT TTCGACACTG GATGGCGGCG TTAGTATCGA 8881 ATCGACAGCA GTATAGCGAC CAGCATTCAC ATACGATTGA CGCATGATAT TACTTTCTGC 8941 GCACTTAACT TCGCATCTGG GCAGATGATG TCGAGGCGAA AAAAAATATA AATCACGCTA 9001 ACATTTGATT AAAATAGAAC AACTACAATA TAAAAAAACT ATACAAATGA CAAGTTCTTG 9061 AAAACAAGAA TCTTTTTATT GTCAGTACTG ATTAGGGGCA GGGCATGCTC ATGTAGAGCG 9121 CCTGCTCGCC GTCCGAGGCG GTGCCGTCGT ACAGGGCGGT GTCCAGGCCG CAGAGGGTGA 9181 ACCCCATCCG CCGGTACGCG TGGATCGCCG GTGCGTTGAC GTTGGTGACC TCCAGCCAGA 9241 GGTGCCCGGC GCCCCGCTCG CGGGCGAACT CCGTCGCGAG CCCCATCAAC GCGCGCCCGA 9301 CCCCGTGCCC CCGGTGCTCC GGGGCGACCT CGATGTCCTC GACGGTCAGC CGGCGGTTCC 9361 AGCCGGAGTA CGAGACGACC ACGAAGCCCG CCAGGTCGCC GTCGTCCCCG TACGCGACGA 9421 ACGTCCGGGA GTCCGGGTCG CCGTCCTCCC CGTCGTCCGA TTCGTCGTCC GATTCGTCGT 9481 CGGGGAACAC CTTGGTCAGG GGCGGGTCCA CCGGCACCTC CCGCAGGGTG AAGCCGTCCC 9541 CGGTGGCGGT GACGCGGAAG ACGGTGTCGG TGGTGAAGGA CCCATCCAGT GCCTCGATGG 9601 CCTCGGCGTC CCCCGGGACA CTGGTGCGGT ACCGGTAAGC CGTGTCGTCA AGAGTGGTAC 9661 CCATGGTTGT TTATGTTCGG ATGTGATGTG AGAACTGTAT CCTAGCAAGA TTTTAAAAGG 9721 AAGTATATGA AAGAAGAACC TCAGTGGCAA ATCCTAACCT TTTATATTTC TCTACAGGGG 9781 CGCGGCGTGG GGACAATTCA ACGCGTCTGT GAGGGGAGCG TTTCCCTGCT CGCAGGTCTG 9841 CAGCGAGGAG CCGTAATTTT TGCTTCGCGC CGTGCGGCCA TCAAAATGTA TGGATGCAAA 9901 TGATTATACA TGGGGATGTA TGGGCTAAAT GTACGGGCGA CAGTCACATC ATGCCCCTGA 9961 GCTGCGCACG TCAAGACTGT CAAGGAGGGT ATTCTGGGCC TCCATGTCGC TGATTACCCT 10021 GTTATCCCTA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.