pLV2-CMV-SENP2-3×Myc-Puro plas

pLV2-CMV-SENP2-3×Myc-Puro plasmid

PVT107666

pLV2-CMV-SENP2-3×Myc-Puro plasmid Information

Plasmid name: pLV2-CMV-SENP2(human)-3×Myc-Puro
Alias: NM_021627.3;AXAM2; SMT3IP2
Gene lenght: 1767
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-SENP2-3×Myc-Puro plasmid Description

pLV2-CMV-SENP2-3×Myc-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-SENP2-3×Myc-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCATGTACAG ATGGCTGGTT AGGATTCTCG GCACCATTTT 2881 CCGTTTCTGC GACCGGTCGG TGCCCCCTGC CCGGGCCCTC CTGAAGAGGC GGCGCTCAGA 2941 CAGCACTCTG TTTTCTACAG TGGACACTGA TGAAATACCA GCCAAAAGAC CAAGATTAGA 3001 TTGCTTTATT CACCAAGTGA AAAACAGTCT CTACAATGCT GCCAGCTTAT TTGGATTCCC 3061 ATTCCAGCTG ACCACAAAGC CCATGGTAAC TTCTGCTTGT AATGGAACAC GGAATGTGGC 3121 CCCTTCAGGA GAGGTATTTT CGAACTCTTC ATCTTGTGAA CTGACAGGTT CTGGATCCTG 3181 GAACAACATG CTGAAACTGG GTAATAAATC TCCTAATGGA ATAAGTGACT ATCCAAAGAT 3241 CAGAGTGACA GTTACCCGAG ATCAGCCACG CAGAGTCCTG CCTTCCTTTG GTTTTACTTT 3301 GAACTCAGAA GGCTGTAATA GAAGACCAGG TGGCCGTCGC CATAGCAAAG GTAATCCAGA 3361 GAGTTCTTTA ATGTGGAAAC CTCAGGAACA GGCTGTAACA GAGATGATTT CTGAAGAGAG 3421 TGGCAAGGGT CTGAGGCGTC CCCATTGTAC TGTGGAGGAG GGTGTTCAAA AAGAGGAAAG 3481 AGAGAAGTAC CGAAAGTTAT TGGAACGACT TAAAGAAAGT GGTCATGGAA ACTCTGTCTG 3541 TCCTGTAACT TCAAATTATC ACAGTTCTCA AAGAAGTCAG ATGGACACAT TAAAGACCAA 3601 AGGCTGGGGG GAAGAGCAAA ATCACGGAGT CAAAACAACT CAGTTTGTTC CAAAACAATA 3661 TAGACTTGTT GAAACAAGGG GACCTCTATG TTCATTGAGA AGTGAAAAGA GGTGTTCAAA 3721 GGGGAAAATT ACTGATACAG AGACGATGGT CGGAATCAGA TTTGAAAATG AAAGTAGGAG 3781 GGGATACCAA CTGGAGCCTG ACCTATCAGA AGAAGTGTCG GCCCGACTCC GCCTGGGCAG 3841 TGGAAGCAAT GGCTTACTCA GGAGGAAAGT GTCAATAATT GAGACAAAGG AAAAGAATTG 3901 CTCAGGCAAA GAGAGGGACA GAAGAACGGA CGATCTCCTT GAACTTACAG AGGACATGGA 3961 AAAGGAAATC AGTAATGCCC TAGGCCATGG CCCACAGGAT GAAATCCTAA GTAGTGCTTT 4021 CAAATTGCGA ATTACTCGAG GAGATATTCA GACATTAAAG AACTATCACT GGCTCAATGA 4081 TGAAGTCATT AATTTTTACA TGAATCTTCT GGTGGAAAGA AATAAAAAGC AAGGCTATCC 4141 AGCACTTCAT GTATTCAGTA CTTTCTTCTA TCCTAAATTA AAGTCTGGGG GTTACCAAGC 4201 AGTGAAACGA TGGACCAAAG GGGTAAATCT CTTTGAACAA GAAATTATTC TGGTGCCTAT 4261 TCATCGGAAG GTACATTGGA GCCTGGTGGT GATTGACCTA AGAAAAAAGT GTCTTAAATA 4321 TCTGGATTCT ATGGGACAAA AGGGCCACAG GATCTGTGAG ATTCTCCTTC AGTATTTACA 4381 GGATGAAAGT AAGACCAAAA GAAATAGTGA TCTGAATCTT TTAGAGTGGA CCCATCACAG 4441 CATGAAACCA CACGAGATTC CTCAACAGCT GAATGGGAGT GATTGTGGAA TGTTTACTTG 4501 TAAATATGCA GATTATATTT CTAGGGACAA ACCTATCACA TTTACTCAGC ACCAGATGCC 4561 TCTCTTCCGG AAGAAGATGG TGTGGGAAAT CCTTCATCAG CAGTTGCTGG AACAAAAACT 4621 AATATCAGAG GAAGATTTGG AACAGAAACT AATCAGTGAA GAAGATTTAG AGCAAAAGTT 4681 AATTTCCGAG GAGGACTTAT AGtctagata attctaccgg gtaggggagg cgcttttccc 4741 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg 4801 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg 4861 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca 4921 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat 4981 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct 5041 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag 5101 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac 5161 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac 5221 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 5281 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 5341 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 5401 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 5461 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5521 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5581 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5641 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5701 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5761 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 5821 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga 5881 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 5941 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 6001 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 6061 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 6121 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 6181 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 6241 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 6301 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 6361 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 6421 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga 6481 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa 6541 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac 6601 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt 6661 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc 6721 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg 6781 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac 6841 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta 6901 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag 6961 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca 7021 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca 7081 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 7141 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 7201 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 7261 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 7321 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga 7381 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 7441 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 7501 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 7561 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 7621 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 7681 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 7741 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 7801 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 7861 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 7921 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 7981 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 8041 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 8101 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 8161 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 8221 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 8281 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 8341 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 8401 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 8461 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 8521 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 8581 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 8641 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 8701 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 8761 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 8821 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 8881 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 8941 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 9001 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 9061 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 9121 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 9181 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 9241 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 9301 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 9361 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 9421 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 9481 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 9541 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct 9601 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 9661 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac 9721 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact 9781 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt 9841 taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta 9901 tttgttaaat atgtactaca aacttagtag t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.