pBiFC-CMV-HA-THRB-VC155 plasmi

pBiFC-CMV-HA-THRB-VC155 plasmid

PVT107934

pBiFC-CMV-HA-THRB-VC155 plasmid Information

Plasmid name: pBiFC-CMV-HA-THRB(human)-VC155
Alias: NM_000461.5;C-ERBA-2; C-ERBA-BETA; ERBA2; GRTH; NR1A2
Gene lenght: 1383
Host: Mammalian cells
Use(s): Signal report,Bimolecular fluorescence
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pBiFC-CMV-HA-THRB-VC155 plasmid Description

pBiFC-CMV-HA-THRB-VC155 This is a plasmid suitable for bimolecular fluorescence in mammalian cells.

pBiFC-CMV-HA-THRB-VC155 plasmid Sequence

1 ATGTACCCAT ACGATGTTCC AGATTACGCT CTTATGGCCA TGGAGGCCCG AATTCacatg 61 actcccaaca gtatgacaga aaatggcctt acagcctggg acaaaccgaa gcactgtcca 121 gaccgagaac acgactggaa gctagtagga atgtctgaag cctgcctaca taggaagagc 181 cattcagaga ggcgcagcac gttgaaaaat gaacagtcgt cgccacatct catccagacc 241 acttggacta gctcaatatt ccatctggac catgatgatg tgaacgacca gagtgtctca 301 agtgcccaga ccttccaaac ggaggagaag aaatgtaaag ggtacatccc cagttactta 361 gacaaggacg agctctgtgt agtgtgtggt gacaaagcca ccgggtatca ctaccgctgt 421 atcacgtgtg aaggctgcaa gggtttcttt agaagaacca ttcagaaaaa tctccatcca 481 tcctattcct gtaaatatga aggaaaatgt gtcatagaca aagtcacgcg aaatcagtgc 541 caggaatgtc gctttaagaa atgcatctat gttggcatgg caacagattt ggtgctggat 601 gacagcaaga ggctggccaa gaggaagctg atagaggaga accgggagaa aagacggcgg 661 gaagagctgc agaagtccat cgggcacaag ccagagccca cagacgagga atgggagctc 721 atcaaaactg tcaccgaagc ccatgtggcg accaacgccc aaggcagcca ctggaagcaa 781 aaacggaaat tcctgccaga agacattgga caagcaccaa tagtcaatgc cccagaaggt 841 ggaaaggttg acttggaagc cttcagccat tttacaaaaa tcatcacacc agcaattacc 901 agagtggtgg attttgccaa aaagttgcct atgttttgtg agctgccatg tgaagaccag 961 atcatcctcc tcaaaggctg ctgcatggag atcatgtccc ttcgcgctgc tgtgcgctat 1021 gacccagaaa gtgagacttt aaccttgaat ggggaaatgg cagtgacacg gggccagctg 1081 aaaaatgggg gtcttggggt ggtgtcagac gccatctttg acctgggcat gtctctgtct 1141 tctttcaacc tggatgacac tgaagtagcc ctccttcagg ccgtcctgct gatgtcttca 1201 gatcgcccgg ggcttgcctg tgttgagaga atagaaaagt accaagatag tttcctgctg 1261 gcctttgaac actatatcaa ttaccgaaaa caccacgtga cacacttttg gccaaaactc 1321 ctgatgaagg tgacagatct gcggatgata ggagcctgcc atgccagccg cttcctgcac 1381 atgaaggtgg aatgccccac agaactcttc ccccctttgt tcttggaagt gttcgaggat 1441 GGTACCCGTC CGGCGTGCAA AATCCCGAAC GACCTGAAAC AGAAAGTCAT GAACCACGAC 1501 AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC 1561 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG 1621 CCCGACAACC ACTACCTGAG CTACCAGTCC AAACTGAGCA AAGACCCCAA CGAGAAGCGC 1681 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG 1741 CTGTACAAGT AAGCGGCCGC GGGGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC 1801 AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG 1861 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT 1921 TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTC GGATCCTCTA GAGTCGATCT 1981 GCAGGCATGC TAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 2041 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 2101 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 2161 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 2221 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 2281 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 2341 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 2401 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 2461 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 2521 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 2581 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 2641 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 2701 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 2761 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 2821 AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA 2881 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 2941 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 3001 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 3061 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 3121 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 3181 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 3241 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 3301 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 3361 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 3421 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 3481 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 3541 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 3601 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 3661 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 3721 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 3781 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 3841 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 3901 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 3961 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 4021 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 4081 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 4141 TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA 4201 AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC 4261 TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA 4321 GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG 4381 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT 4441 GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG 4501 AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG 4561 CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG 4621 CCAGTGAGTT CGAGCTTGCA TGCCTGCAGG TCGTTACATA ACTTACGGTA AATGGCCCGC 4681 CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG 4741 TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC 4801 ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG 4861 GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC 4921 AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA 4981 ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA 5041 ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG 5101 CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC 5161 GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC CTCCATAGAA 5221 GACACCGGGA CCGATCCAGC CTCCGGACTC TAGAGGATCC GGTACTAGAG GAACTGAAAA 5281 ACCAGAAAGT TAACTGGTAA GTTTAGTCTT TTTGTCTTTT ATTTCAGGTC CCGGATCCGG 5341 TGGTGGTGCA AATCAAAGAA CTGCTCCTCA GTGGATGTTG CCTTTACTTC TAGGCCTGTA 5401 CGGAAGTGTT ACTTCTGCTC TAAAAGCTGC GGAATTGTAC CCGCGGGCCC ACC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.