pLV3-CMV-ASPH-3×Myc-CopGFP-Neo

pLV3-CMV-ASPH-3×Myc-CopGFP-Neo plasmid

PVT107951

pLV3-CMV-ASPH-3×Myc-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-ASPH(human)-3×Myc-CopGFP-Neo
Alias: NM_004318.4;AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN;junctin
Gene lenght: 2274
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ASPH-3×Myc-CopGFP-Neo plasmid Description

pLV3-CMV-ASPH-3×Myc-CopGFP-Neo It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ASPH-3×Myc-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggccca gcgtaagaat gccaagagca gcggcaacag 2341 cagcagcagc ggctccggca gcggtagcac gagtgcgggc agcagcagcc ccggggcccg 2401 gagagagaca aagcatggag gacacaagaa tgggaggaaa ggcggactct caggaacttc 2461 attcttcacg tggtttatgg tgattgcatt gctgggcgtc tggacatctg tagctgtcgt 2521 ttggtttgat cttgttgact atgaggaagt tctaggaaaa ctaggaatct atgatgctga 2581 tggtgatgga gattttgatg tggatgatgc caaagtttta ttaggactta aagagagatc 2641 tacttcagag ccagcagtcc cgccagaaga ggctgagcca cacactgagc ccgaggagca 2701 ggttcctgtg gaggcagaac cccagaatat cgaagatgaa gcaaaagaac aaattcagtc 2761 ccttctccat gaaatggtac acgcagaaca tgttgaggga gaagacttgc aacaagaaga 2821 tggacccaca ggagaaccac aacaagagga tgatgagttt cttatggcga ctgatgtaga 2881 tgatagattt gagaccctgg aacctgaagt atctcatgaa gaaaccgagc atagttacca 2941 cgtggaagag acagtttcac aagactgtaa tcaggatatg gaagagatga tgtctgagca 3001 ggaaaatcca gattccagtg aaccagtagt agaagatgaa agattgcacc atgatacaga 3061 tgatgtaaca taccaagtct atgaggaaca agcagtatat gaacctctag aaaatgaagg 3121 gatagaaatc acagaagtaa ctgctccccc tgaggataat cctgtagaag attcacaggt 3181 aattgtagaa gaagtaagca tttttcctgt ggaagaacag caggaagtac caccagaaac 3241 aaatagaaaa acagatgatc cagaacaaaa agcaaaagtt aagaaaaaga agcctaaact 3301 tttaaataaa tttgataaga ctattaaagc tgaacttgat gctgcagaaa aactccgtaa 3361 aaggggaaaa attgaggaag cagtgaatgc atttaaagaa ctagtacgca aataccctca 3421 gagtccacga gcaagatatg ggaaggcgca gtgtgaggat gatttggctg agaagaggag 3481 aagtaatgag gtgctacgtg gagccatcga gacctaccaa gaggtggcca gcctacctga 3541 tgtccctgca gacctgctga agctgagttt gaagcgtcgc tcagacaggc aacaatttct 3601 aggtcatatg agaggttccc tgcttaccct gcagagatta gttcaactat ttcccaatga 3661 tacttcctta aaaaatgacc ttggcgtggg atacctcttg ataggagata atgacaatgc 3721 aaagaaagtt tatgaagagg tgctgagtgt gacacctaat gatggctttg ctaaagtcca 3781 ttatggcttc atcctgaagg cacagaacaa aattgctgag agcatcccat atttaaagga 3841 aggaatagaa tccggagatc ctggcactga tgatgggaga ttttatttcc acctggggga 3901 tgccatgcag agggttggga acaaagaggc atataagtgg tatgagcttg ggcacaagag 3961 aggacacttt gcatctgtct ggcaacgctc actctacaat gtgaatggac tgaaagcaca 4021 gccttggtgg accccaaaag aaacgggcta cacagagtta gtaaagtctt tagaaagaaa 4081 ctggaagtta atccgagatg aaggccttgc agtgatggat aaagccaaag gtctcttcct 4141 gcctgaggat gaaaacctga gggaaaaagg ggactggagc cagttcacgc tgtggcagca 4201 aggaagaaga aatgaaaatg cctgcaaagg agctcctaaa acctgtacct tactagaaaa 4261 gttccccgag acaacaggat gcagaagagg acagatcaaa tattccatca tgcaccccgg 4321 gactcacgtg tggccgcaca cagggcccac aaactgcagg ctccgaatgc acctgggctt 4381 ggtgattccc aaggaaggct gcaagattcg atgtgccaac gagaccaaga cctgggagga 4441 aggcaaggtg ctcatctttg atgactcctt tgagcacgag gtatggcagg atgcctcatc 4501 tttccggctg atattcatcg tggatgtgtg gcatccggaa ctgacaccac agcagagacg 4561 cagccttcca gcaattggat ccGAACAAAA ACTAATATCA GAGGAAGATT TGGAACAGAA 4621 ACTAATCAGT GAAGAAGATT TAGAGCAAAA GTTAATTTCC GAGGAGGACT TATAgcggcc 4681 gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 4741 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg 4801 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 4861 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 4921 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg 4981 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg 5041 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc 5101 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc 5161 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc 5221 ggcgcctacg ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat 5281 cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga 5341 gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac 5401 cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta 5461 ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac 5521 ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga 5581 ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt 5641 gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg 5701 cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta 5761 cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca 5821 gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct 5881 gggcatcgtg gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc 5941 tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga 6001 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctGCCACCat 6061 ggctgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 6121 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 6181 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 6241 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 6301 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 6361 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 6421 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 6481 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 6541 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 6601 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 6661 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 6721 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 6781 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 6841 cgagttcttc tgaGTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG 6901 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA 6961 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT 7021 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT 7081 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC 7141 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG 7201 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC 7261 GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG 7321 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT 7381 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC 7441 CTCCCCGCgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 7501 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata agatctgctt 7561 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 7621 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 7681 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 7741 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 7801 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 7861 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 7921 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 7981 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 8041 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 8101 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 8161 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 8221 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 8281 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 8341 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 8401 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 8461 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 8521 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 8581 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 8641 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 8701 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 8761 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 8821 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 8881 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 8941 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 9001 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 9061 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 9121 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 9181 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 9241 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 9301 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 9361 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 9421 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 9481 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 9541 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 9601 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 9661 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 9721 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 9781 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 9841 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 9901 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9961 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 10021 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 10081 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 10141 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 10201 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 10261 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 10321 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 10381 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 10441 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 10501 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 10561 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 10621 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 10681 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 10741 aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.