pCMV-USP8-3×HA-mCMV-EGFP-Neo p

pCMV-USP8-3×HA-mCMV-EGFP-Neo plasmid

PVT108062

pCMV-USP8-3×HA-mCMV-EGFP-Neo plasmid Information

Plasmid name: pCMV-USP8(human)-3×HA-mCMV-EGFP-Neo
Alias: NM_005154.5;HumORF8; PITA4; SPG59; UBPY
Gene lenght: 3354
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F(special construction)
Reverse primer:
Copy number: High
Induction:

pCMV-USP8-3×HA-mCMV-EGFP-Neo plasmid Description

pCMV-USP8-3×HA-mCMV-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-USP8-3×HA-mCMV-EGFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCGC CACCatgcct gctgtggctt cagttcctaa 661 agaactctac ctcagttctt cactaaaaga ccttaataag aagacagaag ttaaaccaga 721 gaaaataagc actaagagtt atgtgcacag tgccctgaag atctttaaga cagcagaaga 781 atgcagatta gatcgtgatg aggaaagggc ctatgtacta tatatgaaat acgtgactgt 841 ttataatctt atcaaaaaaa gacctgattt caagcaacag caggattatt tccattcaat 901 acttggacct ggaaacatca aaaaagctgt cgaagaagct gaaagactct ctgaaagcct 961 taaattaaga tatgaagaag ctgaagtccg gaaaaaactt gaggaaaaag acaggcagga 1021 ggaagcacag cggctacaac aaaaaaggca ggaaacagga agagaggatg gtggcacatt 1081 ggctaaaggc tctttggaga atgttttgga ttccaaagac aaaacccaaa agagcaatgg 1141 tgaaaagaat gaaaaatgtg agaccaaaga gaaaggagca atcacagcaa aggaactata 1201 cacaatgatg acggataaaa acatcagctt gattataatg gatgctcgaa gaatgcagga 1261 ttatcaggat tcctgtattt tacattctct cagtgttcct gaagaagcca tcagtccagg 1321 agtcactgct agttggattg aagcacacct gccagatgat tctaaagaca catggaagaa 1381 gagggggaat gtggagtatg tggtacttct tgactggttt agttctgcca aagatttaca 1441 gattggaaca actctccgga gtctgaaaga tgcacttttc aagtgggaaa gtaaaactgt 1501 cctgcgcaat gagcctttgg ttttagaggg aggctatgaa aactggctcc tttgttatcc 1561 ccagtataca acaaatgcta aggtcactcc acccccacga cgccagaatg aagaggtgtc 1621 tatctcattg gattttactt atccctcatt ggaagaatca attccttcta aacctgctgc 1681 ccagacgcca cctgcatcta tagaagtaga tgaaaatata gaattgataa gtggtcaaaa 1741 tgagagaatg ggaccactga atatatcaac tccagttgaa ccagttgctg cttctaaatc 1801 tgatgtttca cccataattc agccagtgcc tagtataaag aatgttccac agattgatcg 1861 tactaaaaaa ccagcagtca aattgcctga agagcataga ataaaatctg aaagtacaaa 1921 ccatgagcaa caatctcctc agagtggaaa agttattcct gatcgttcca ccaagccagt 1981 agttttttct ccaactctca tgttaacaga tgaagaaaag gctcgtattc atgcagaaac 2041 tgctcttcta atggaaaaaa acaaacaaga aaaagaactt cgggaaaggc agcaagagga 2101 acagaaagag aaactgagga aggaagaaca agaacaaaaa gccaaaaaga aacaagaagc 2161 tgaagaaaat gaaattacag agaagcaaca aaaagcaaaa gaagaaatgg agaagaaaga 2221 aagtgaacag gccaagaaag aagataaaga aacctcagca aagaggggca aagaaataac 2281 aggagtaaaa agacaaagta aaagtgaaca tgaaacttct gatgccaaga aatctgtaga 2341 agataggggg aaaaggtgtc caaccccaga aatacagaaa aagtcaacag gagatgtgcc 2401 ccatacatct gtgacagggg attcaggttc aggcaagcca tttaagatta aaggacaacc 2461 agaaagtgga attctaagga caggaacttt tagagaggat acagacgata ccgaaagaaa 2521 taaagctcaa cgagaacctt tgacaagagc acgaagtgaa gaaatgggga ggatcgtacc 2581 aggactgcct tcaggctggg ccaagtttct tgacccaatc actggaacct ttcgttatta 2641 tcattcaccc accaacactg ttcatatgta cccaccggaa atggctcctt catctgcacc 2701 tccttccacc cctccaactc ataaagccaa gccacagatt cctgctgagc gggataggga 2761 accttccaaa ctgaagcgct cctactcctc cccagatata acccaggcta ttcaagagga 2821 agagaagagg aagccaacag taactccaac agttaatcgg gaaaacaagc caacatgtta 2881 tcctaaagct gagatctcaa ggctttctgc ttctcagatt cggaacctca atcctgtttt 2941 tggaggttct ggaccagctc ttactggact tcgtaactta ggaaatactt gttatatgaa 3001 ctcaatattg cagtgcctat gtaacgctcc acatttggct gattatttca accgaaactg 3061 ttatcaggat gatattaaca ggtcaaattt gttggggcat aaaggtgaag tggcagaaga 3121 atttggtata atcatgaaag ccctgtggac aggacagtat agatatatca gtccaaagga 3181 ctttaaaatc accattggga agatcaatga ccagtttgca ggatacagtc agcaagattc 3241 acaagaattg cttctgttcc taatggatgg tctccatgaa gatctaaata aagctgataa 3301 tcggaagaga tataaagaag aaaataatga tcatctcgat gactttaaag ctgcagaaca 3361 tgcctggcag aaacacaagc agctcaatga gtctattatt gttgcacttt ttcagggtca 3421 attcaaatct acagtacagt gcctcacatg tcacaaaaag tctaggacat ttgaggcctt 3481 catgtatttg tctctaccac tagcatccac aagtaaatgt acattacagg attgccttag 3541 attattttcc aaagaagaaa aactcacaga taacaacaga ttttactgca gtcattgcag 3601 agctcgacgg gattctctaa aaaagataga aatctggaag ttaccacctg tgcttttagt 3661 gcatctgaaa cgtttttcct acgatggcag gtggaaacaa aaattacaga catctgtgga 3721 cttcccgtta gaaaatcttg acttgtcaca gtatgttatt ggtccaaaga acaatttgaa 3781 gaaatataat ttgttttctg tttcaaatca ctacggtggg ctggatggag gccactacac 3841 agcctattgt aaaaatgcag caagacaacg gtggtttaag tttgatgatc atgaagtttc 3901 tgatatctcc gtttcttctg tgaaatcttc agcagcttat atcctctttt atacttcatt 3961 gggaccacga gtaactgatg tagccacaTA CCCATACGAC GTACCTGATT ATGCAACTTA 4021 TCCTTATGAT GTACCTGATT ATGCTAGCTA TCCTTATGAT GTCCCAGACT ACGCATAGGG 4081 ATCCGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC 4141 TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT 4201 TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG 