pCMV-Thbs1-3×FLAG-Neo plasmid

pCMV-Thbs1-3×FLAG-Neo plasmid

PVT107813

pCMV-Thbs1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Thbs1(mouse)-3×FLAG-Neo
Alias: NM_001313914.1;tbsp1; Thbs-1; TSP-1; TSP1
Gene lenght: 3510
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Thbs1-3×FLAG-Neo plasmid Description

pCMV-Thbs1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Thbs1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgga gctcctgcgg ggactaggtg 781 tcctgttcct gttgcatatg tgtggaagca accgcattcc agagtctggg ggagataacg 841 gtgtgtttga catctttgaa ctcattggag gtgcacgaag gggccccggt cgccgactgg 901 tgaagggcca agatctatcc agccccgcct tccgcattga gaatgccaac ctgatccccg 961 ctgtgccgga tgacaagttc caagacctac tggacgctgt gtgggccgac aaaggcttca 1021 tcttcctggc ttccttgagg cagatgaaga agacccgggg cacactcctg gctgtggaac 1081 ggaaagacaa cactggccag atcttcagtg tggtctccaa cggcaaagct ggcaccctgg 1141 acctgagcct gagcctgcca gggaagcaac aagtggtgtc agtggaggaa gctctcctgg 1201 ccactggcca gtggaagagc atcacgctgt ttgttcaaga ggaccgggct caactctaca 1261 tagactgtga taagatggag agcgcggagc tggatgtacc catccagagc atcttcacca 1321 gggatctggc cagcgttgcc aggctccgag ttgcaaaggg agatgtcaat gacaattttc 1381 agggggtgct gcagaatgtg aggtttgtct ttggaaccac cccagaagac attctcagga 1441 acaaaggctg ctccagctct accaacgtcc ttcttaccct tgacaacaac gtggtgaacg 1501 gttccagccc tgctatccgc accaactaca tcggccacaa aacaaaggac ctccaagcta 1561 tctgtggcct ctcctgtgat gaactatcca gcatggtcct ggaactgaag ggcctgcgca 1621 ccatcgtgac cactctgcag gacagcatcc gaaaagtgac ggaagagaac agagagctgg 1681 tcagtgagct gaagcggcct cccctctgct ttcacaatgg agtccagtac aagaacaacg 1741 aggagtggac tgtagacagt tgcacagagt gtcactgcca gaactcggtt accatctgca 1801 aaaaggtgtc ctgtcccatc atgccctgct ccaacgccac agttcctgat ggtgaatgct 1861 gcccacggtg ctggcccagc gactctgctg acgatggctg gtctccctgg tctgagtgga 1921 cctcctgctc tgccacatgt ggcaatggaa ttcagcaacg tggtcgttcc tgtgacagcc 1981 tcaacaacag atgcgagggc tcttcggtac agacaaggac ctgccacatt caggagtgtg 2041 acaaaagatt taaacaggat ggtggctgga gtcactggtc tccatggtcg tcctgttctg 2101 tgacctgtgg tgacggtgtg atcacaagga tccggctctg caactccccc agcccccaga 2161 tgaacgggaa gccctgtgaa ggtgaagccc gggagaccaa agcctgcaag aaagacgcct 2221 gcccaattaa tggaggctgg ggtccctggt caccatggga catctgctct gtcacctgtg 2281 gaggaggagt gcagagacgc agccgactct gtaacaaccc cacaccccag tttggaggca 2341 aagactgtgt tggcgatgtg acagaaaatc aagtttgcaa caagcaggac tgcccaattg 2401 atggatgcct gtccaatccc tgctttgctg gtgccaagtg tactagctac cctgatggta 2461 gctggaaatg tggtgcgtgt cctcctggct acagtggaaa tggcatccag tgcaaagacg 2521 tcgatgagtg caaagaagtg cctgatgctt gcttcaatca caacggagaa catcggtgca 2581 agaacacaga tcctggctac aactgcctgc cctgcccacc acgattcact ggctcacagc 2641 ccttcggccg aggtgtcgaa catgccatgg ccaacaaaca ggtgtgcaaa ccgcgaaacc 2701 cctgcacgga cgggacgcat gactgcaaca agaacgctaa gtgcaactac ctgggtcact 2761 acagtgaccc catgtaccgc tgtgagtgca agcccggcta tgcaggcaat ggcatcatct 2821 gcggagagga cacagacctg gacggctggc ctaatgaaaa cctggtgtgt gtggccaacg 2881 caacctacca ctgcaaaaag gacaactgcc ccaaccttcc caactcgggg caggaagact 2941 atgacaagga cgggattggc gatgcctgcg atgatgacga tgacaacgac aagatccccg 3001 atgacaggga caactgtcca ttccattaca acccagccca gtatgactat gacagagatg 3061 atgtgggaga ccgctgtgac aactgcccct acaaccacaa ccctgaccaa gcagacacag 3121 acaaaaacgg ggagggcgat gcctgtgctg tggacatcga tggagatgga atcctcaatg 3181 aacgagacaa ctgccagtac gtttacaacg tggaccagag ggacacggac atggatgggg 3241 ttggagatca gtgtgacaac tgccccctgg aacacaatcc agaccagctg gactctgact 3301 cagacctcat aggggacact tgtgacaaca atcaggacat cgatgaggat ggccatcaga 3361 acaatctgga caactgtccc tatgtgccta atgccaacca ggccgaccat gataaagatg 3421 gcaaaggaga tgcctgtgac catgacgatg acaatgacgg catccctgat gacagagaca 3481 actgcaggct ggtgcccaat cctgaccaga aggactctga tggtgatggc cgaggtgacg 3541 cctgcaaaga cgactttgac catgacaatg tgccagatat tgatgacatc tgtcctgaga 3601 attttgacat cagtgaaacc gatttccgac aattccagat gattcctcta gatcccaaag 3661 gaacctccca aaatgaccct aactgggttg tccgccatca gggcaaagaa cttgtccaga 3721 ctgtaaactg tgaccctgga cttgctgtag gttatgatga gtttaatgct gtggacttca 3781 gcggtacctt cttcatcaac accgagagag atgatgacta cgctggcttt gttttcggct 3841 accagtccag cagccgcttc tacgttgtga tgtggaaaca agtcacccag tcctactggg 3901 acaccaaccc cacaagggct cagggatact caggcctgtc tgtaaaggtt gtgaactcca 3961 ccaccggccc tggcgagcac ctgcggaatg cactgtggca cacaggaaac acccctggcc 4021 aggtgcgcac cctgtggcat gaccctcgcc acatcggctg gaaagatttc actgcataca 4081 gatggcgtct cagccacagg ccaaagaccg gttatatcag agtggtgatg tatgaaggaa 4141 agaaaatcat ggctgactcg ggacccatct atgacaaaac ctacgccggc ggtagactag 4201 gcctgttcgt cttctctcag gaaatggtgt tcttctcaga catgaaatac gagtgtcgag 4261 attccGGATC CGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA 4321 AGGATGACGA TGACAAGTAG TGAGGGCCCG ATATCTCGAC AATCAACCTC TGGATTACAA 4381 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA 4441 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC 4501 CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG 4561 TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC 4621 CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT 