pLV3-CMV-GATA3-3×FLAG-CopGFP-P

pLV3-CMV-GATA3-3×FLAG-CopGFP-Puro plasmid

PVT108119

pLV3-CMV-GATA3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-GATA3(cattle)-3×FLAG-CopGFP-Puro
Alias: NM_001076804.1
Gene lenght: 1329
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Bovine
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-GATA3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-GATA3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-GATA3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg aggtgacggc ggaccagcct 2341 cgctgggtga gccatcacca ccccgcggtc ctcaacgggc agcacccgga cactcaccac 2401 ccgggcctcg gccactccta catggacccg gcgcagtacc ctctgcccga ggaggtggat 2461 gtacttttta acatcgacgg tcaaggcaac cacgtcccgt cctactacgg aaactcggtg 2521 agggccactg tgcagaggta tcctccgacc caccatggga gccaggtgtg ccggccacct 2581 ctgctgcacg gatccctgcc ctggctggat ggtggcaaag ccctgggcag ccatcacacc 2641 gcttcgccct ggaacctcag ccccttctcc aagacgtcca tccaccacgg ctccccgggg 2701 cccctgtcgg tctacccgcc ggcctcgtcc tcctccctgt cggcggggca ctccagccca 2761 cacctcttca ccttcccgcc caccccgccg aaggacgtct ccccggaccc atccctgtcc 2821 accccgggct ccgccggctc cggccgccag gacgagaagg agtgcatcaa gtaccaggtg 2881 ccgctgcccg acagcatgaa gctggagtcg gcacaccccc gcggcagcat ggccaccctg 2941 ggaggggcgg cggcctcggc ccaccacccc atcaccacct acccaccata cgtccccgag 3001 tacagctctg gactcttccc gcccagcagc ctccttgggg gctcccccac cggcttcggg 3061 tgcaagtcga ggcccaaggc gagatccagc acaggccggg agtgtgtgaa ctgtggggcg 3121 acatccaccc ctctctggcg acgagacgga accgggcatt acctgtgtaa cgcctgcggg 3181 ctctaccaca agatgaacgg acagaaccgg cccctcatca agcccaagcg aaggctgtcg 3241 gcagcaagaa gggcaggtac gtcctgtgca aactgtcaaa ccacaactac gactctgtgg 3301 agaagaaatg ccaatggaga ccctgtctgc aatgcctgtg ggctctacta taagctgcac 3361 aatattaaca gacccctgac tatgaagaag gaagggatcc agaccagaaa ccgaaaaatg 3421 tctagcaaat ccaaaaagtg caaaaaggtg cacgatgcgc tggaggactt ccccaagagc 3481 agctcgttca accccgccgc cctctccaga cacatgtctt cgcttagtca catctcgccc 3541 ttcagccact ccagccacat gctgaccacg cccacaccga tgcacccgcc gtccagcctc 3601 tccttcggac ctcaccaccc ttccagtatg gtcaccgcca tgggcggatc cGACTACAAA 3661 GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGtag 3721 cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 3781 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 3841 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 3901 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 3961 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 4021 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 4081 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 4141 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 4201 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 4261 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg 4321 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 4381 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 4441 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 4501 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 4561 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 4621 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 4681 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 4741 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 4801 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 4861 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 4921 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 4981 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 5041 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 5101 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 5161 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 5221 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 5281 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 5341 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 5401 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 5461 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 5521 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 5581 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 5641 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat 5701 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 5761 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 5821 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 5881 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 5941 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 6001 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 6061 ggcactgaca attccgtggt gttgtcgggg aaATCATCGT CCTTTCCTtg gctgctcgcc 6121 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 6181 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctTcgc 6241 cttcgccctc agacgagtcg gatctccctt tggGccgcct ccccgcctgg tacctttaag 6301 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 6361 ggaagggcta attcactccc aacgaaGata agatctgctt tttgcttgta ctgggtctct 6421 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 6481 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 6541 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 6601 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 6661 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 6721 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 6781 atcttatcat gtctggctct agctaTCCCG CCCCTAACTC CGCCCAtccc gcccctaact 6841 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 6901 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 6961 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg 7021 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 7081 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 7141 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 7201 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 7261 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7321 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7381 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7441 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7501 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7561 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7621 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7681 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7741 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7801 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7861 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7921 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7981 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 8041 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 8101 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 8161 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 8221 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 8281 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8341 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8401 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8461 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8521 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8581 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8641 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8701 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8761 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8821 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8881 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8941 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 9001 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 9061 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 9121 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 9181 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 9241 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 9301 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 9361 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 9421 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 9481 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 9541 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 9601 gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.