pLV3-CMV-C1QTNF5-3×FLAG-CopGFP

pLV3-CMV-C1QTNF5-3×FLAG-CopGFP-Puro plasmid

PVT108043

pLV3-CMV-C1QTNF5-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-C1QTNF5(human)-3×FLAG-CopGFP-Puro
Alias: NM_015645.5;CTRP5; MFRP
Gene lenght: 729
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-C1QTNF5-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-C1QTNF5-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-C1QTNF5-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatga ggccactcct cgtcctgctg 2341 ctcctgggcc tggcggccgg ctcgccccca ctggacgaca acaagatccc cagcctctgc 2401 ccggggcacc ccggccttcc aggcacgccg ggccaccatg gcagccaggg cttgccgggc 2461 cgcgatggcc gcgacggccg cgacggcgcg cccggggctc cgggagagaa aggcgagggc 2521 gggaggccgg gactgccggg acctcgaggg gaccccgggc cgcgaggaga ggcgggaccc 2581 gcggggccca ccgggcctgc cggggagtgc tcggtgcctc cgcgatccgc cttcagcgcc 2641 aagcgctccg agagccgggt gcctccgccg tctgacgcac ccttgccctt cgaccgcgtg 2701 ctggtgaacg agcagggaca ttacgacgcc gtcaccggca agttcacctg ccaggtgcct 2761 ggggtctact acttcgccgt ccatgccacc gtctaccggg ccagcctgca gtttgatctg 2821 gtgaagaatg gcgaatccat tgcctctttc ttccagtttt tcggggggtg gcccaagcca 2881 gcctcgctct cggggggggc catggtgagg ctggagcctg aggaccaagt gtgggtgcag 2941 gtgggtgtgg gtgactacat tggcatctat gccagcatca agacagacag caccttctcc 3001 ggatttctgg tgtactccga ctggcacagc tccccagtct ttgctggatc cGACTACAAA 3061 GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGtag 3121 cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 3181 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 3241 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 3301 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 3361 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 3421 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 3481 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 3541 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 3601 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 3661 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg 3721 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 3781 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 3841 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 3901 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 3961 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 4021 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 4081 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 4141 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 4201 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 4261 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 4321 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 4381 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 4441 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 4501 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 4561 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 4621 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 4681 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 4741 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 4801 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 4861 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 4921 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 4981 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 5041 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat 5101 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 5161 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 5221 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 5281 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 5341 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 5401 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 5461 ggcactgaca attccgtggt gttgtcgggg aaATCATCGT CCTTTCCTtg gctgctcgcc 5521 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 5581 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctTcgc 5641 cttcgccctc agacgagtcg gatctccctt tggGccgcct ccccgcctgg tacctttaag 5701 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 5761 ggaagggcta attcactccc aacgaaGata agatctgctt tttgcttgta ctgggtctct 5821 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 5881 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 5941 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 6001 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 6061 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 6121 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 6181 atcttatcat gtctggctct agctaTCCCG CCCCTAACTC CGCCCAtccc gcccctaact 6241 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 6301 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 6361 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg 6421 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 6481 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 6541 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 6601 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 6661 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 6721 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 6781 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 6841 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 6901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 6961 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7021 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7081 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7141 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7201 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7261 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 7681 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 7741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 7801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 7861 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 7921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 7981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8401 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8461 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8521 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 8581 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 8641 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 8701 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 8761 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 8821 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 8881 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 8941 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 9001 gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.