pLV3-CMV-Cpt1b-8×His-CopGFP-Pu

pLV3-CMV-Cpt1b-8×His-CopGFP-Puro plasmid

PVT107671

pLV3-CMV-Cpt1b-8×His-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Cpt1b(mouse)-8×His-CopGFP-Puro
Alias: NM_009948.2;Cpt1; Cpt1-m; Cpti; Cpti-m; M-cpti
Gene lenght: 2316
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Cpt1b-8×His-CopGFP-Puro plasmid Description

pLV3-CMV-Cpt1b-8×His-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Cpt1b-8×His-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg cggaagcaca ccaggcagta 2341 gctttccagt tcactgtgac cccagacggg gtcgacttcc ggcttagtcg ggaggctctg 2401 agacacatct acctgtctgg aatcaactcc tggaagaaac gccttattcg aatcaagaat 2461 ggcatcctta ggggtgtgta ccctggcagc cccaccagct ggctggttgt tgtcatggca 2521 acagttggtt ccaactactg caaggtggac atctccatgg gactggtcga ttgcatccag 2581 agatgcctcc cggaaaggta tggccacttt gggaccccac agacagaggc acttctcagc 2641 atggtcatct tctccaccgg agtctgggcg acaggcattt tcttcttccg acaaaccctg 2701 aagctgctgc tctcctacca cgggtggatg ttcgagatgc acagcaagac cagccatgcc 2761 accaagatct gggctatctg tgtccgtctc ctgtccagcc ggcggcccat gctctacagc 2821 ttccaaacgt cactgcctaa gcttcctgtc cccagcgtgc cagccacaat tcaccggtac 2881 ttggattctg tgcggccctt attggatgat gaagcatatt accgcatgga gacattggcc 2941 aaggaattcc aggacaagac tgcccccagg ctgcagaaat acctggtgct caagtcatgg 3001 tgggcaacta actatgtgag tgactggtgg gaagaatatg tctacctccg aagcaggagc 3061 cccctcatgg tgaacagcaa ctattatgcc atggattttg tgcttattaa gaacacaaat 3121 gtgcaagcag cccgtctagg caatgccgtt cacgccatga tcatgtatcg ccgcaaactg 3181 gaccgtgaag agatcaagcc ggtcatggca ctgggtatgg tgcccatgtg ctcctaccag 3241 atggagagga tgttcaacac tacacgcatc ccaggcaaag agacagactt gctacagcac 3301 ctctcagaga gcaggcacgt ggctgtctac cacaaaggtc gcttcttcaa ggtctggctc 3361 tatgagggct cgcgcctgct caagccccga gacctggaga tgcagttcca gagaatcctc 3421 gacgaccctt ccccacctca gcctggggag gagaagctgg cagccctcac cgcaggagga 3481 agggtagagt gggcagaggc acgtcagacc ttcttcagct ctggcaagaa caagatgtct 3541 ctggacgcca tcgaacgtgc tgctttcttt gtgaccctgg atgaagattc tcattgctac 3601 aaccctgacg atgagaccag tcttagcctc tacggcaaag ccttgctcca tggcaactgc 3661 tataacaggt ggtttgacaa atctttcacc cttatctcct gcaagaatgg cctgttaggc 3721 ctcaacaccg aacactcgtg ggcagacgcg cccatcattg ggcacctctg ggagtttgtc 3781 ctgggcactg atacctttca cctgggctac acggagacag gacactgtgt gggtgagcca 3841 aacaccacgt tgccaccccc tcagcggctg ccgtgggaca ttcccgagca gtgccgggaa 3901 gccatcgaga actcgtacca agtagccaag gcactggccg atgacgtgga gctctactgc 3961 ttccagttct taccctttgg caaaggtctt atcaagaagt gtaggaccag ccccgacgct 4021 tttgtgcaga ttgccctaca gctggctcat ttccgggaca aaggcaagtt ctgcctgact 4081 tacgaggcct cgatgacaag aatgttccga gaggggcgga ctgagactgt gcgttcctgt 4141 accaacgagt ccgcagcctt tgtgcaggcc atgatgaagg ggtcccataa gaaacaagac 4201 ctccaagatc tcttccggaa agcctccgaa aagcaccaaa acatgtaccg cctagccatg 4261 acaggggctg ggatcgaccg gcacctcttc tgcctttaca tcgtctccaa gtacttagga 4321 gttagctctc ctttcctggc tgaggtactt tctgaaccct ggagcctctc caccagccag 4381 atcccccagt tccagatctg catgtttgac ccaaaacagt atcccaatca tctgggtgct 4441 ggaggtggct ttggtcccgt ggcggatgat ggctacgggg tctcttacat gatcgcagga 4501 gaaaacacca tgttcttcca catctccagc aaatactcaa gttcagagac gaacgcccag 4561 cgctttggga accacatccg ccaagcactg ttggacatcg ccgaactttt caaaatttcc 4621 aagacagaca gcggatccCA CCATCACCAT CACCATCACC ATtagcggcc gcGaaggatc 4681 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 4741 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 4801 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 4861 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 4921 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 4981 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 5041 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 5101 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 5161 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 5221 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 5281 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 5341 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 5401 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 5461 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 5521 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 5581 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 5641 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 5701 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 5761 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 5821 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 5881 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 5941 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 6001 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 6061 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 6121 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 6181 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 6241 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 6301 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 6361 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 6421 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 6481 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 6541 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 6601 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac 6661 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 6721 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 6781 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 6841 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 6901 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 6961 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 7021 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg 7081 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 7141 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg 7201 agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag 7261 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 7321 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 7381 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 7441 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 7501 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 7561 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 7621 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 7681 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 7741 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 7801 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 7861 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat 7921 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 7981 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 8041 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 8101 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 8161 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 8221 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 8281 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 8341 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 8401 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 8461 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 8521 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 8581 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 8641 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 8701 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 8761 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 8821 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 8881 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 8941 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 9001 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 9061 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 9121 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 9181 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 9241 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 9301 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 9361 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 9421 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 9481 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 9541 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 9601 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 9661 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 9721 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 9781 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 9841 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 9901 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 9961 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 10021 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 10081 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 10141 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 10201 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 10261 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag 10321 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 10381 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 10441 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 10501 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.