4681 CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT 4741 GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT 4801 TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC 4861 CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG 4921 TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGAAACGGG GGAGGCTAAC TGAAACACGG 4981 AAGGAGACAA TACCGGAAGG AACCCGCGCT ATGACGGCAA TAAAAAGACA GAATAAAACG 5041 CACGGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT 5101 CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC 5161 CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC 5221 TGCCATAGCA GATCTGCGCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG 5281 CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC 5341 CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC 5401 CCGTCAAGCT CTAAATCGGG GCATCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT 5461 CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC 5521 GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC 5581 TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGGGGAT 5641 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG 5701 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 5761 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 5821 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 5881 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 5941 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG 6001 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 6061 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 6121 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 6181 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 6241 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 6301 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 6361 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 6421 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 6481 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 6541 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 6601 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 6661 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 6721 ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 6781 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 6841 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 6901 GGACTCTGGG GTTCGCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT 6961 CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG 7021 CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT 7081 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC 7141 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT 7201 CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT 7261 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA 7321 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC 7381 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG 7441 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT 7501 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 7561 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 7621 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 7681 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 7741 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 7801 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT 7861 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 7921 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 7981 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 8041 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT 8101 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 8161 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 8221 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 8281 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 8341 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA 8401 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC 8461 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG 8521 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT 8581 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT 8641 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG 8701 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC 8761 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA 8821 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC 8881 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT 8941 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG 9001 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT 9061 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG 9121 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC 9181 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC 9241 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA 9301 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG 9361 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCTCCC 9421 GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT 9481 CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA 9541 ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.