pLV3-CMV-HDAC1-3×FLAG-EF1a-Cop

pLV3-CMV-HDAC1-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT107845

pLV3-CMV-HDAC1-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-HDAC1(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_004964.3;GON-10; HD1; KDAC1; RPD3; RPD3L1
Gene lenght: 1446
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HDAC1-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-HDAC1-3×FLAG-EF1a-CopGFP-Neo It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-HDAC1-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggcgca gacgcagggc acccggagga aagtctgtta 2341 ctactacgac ggggatgttg gaaattacta ttatggacaa ggccacccaa tgaagcctca 2401 ccgaatccgc atgactcata atttgctgct caactatggt ctctaccgaa aaatggaaat 2461 ctatcgccct cacaaagcca atgctgagga gatgaccaag taccacagcg atgactacat 2521 taaattcttg cgctccatcc gtccagataa catgtcggag tacagcaagc agatgcagag 2581 attcaacgtt ggtgaggact gtccagtatt cgatggcctg tttgagttct gtcagttgtc 2641 tactggtggt tctgtggcaa gtgctgtgaa acttaataag cagcagacgg acatcgctgt 2701 gaattgggct gggggcctgc accatgcaaa gaagtccgag gcatctggct tctgttacgt 2761 caatgatatc gtcttggcca tcctggaact gctaaagtat caccagaggg tgctgtacat 2821 tgacattgat attcaccatg gtgacggcgt ggaagaggcc ttctacacca cggaccgggt 2881 catgactgtg tcctttcata agtatggaga gtacttccca ggaactgggg acctacggga 2941 tatcggggct ggcaaaggca agtattatgc tgttaactac ccgctccgag acgggattga 3001 tgacgagtcc tatgaggcca ttttcaagcc ggtcatgtcc aaagtaatgg agatgttcca 3061 gcctagtgcg gtggtcttac agtgtggctc agactcccta tctggggatc ggttaggttg 3121 cttcaatcta actatcaaag gacacgccaa gtgtgtggaa tttgtcaaga gctttaacct 3181 gcctatgctg atgctgggag gcggtggtta caccattcgt aacgttgccc ggtgctggac 3241 atatgagaca gctgtggccc tggatacgga gatccctaat gagcttccat acaatgacta 3301 ctttgaatac tttggaccag atttcaagct ccacatcagt ccttccaata tgactaacca 3361 gaacacgaat gagtacctgg agaagatcaa acagcgactg tttgagaacc ttagaatgct 3421 gccgcacgca cctggggtcc aaatgcaggc gattcctgag gacgccatcc ctgaggagag 3481 tggcgatgag gacgaagacg accctgacaa gcgcatctcg atctgctcct ctgacaaacg 3541 aattgcctgt gaggaagagt tctccgattc tgaagaggag ggagaggggg gccgcaagaa 3601 ctcttccaac ttcaaaaaag ccaagagagt caaaacagag gatgaaaaag agaaagaccc 3661 agaggagaag aaagaagtca ccgaagagga gaaaaccaag gaggagaagc cagaagccaa 3721 aggggtcaag gaggaggtca agttggccgg atccGACTAC AAAGACCATG ACGGTGATTA 3781 TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TAgcggccgc Gaaggatctg 3841 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt 3901 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga 3961 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag 4021 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa 4081 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc 4141 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc 4201 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag 4261 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt 4321 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct 4381 agacgccacc atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat 4441 caccggcacc ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa 4501 gcagggccgc atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta 4561 cctgctgagc cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta 4621 cgagaacccc ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa 4681 gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt 4741 gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga 4801 caagatcatc cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct 4861 ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt 4921 ggacagccac atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc 4981 catgttcgcc ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga 5041 gtaccagcac gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa 5101 ttctgccgtg gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag 5161 tcttctaaca tgcggtgacg tggaggagaa tcccggccct GCCACCatgg ctgaacaaga 5221 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 5281 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 5341 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 5401 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 5461 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 5521 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 5581 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 5641 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 5701 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 5761 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 5821 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 5881 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 5941 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 6001 aGTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA 6061 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC 6121 TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA 6181 GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC 6241 CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC 6301 CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC 6361 TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG 6421 GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC 6481 GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC 6541 GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCggta 6601 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 6661 gggggactgg aagggctaat tcactcccaa cgaaGataag atctgctttt tgcttgtact 6721 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 6781 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 6841 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 6901 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 6961 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 7021 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 7081 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg 7141 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 7201 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca 7261 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 7321 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 7381 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 7441 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 7501 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 7561 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 7621 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 7681 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 7741 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 7801 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 7861 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 7921 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 7981 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 8041 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 8101 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 8161 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 8221 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 8281 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 8341 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 8401 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 8461 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 8521 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 8581 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 8641 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 8701 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 8761 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 8821 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 8881 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 8941 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 9001 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 9061 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 9121 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 9181 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 9241 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 9301 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 9361 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 9421 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 9481 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 9541 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 9601 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 9661 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 9721 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag 9781 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 9841 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 9901 gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.