pCMV-CEP97-3×FLAG-BioID2-Neo p

pCMV-CEP97-3×FLAG-BioID2-Neo plasmid

PVT107956

pCMV-CEP97-3×FLAG-BioID2-Neo plasmid Information

Plasmid name: pCMV-CEP97(human)-3×FLAG-BioID2-Neo
Alias: NM_024548.4;2810403B08Rik; LRRIQ2
Gene lenght: 2595
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-CEP97-3×FLAG-BioID2-Neo plasmid Description

pCMV-CEP97-3×FLAG-BioID2-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-CEP97-3×FLAG-BioID2-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCGCCACC atggcggtgg cgcgcgtgga 961 cgcggctttg cctcccggag aaggatcagt ggtcaattgg tcaggacagg gactacagaa 1021 attaggtcca aatttaccct gtgaagctga tattcacact ttgattctgg ataaaaatca 1081 gattattaaa ttggaaaatc tggagaaatg caaacgatta atacagttat cagtagctaa 1141 taatcggctg gttcggatga tgggtgtggc caagctgacg ttgcttcgtg tattaaattt 1201 gcctcataat agcattggct gtgtggaagg gctaaaggaa ctagtacatc tggaatggct 1261 gaatttggca ggaaataatc ttaaggccat ggaacagatc aatagctgca cagctctaca 1321 gcatctcgat ttatcagaca ataatatatc ccagataggt gatctatcta aattggtatc 1381 cctgaaaacc ctgcttttac atggaaacat catcacctct cttagaatgg cacctgctta 1441 cctacccaga agtcttgcta tactttcttt ggcagaaaat gaaatccgag acttaaatga 1501 gatctctttt ttggcatcct taactgaatt ggaacagttg tcgattatga acaatccttg 1561 tgtgatggca acaccatcca tcccaggatt tgactatcgg ccgtacatcg tcagctggtg 1621 cctaaacctc agagtcctag atggatatgt gatttctcag aaggaaagtt tgaaagctga 1681 atggctctat agtcaaggca aggggagagc atatcggcct ggccagcaca tccagcttgt 1741 ccaatatctg gctacagtct gccccctcac ttctacacta ggtcttcaaa ctgcagagga 1801 tgccaaacta gagaagattt tgagcaaaca gaggtttcac cagaggcagt tgatgaacca 1861 aagccaaaat gaagagttgt ctcctcttgt tcctgttgaa acaagggcat cccttattcc 1921 tgagcattca agccctgttc aagattgcca gatatcccag gaaagtgaac ccgtcattca 1981 agtgaattct tgggttggga taaacagtaa tgatgatcag ttatttgcgg ttaagaataa 2041 ttttccagcc tctgtacaca ctacgagata ttctcgaaat gatctgcacc tggaagacat 2101 acagacggat gaggacaagt taaactgtag tcttctctct tcagagtcta cttttatgcc 2161 agttgcatca ggactgtctc cactatcacc tacagttgag ctgaggctgc agggcattaa 2221 cttgggccta gaagatgatg gtgttgcaga tgaatctgtg aaagggctgg aaagccaggt 2281 gttggataag gaagaggaac agcctttatg ggctgcaaat gagaattctg ttcaaatgat 2341 gagaagtgaa atcaatacag aggtaaatga gaaagctgga ctattacctt gtcctgagcc 2401 aacaataatc agtgctatct tgaaggatga taaccacagt cttacatttt ttcctgagtc 2461 aactgagcag aaacaatcag acataaagaa accagaaaat acacaaccag aaaataaaga 2521 aaccatatct caagcaactt cagagaaact tcccatgatt ttaacccaga gatctgttgc 2581 tttgggacaa gacaaagttg cccttcagaa attaaatgat gcagccacca agcttcaggc 2641 ctgttggcgg ggattttatg ccaggaacta caaccctcaa gccaaagatg tgcgttacga 2701 aatccggcta cgcagaatgc aagagcacat tgtctgctta actgatgaaa taaggagatt 2761 acgaaaagaa agagatgaag aacgtattaa aaaatttgta caagaagaag ctttcagatt 2821 cctttggaac caggtaaggt ctctacaggt ttggcaacag acagtggacc agcgtctaag 2881 ttcctggcat actgatgttc ctcctatatc aagtactctt gtgccatcga aacatccatt 2941 atttacccaa agccaggagt cctcttgtga tcaaaatgct gattggttta ttgcttctga 3001 tgtagctcct caagagaaat cattaccaga atttccagac tctggttttc attcctctct 3061 aacagaacaa gttcattcat tgcagcattc tttggatttt gagaaaagtt ccacagaagg 3121 cagtgaaagc tccataatgg ggaattccat tgacacagtc agatatggca aagaatcaga 3181 tttaggggat gttagtgaag aacatggtga atggaataag gaaagctcaa ataacgagca 3241 ggacaatagt ctgcttgaac agtatttaac ttcagttcaa cagctggaag atgctgatga 3301 gaggaccaat tttgatacag agacaagaga tagcaaactt cacattgctt gtttcccagt 3361 acagttagat acattgtctg acggtgcttc tgtagatgag agtcatggca tatctcctcc 3421 tttgcaaggt gaaattagcc agacacaaga gaattctaaa ttaaatgcag aagttcaggg 3481 gcagcagcca gaatgtgatt ctacatttca gctattgcat gttggtgtta ctgtgCTCGA 3541 GGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA 3601 TGACAAGTTC AAGAACCTGA TCTGGCTGAA GGAGGTGGAC AGCACCCAGG AGAGACTGAA 3661 GGAGTGGAAC GTGAGCTACG GCACCGCCCT GGTGGCCGAC AGACAGACCA AGGGCAGAGG 3721 CGGCCTGGGC AGAAAGTGGC TGAGCCAGGA GGGCGGCCTG TACTTCAGCT TCCTGCTGAA 3781 CCCCAAGGAG TTCGAGAACC TGCTGCAGCT GCCCCTGGTG CTGGGCCTGA GCGTGAGCGA 3841 GGCCCTGGAG GAGATCACCG AGATCCCCTT CAGCCTGAAG TGGCCCAACG ACGTGTACTT 3901 CCAGGAGAAG AAGGTGAGCG GCGTGCTGTG CGAGCTGAGC AAGGACAAGC TGATCGTGGG 3961 CATCGGCATC AACGTGAACC AGAGAGAGAT CCCCGAGGAG ATCAAGGACA GAGCCACCAC 4021 CCTGTACGAG ATCACCGGCA AGGACTGGGA CAGAAAGGAG GTGCTGCTGA AGGTGCTGAA 4081 GAGAATCAGC GAGAACCTGA AGAAGTTCAA GGAGAAGAGC TTCAAGGAGT TCAAGGGCAA 4141 GATCGAGAGC AAGATGCTGT ACCTGGGCGA GGAGGTGAAG CTGCTGGGCG AGGGCAAGAT 4201 CACCGGCAAG CTGGTGGGCC TGAGCGAGAA GGGCGGCGCC CTGATCCTGA CCGAGGAGGG 4261 CATCAAGGAG ATCCTGAGCG GCGAGTTCAG CCTGAGAAGA AGCTAGTGAG GGCCCGTTTA 4321 AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC 4381 CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA 4441 GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA 4501 GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC 4561 TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG 4621 TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC 4681 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG 4741 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG 4801 GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG 4861 ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT 4921 CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 4981 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA 5041 ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 5101 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 5161 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 5221 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 5281 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG 5341 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 5401 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 5461 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 5521 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 5581 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 5641 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 5701 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 5761 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 5821 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 5881 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 5941 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 6001 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 6061 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT 6121 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 6181 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 6241 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 6301 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 6361 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 6421 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 6481 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 6541 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 6601 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 6661 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 6721 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 6781 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 6841 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 6901 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 6961 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 7021 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 7081 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 7141 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 7201 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 7261 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 7321 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 7381 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 7441 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 7501 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 7561 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 7621 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 7681 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 7741 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 7801 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 7861 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 7921 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 7981 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 8041 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 8101 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 8161 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 8221 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 8281 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 8341 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 8401 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 8461 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 8521 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 8581 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 8641 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 8701 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT C

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.