pLV3-CMV-E2F2-3×FLAG-CopGFP-Pu

pLV3-CMV-E2F2-3×FLAG-CopGFP-Puro plasmid

PVT107679

pLV3-CMV-E2F2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-E2F2(human)-3×FLAG-CopGFP-Puro
Alias: NM_004091.4;E2F-2
Gene lenght: 1311
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-E2F2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-E2F2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-E2F2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgc tgcaagggcc ccgggccttg 2341 gcttcggccg ctgggcagac cccgaaggtg gtgcccgcga tgagccccac agagctgtgg 2401 ccatccggcc tcagcagccc ccagctctgc ccagctactg ctacctacta cacaccgctg 2461 tacccgcaga cggcgcctcc cgcagcggcg ccaggcacct gcctcgacgc cactccccac 2521 ggacccgagg gccaagttgt gcgatgcctg ccggcaggcc ggctgccggc caaaaggaag 2581 ctggatctgg aggggattgg gaggcccgtc gtccctgagt tcccaacccc caaggggaag 2641 tgcatcagag tggatggcct ccccagcccc aaaaccccca aatcccccgg ggagaagact 2701 cggtatgaca cttcgctggg gctgctcacc aagaagttca tttacctcct gagcgagtca 2761 gaggatgggg tcctggacct gaactgggcc gctgaggtgc tggacgtgca gaagcggcgc 2821 atctatgaca tcaccaacgt gctggaaggc atccagctca tccgcaagaa ggccaagaac 2881 aacatccagt gggtaggcag gggaatgttt gaagacccca ccagacctgg gaagcagcaa 2941 cagctggggc aggagctgaa ggagctgatg aacacggagc aggccttgga ccagctcatc 3001 cagagctgct ctctgagctt caagcacctg actgaggaca aggccaacaa gaggctggcc 3061 tatgtgactt accaggatat ccgtgctgtt ggcaacttta aggagcagac agtgattgcc 3121 gtcaaggccc ctccgcagac gagactggaa gtgcccgaca ggactgagga caacctgcag 3181 atatatctca agagcaccca agggcccatc gaagtctacc tgtgcccaga ggaggtgcag 3241 gagccggaca gtccttccga ggagcctctc ccctctacct ccaccctctg ccccagccct 3301 gactctgccc agcccagcag cagcaccgac cctagcatca tggagcccac agcatcctca 3361 gtgccagcac cagcgccaac cccccagcag gccccaccgc ctccatccct ggtccccttg 3421 gaggctactg acagcctgct ggagctgccg cacccactcc tgcagcagac tgaggaccag 3481 ttcctgtccc cgaccctggc gtgcagctcc cctctgatca gcttctcccc atccttggac 3541 caggacgact acctgtgggg cttggaggcg ggtgagggca tcagcgatct cttcgactcc 3601 tacgaccttg gggacctgtt gattaatgga tccGACTACA AAGACCATGA CGGTGATTAT 3661 AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGt agcggccgcG aaggatctgc 3721 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 3781 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa 3841 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 3901 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag 3961 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca 4021 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc 4081 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc 4141 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc 4201 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta 4261 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc 4321 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag 4381 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac 4441 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac 4501 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag 4561 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg 4621 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac 4681 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg 4741 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg 4801 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc 4861 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag 4921 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat 4981 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt 5041 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg 5101 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 5161 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 5221 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 5281 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 5341 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 5401 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 5461 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 5521 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 5581 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 5641 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa 5701 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 5761 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 5821 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 5881 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 5941 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 6001 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 6061 gtgttgtcgg ggaaATCATC GTCCTTTCCT tggctgctcg cctgtgttgc cacctggatt 6121 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 6181 cgcggcctgc tgccggctct gcggcctctt ccgcgtctTc gccttcgccc tcagacgagt 6241 cggatctccc tttggGccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc 6301 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 6361 ccaacgaaGa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 6421 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6481 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6541 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6601 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 6661 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 6721 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct 6781 ctagctaTCC CGCCCCTAAC TCCGCCCAtc ccgcccctaa ctccgcccag ttccgcccat 6841 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 6901 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac 6961 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 7021 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 7081 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 7141 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 7201 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 7261 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 7321 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 7381 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 7441 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 7501 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 7561 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 7621 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 7681 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 7741 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 7801 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 7861 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 7921 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 7981 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 8041 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 8101 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 8161 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 8221 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 8281 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 8341 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 8401 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 8461 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 8521 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 8581 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 8641 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 8701 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 8761 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 8821 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 8881 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 8941 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 9001 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 9061 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 9121 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 9181 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 9241 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 9301 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 9361 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 9421 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 9481 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 9541 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 9601 g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.