4261 GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG 4321 GGATGCGGTG GGCTCTATGG TCTAGAGATA TACTGAGTCA TTAGGGACTT TCCAATGGGT 4381 TTTGCCCAGT ACATAAGGTC AATAGGGGTG AATCAACAGG AAAGTCCCAT TGGAGCCAAG 4441 TACACTGAGT CAATAGGGAC TTTCCATTGG GTTTTGCCCA GTACAAAAGG TCAATAGGGG 4501 GTGAGTCAAT GGGTTTTTCC CATTATTGGC ACGTACATAA GGTCAATAGG GGTGAGTCAT 4561 TGGGTTTTTC CAGCCAaTTT AATTAAAACG CCATGTACTT TCCCACCATT GACGTCAATG 4621 GGCTATTGAA ACTAATGCAA CGTGACCTTT AAACGGTACT TTCCCATAGC TGATTAATGG 4681 GAAAGTACCG TTCTCGAGCC AATACACGTC AATGGGAAGT GAAAGGGCAG CCAAAACGTA 4741 ACACCGCCCC GGTTTTCCCC TGGAAATTCC ATATTGGCAC GCATTCTATT GGCTGAGCTG 4801 CGTTCTACGT GGGTATAAGA GGCGCGACCA GCGTCGGTAC CGTCGCAGTC TTCGGTCTGA 4861 CCACCGTAGA ACGCAGATCa ccggtcgcca ccatggtgag caagggcgag gagctgttca 4921 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 4981 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 5041 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 5101 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 5161 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 5221 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 5281 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 5341 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 5401 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 5461 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 5521 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 5581 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag 5641 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa 5701 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg 5761 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 5821 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata 5881 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 5941 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 6001 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 6061 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 6121 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 6181 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 6241 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 6301 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 6361 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 6421 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt 6481 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 6541 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 6601 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 6661 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 6721 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 6781 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc 6841 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 6901 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 6961 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 7021 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 7081 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 7141 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 7201 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 7261 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 7321 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg 7381 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 7441 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 7501 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 7561 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 7621 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa 7681 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 7741 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 7801 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg 7861 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa 7921 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg 7981 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc 8041 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat 8101 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 8161 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 8221 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 8281 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 8341 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 8401 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 8461 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 8521 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 8581 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 8641 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 8701 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 8761 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 8821 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 8881 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 8941 catgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.