pLV3-U6-ASPM-shRNA3-CopGFP-Pur

pLV3-U6-ASPM-shRNA3-CopGFP-Puro plasmid

PVT107750

pLV3-U6-ASPM-shRNA3-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-ASPM(human)-shRNA3-CopGFP-Puro
Alias: GGAGAGAATAGTAAACTTA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene interference
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F(Hairpin structure)
Reverse primer:
Copy number: High
Induction:

pLV3-U6-ASPM-shRNA3-CopGFP-Puro plasmid Description

pLV3-U6-ASPM-shRNA3-CopGFP-Puro This plasmid is suitable for gene interference in mammalian cells and can also be used for lentivirus packaging.

pLV3-U6-ASPM-shRNA3-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acttgaaagc gaaagggaaa ccagagGAGc tctctcgacg caggactcgg 481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt 541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg 601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa 661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg 721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca 781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa 841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa 901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag 961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt 1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg 1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac 1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct 1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct 1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg 1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa 1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa 1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa 1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac 1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag 1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc 1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga 1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtATCG 1801 GTtaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga 1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat 1921 tttatcgata ctagtGAGGG CCTATTTCCC ATGATTCCTT CATATTTGCA TATACGATAC 1981 AAGGCTGTTA GAGAGATAAT TAGAATTAAT TTGACTGTAA ACACAAAGAT ATTAGTACAA 2041 AATACGTGAC GTAGAAAGTA ATAATTTCTT GGGTAGTTTG CAGTTTTAAA ATTATGTTTT 2101 AAAATGGACT ATCATATGCT TACCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 2161 ATCTTGTGGA AAGGACTCAT Cgctagcgaa ttcgGGAGAG AATAGTAAAC TTActcgagT 2221 AAGTTTACTA TTCTCTCCtt ttttggatcc gcggccgcGa aggatctgcg atcgctccgg 2281 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 2341 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 2401 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 2461 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc 2521 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag 2581 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag 2641 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact 2701 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt 2761 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat 2821 ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct 2881 gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat 2941 gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca 3001 cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt 3061 cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg 3121 cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt 3181 caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg 3241 cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt 3301 cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat 3361 gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt 3421 ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc 3481 cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga 3541 cggcaccgcc ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg 3601 cggtgacgtg gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc 3661 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 3721 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga 3781 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 3841 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 3901 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 3961 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 4021 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 4081 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 4141 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 4201 gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag 4261 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 4321 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 4381 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 4441 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 4501 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 4561 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 4621 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 4681 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 4741 gccggctctg cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct 4801 ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc 4861 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat 4921 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 4981 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 5041 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 5101 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 5161 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg 5221 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 5281 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc 5341 gcccctaact ccgCCCATCC CGCCCCTAAC TCCGcccagt tccgcccatt ctccgcccca 5401 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 5461 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa 5521 tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 5581 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 5641 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 5701 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 5761 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 5821 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 5881 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 5941 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 6001 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 6061 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 6121 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 6181 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 6241 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 6301 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 6361 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 6421 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 6481 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 6541 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 6601 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 6661 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 6721 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 6781 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6841 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6901 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 6961 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 7021 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 7081 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 7141 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 7201 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 7261 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 7321 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 7381 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 7441 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 7501 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 7561 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 7621 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 7681 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 7741 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 7801 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 7861 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 7921 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 7981 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 8041 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 8101 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.