pEF1a-SMO-3×FLAG plasmid

pEF1a-SMO-3×FLAG plasmid

PVT107696

pEF1a-SMO-3×FLAG plasmid Information

Plasmid name: pEF1a-SMO(human)-3×FLAG
Alias: NM_005631.5;CRJS; FZD11; Gx; PHLS; SMOH
Gene lenght: 2361
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pEF1a-SMO-3×FLAG plasmid Description

pEF1a-SMO-3×FLAG is a plasmid suitable for protein expression in mammalian cells.

pEF1a-SMO-3×FLAG plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTg gctccggtgc 61 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg 121 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta 181 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt 241 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc 301 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg 361 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag 421 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg 481 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc 541 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct 601 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga 661 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc 721 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc 781 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc 841 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc 901 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt 961 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag 1021 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca 1081 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga 1141 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag 1201 tttttttctt ccatttcagg tgtcgtgagg aattagcttg gtaCTGGCTA GCGTTTAAAC 1261 TTAAGCTTGG TACCGAGCTC GGATCCgcca ccatggccgc tgcccgccca gcgcgggggc 1321 cggagctccc gctcctgggg ctgctgctgc tgctgctgct gggggacccg ggccgggggg 1381 cggcctcgag cgggaacgcg accgggcctg ggcctcggag cgcgggcggg agcgcgagga 1441 ggagcgcggc ggtgactggc cctccgccgc cgctgagcca ctgcggccgg gctgccccct 1501 gcgagccgct gcgctacaac gtgtgcctgg gctcggtgct gccctacggg gccacctcca 1561 cactgctggc cggagactcg gactcccagg aggaagcgca cggcaagctc gtgctctggt 1621 cgggcctccg gaatgccccc cgctgctggg cagtgatcca gcccctgctg tgtgccgtat 1681 acatgcccaa gtgtgagaat gaccgggtgg agctgcccag ccgtaccctc tgccaggcca 1741 cccgaggccc ctgtgccatc gtggagaggg agcggggctg gcctgacttc ctgcgctgca 1801 ctcctgaccg cttccctgaa ggctgcacga atgaggtgca gaacatcaag ttcaacagtt 1861 caggccagtg cgaagtgccc ttggttcgga cagacaaccc caagagctgg tacgaggacg 1921 tggagggctg cggcatccag tgccagaacc cgctcttcac agaggctgag caccaggaca 1981 tgcacagcta catcgcggcc ttcggggccg tcacgggcct ctgcacgctc ttcaccctgg 2041 ccacattcgt ggctgactgg cggaactcga atcgctaccc tgctgttatt ctcttctacg 2101 tcaatgcgtg cttctttgtg ggcagcattg gctggctggc ccagttcatg gatggtgccc 2161 gccgagagat cgtctgccgt gcagatggca ccatgaggct tggggagccc acctccaatg 2221 agactctgtc ctgcgtcatc atctttgtca tcgtgtacta cgccctgatg gctggtgtgg 2281 tttggtttgt ggtcctcacc tatgcctggc acacttcctt caaagccctg ggcaccacct 2341 accagcctct ctcgggcaag acctcctact tccacctgct cacctggtca ctcccctttg 2401 tcctcactgt ggcaatcctt gctgtggcgc aggtggatgg ggactctgtg agtgggattt 2461 gttttgtggg ctacaagaac taccgatacc gtgcgggctt cgtgctggcc ccaatcggcc 2521 tggtgctcat cgtgggaggc tacttcctca tccgaggagt catgactctg ttctccatca 2581 agagcaacca ccccgggctg ctgagtgaga aggctgccag caagatcaac gagaccatgc 2641 tgcgcctggg catttttggc ttcctggcct ttggctttgt gctcattacc ttcagctgcc 2701 acttctacga cttcttcaac caggctgagt gggagcgcag cttccgggac tatgtgctat 2761 gtcaggccaa tgtgaccatc gggctgccca ccaagcagcc catccctgac tgtgagatca 2821 agaatcgccc gagccttctg gtggagaaga tcaacctgtt tgccatgttt ggaactggca 2881 tcgccatgag cacctgggtc tggaccaagg ccacgctgct catctggagg cgtacctggt 2941 gcaggttgac tgggcagagt gacgatgagc caaagcggat caagaagagc aagatgattg 3001 ccaaggcctt ctctaagcgg cacgagctcc tgcagaaccc aggccaggag ctgtccttca 3061 gcatgcacac tgtgtcccac gacgggcccg tggcgggctt ggcctttgac ctcaatgagc 3121 cctcagctga tgtctcctct gcctgggccc agcatgtcac caagatggtg gctcggagag 3181 gagccatact gccccaggat atttctgtca cccctgtggc aactccagtg cccccagagg 3241 aacaagccaa cctgtggctg gttgaggcag agatctcccc agagctgcag aagcgcctgg 3301 gccggaagaa gaagaggagg aagaggaaga aggaggtgtg cccgctggcg ccgccccctg 3361 agcttcaccc ccctgcccct gcccccagta ccattcctcg actgcctcag ctgccccggc 3421 agaaatgcct ggtggctgca ggtgcctggg gagctgggga ctcttgccga cagggagcgt 3481 ggaccctggt ctccaaccca ttctgcccag agcccagtcc ccctcaggat ccatttctgc 3541 ccagtgcacc ggcccccgtg gcatgggctc atggccgccg acagggcctg gggcctattc 3601 actcccgcac caacctgatg gacacagaac tcatggatgc agactcggac ttcGCGGCCG 3661 CTGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG 3721 ATGACAAGTA GTGAGGGCCC GATATCTCGA CAATCAACCT CTGGATTACA AAATTTGTGA 3781 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT 3841 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA 3901 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT 3961 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT 4021 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG 4081 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC 4141 GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG 4201 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT 4261 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC 4321 CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG GAAGGAGACA 4381 ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGGTGT 4441 TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC 4501 ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC 4561 AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC 4621 AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG 4681 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC 4741 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC 4801 TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA 4861 AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG 4921 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC 4981 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA TTTCGGCCTA 5041 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG 5101 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 5161 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 5221 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 5281 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 5341 ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 5401 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 5461 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 5521 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 5581 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 5641 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 5701 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 5761 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 5821 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 5881 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 5941 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 6001 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 6061 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 6121 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 6181 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 6241 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 6301 GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC 6361 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT 6421 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC 6481 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 6541 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC 6601 GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 6661 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 6721 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 6781 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 6841 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 6901 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 6961 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 7021 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 7081 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 7141 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 7201 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT 7261 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 7321 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 7381 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 7441 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT 7501 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 7561 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 7621 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 7681 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 7741 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 7801 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 7861 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 7921 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 7981 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 8041 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 8101 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 8161 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 8221 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 8281 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 8341 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 8401 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 8461 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 8521 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 8581 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 8641 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 8701 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 8761 CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA 8821 TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT 8881 GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA 8941 GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.