pCMV-3×Myc-PIAS4-Neo plasmid

pCMV-3×Myc-PIAS4-Neo plasmid

PVT107660

pCMV-3×Myc-PIAS4-Neo plasmid Information

Plasmid name: pCMV-3×Myc-PIAS4(human)-Neo
Alias: NM_015897.4;PIAS-gamma; Piasg; PIASY; ZMIZ6;
Gene lenght: 1533
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×Myc-PIAS4-Neo plasmid Description

pCMV-3×Myc-PIAS4-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×Myc-PIAS4-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttAAACTT AAGCCACCAT GGCGGCGGAA CAAAAACTAA TATCAGAGGA AGATTTGGAA 961 CAGAAACTAA TCAGTGAAGA AGATTTAGAG CAAAAGTTAA TTTCCGAGGA GGACTTATCA 1021 GCGGAAAAGC TTatggcggc ggagctggtg gaggccaaaa acatggtgat gagttttcga 1081 gtctccgacc ttcagatgct cctgggtttc gtgggccgga gtaagagtgg actgaagcac 1141 gagctcgtca ccagggccct ccagctggtg cagtttgact gtagccctga gctgttcaag 1201 aagatcaagg agctgtacga gacccgctac gccaagaaga actcggagcc tgccccacag 1261 ccgcaccggc ccctggaccc cctgaccatg cactccacct acgaccgggc cggcgctgtg 1321 cccaggactc cgctggcagg ccccaatatt gactaccccg tgctctacgg aaagtactta 1381 aacggactgg gacggttgcc cgccaagacc ctcaagccag aagtccgcct ggtgaagctg 1441 ccgttcttta atatgctgga tgagctgctg aagcccaccg aattagtccc acagaacaac 1501 gagaagcttc aggagagccc gtgcatcttc gcattgacgc caagacaggt ggagttgatc 1561 cggaactcca gggaactgca gcccggagtt aaagccgtgc aggtcgtcct gagaatctgt 1621 tactcagaca ccagctgccc tcaggaggac cagtacccgc ccaacatcgc tgtgaaggtc 1681 aaccacagct actgctccgt cccgggctac tacccctcca ataagcccgg ggtggagccc 1741 aagaggccgt gccgccccat caacctcacc cacctcatgt acctgtcctc ggccaccaac 1801 cgcatcactg tcacctgggg gaactacggc aagagctact cggtggccct gtacctggtg 1861 cggcagctga cctcatcgga gctgctgcag aggctgaaga ccattggggt aaagcacccg 1921 gagctgtgca aggcactggt caaggagaag ctgcgccttg atcctgacag cgagatcgcc 1981 accaccggtg tgcgggtgtc cctcatctgt ccgctggtga agatgcggct ctccgtgccc 2041 tgccgggcag agacctgtgc ccacctgcag tgcttcgacg ccgtcttcta cctgcagatg 2101 aacgagaaga agcccacctg gatgtgcccc gtgtgcgaca agccagcccc ctacgaccag 2161 ctcatcatcg acgggctcct ctcgaagatc ctgagcgagt gtgaggacgc cgacgagatc 2221 gagtacctgg tggacggctc gtggtgcccg atccgcgccg aaaaggagcg cagctgcagc 2281 ccgcagggcg ccatcctcgt gctgggcccc tcggacgcca atgggctcct gcccgccccc 2341 agcgtcaacg ggagcggtgc cctgggcagc acgggtggcg gcggcccggt gggcagcatg 2401 gagaatggga agccgggcgc cgatgtggtg gacctcacgc tggacagctc atcgtcctcg 2461 gaggatgagg aggaggagga agaggaggag gaagacgagg acgaagaggg gccccggccc 2521 aagcgccgct gccccttcca gaagggcctg gtgccggcct gctgagaatt ctgcagatat 2581 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg 2641 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 2701 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 2761 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 2821 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 2881 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 2941 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 3001 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 3061 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 3121 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 3181 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 3241 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 3301 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 3361 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 3421 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 3481 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 3541 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 3601 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 3661 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 3721 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 3781 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 3841 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 3901 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 3961 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 4021 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 4081 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 4141 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 4201 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 4261 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 4321 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 4381 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 4441 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 4501 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 4561 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 4621 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 4681 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 4741 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 4801 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 4861 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 4921 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 4981 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 5041 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 5101 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 5161 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 5221 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 5281 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 5341 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 5401 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 5461 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 5521 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 5581 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 5641 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 5701 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 5761 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 5821 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 5881 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 5941 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 6001 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 6061 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 6121 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 6181 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 6241 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 6301 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 6361 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 6421 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 6481 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 6541 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 6601 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 6661 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 6721 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 6781 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 6841 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 6901 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 6961 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 7021 ccgaaaagtg ccacctgacg tc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.