pCMV-Clasp2-3×FLAG-Neo plasmid

pCMV-Clasp2-3×FLAG-Neo plasmid

PVT107651

pCMV-Clasp2-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Clasp2(mouse)-3×FLAG-Neo
Alias: NM_001081960.1;1500004F14Rik; 8030404L10Rik; CLASP2beta;mKIAA0627
Gene lenght: 3861
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Clasp2-3×FLAG-Neo plasmid Description

pCMV-Clasp2-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Clasp2-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgag gcggctgatt tgcaaacgga 781 tctgtgatta taaaagcttt gatgatgagg aatcagtgga tggaaatagg ccgtcgtcag 841 ctgcttcagc cttcaaggtt cctgcaccta aaacacctgg gaatcctgtc agcagtgcaa 901 gaaagcctgg ctcagcaggt ggccctaagg ttggaggtcc ttctaaagaa ggaggggctg 961 gagcagttga tgaagatgac tttataaaag cttttacaga tgttccttct gttcagatct 1021 attctagtcg agaacttgaa gagacgttaa ataagatcag ggaaattttg tcagatgaca 1081 aacatgactg ggaccagcgt gccaatgcgc ttaagaaaat cagatcactg cttgttgctg 1141 gagccgcaca gtatgattgc tttttccagc acctacgttt gttggatgga gcgcttaagc 1201 tgtcagctaa ggacctcaga tcccaggtgg tcagggaagc ttgcatcact gttgctcacc 1261 tttcaacagt cttgggaaac aaatttgatc acggcgctga agccatcgtc cctacgcttt 1321 ttaatcttgt ccccaatagt gccaaagtca tggcgacctc tggatgtgca gcaatcagat 1381 ttattattcg gcatacccat gtacctagac ttattccttt aataacaagc aactgcacat 1441 caaagtcagt tcctgtaagg agacgttcat ttgaattttt agatttgctg ctgcaagaat 1501 ggcagactca ttcactggaa agacatgcag ctgttttggt tgaaacgatt aagaagggca 1561 ttcatgatgc tgatgctgag gccagagtgg aggcaaggaa gacatacatg ggccttagga 1621 accactttcc tggtgaagct gaaacattgt acaactccct tgagccatca tatcagaaaa 1681 gtcttcaaac ttacttaaag agttctggaa gtgtagcttc tcttccgcag tcagacaggt 1741 cctcatccag ctcacaagaa agtctcaatc gtcctttttc ttctaaatgg tcaacagcaa 1801 atccttcaac tgtagctgga agagtatctg tgggaggcag caaagccaac ccccttccag 1861 gaagcctgca gcgttctcga agtgacattg atgtgaatgc ggcagctggt gccaaggctc 1921 atcatgctgc tgggcaggcg gtgcgaagtg ggcgcttagg tgcaggtgcc ctgaacccag 1981 gctcctatgc atcactagag gatacttctg acaagatgga tggaacagca tctgatgatg 2041 gtcgggtgag agccaagctt tctacaccgc ttgttgctgt gggaaatgcc aagaccgact 2101 ctagagggag aagccggaca aaaatggtgt ctcagtccca gcctggcagc cgatctgggt 2161 ctccaggaag agttctaacc acaacagccc tctctactgt gagctctggt gctcaacgag 2221 tcctggtcaa ttcagcttca gcacagaaga gaagcaagat cccaaggagc cagggctgca 2281 gcagagaggc cagcccatct cggctctcag tggcccggag cagccgtatt cctcggccga 2341 gtgtgagtca aggctgtagc cgggaagcca gcagagagag cagcagggac acgagtccgg 2401 tgcgctcctt ccagccgctg ggtccgggat atgggatcag ccagtccagc cggttgtcgt 2461 cctctgtcag tgccatgcga gtcctaaaca caggctccga tgtggaggag gcagtagctg 2521 atgccctgct cttaggagac atacggacta agaaaaaacc tgctcgaaga aggtatgaat 2581 catatggaat gcactcagat gatgatgcca acagcgatgc ctctagtgcg tgttcagaac 2641 gctcctatag ctctcgaaat ggtagtattc ctacctacat gagacagaca gaagacgtgg 2701 cagaagtcct caacagatgt gctagctcca attggtcaga gaggaaagaa ggcctcttgg 2761 gtctgcagaa cttgttaaaa aaccagagaa cgctaagtcg agttgaactg aaaagattat 2821 gtgaaatttt cacaagaatg tttgcagatc ctcatggcaa gagagtgttc agcatgttct 2881 tggagactct agtagatttc atacaagtcc acaaagatga tcttcaagat tggttgtttg 2941 ttctgctgac acagctgctg aaaaaaatgg gtgctgattt gcttggctct gttcaggcaa 3001 aagttcagaa agcccttgat attacaagag agtcttttcc aaatgatctt cagtttaata 3061 tcctaatgag atttacagtt gaccagaccc aaacgccaag cttgaaggta aaggtggcta 3121 tccttaagta catagaaact ctggcaaagc agatggaccc aagagatttt acaaattcca 3181 gtgaaactcg cctggcagtg tctcgggtca tcacttggac gacagagccc aaaagctctg 3241 atgttcggaa ggcagcgcag tcagtgctga tttctttatt tgaactcaat accccagagt 3301 ttacaatgtt actaggagct ttaccaaaaa ctttccagga tggtgctact aaacttcttc 3361 acaatcacct ccggaacact ggcaatggca cccagagttc catggggagt cctttgacga 3421 gaccaacacc tcggtcacca gccaactggt ccagtcctct tacttctcct accaacacgt 3481 ctcagaatac gttatctcca agtgcatttg attacgatac agagaacatg aattctgaag 3541 acatttatag ctcccttaga ggcgtcactg aggcaatcca gaatttcagc ttcagaagcc 3601 aagaagatat gagtgagcca gtgaggaggg accctaaaaa ggaggatggt gacacaatat 3661 gtagtggtcc tgggatgtca gatccaagag caggaggtga tgctgctgac ggcagccagc 3721 cagctctgga taataaagca tcgttgctcc actcaatgcc actccactcc tctccacgct 3781 cccgtgacta taacccatat aactactcag atagcatcag tcctttcaac aagtctgccc 3841 tcaaggaagc catgtttgat gatgacgccg accaatttcc tgatgatctt tccttagacc 3901 attctgacct agttgcagag ttgttgaagg agctgtctaa tcataatgaa cgtatagaag 3961 aaagaaaaat tgccctgtat gaactcatga agctaaccca ggaagaatct ttcagtgttt 4021 gggatgaaca cttcaaaaca atattattgt tattgcttga gacccttggg gataaagagc 4081 ctacaatccg ggccttggca ttaaaagttt taaaagaaat cttaaggcat caaccagcaa 4141 gattcaaaaa ctatgcagaa ctaactgtca tgaaaacact ggaagcacat aaagatcctc 4201 acaaagaggt ggtgagatct gctgaggaag ctgcctccgt attggctact tccattagtc 4261 cagagcagtg catcaaagtg ctttgtccca tcatacaaac cgctgactac cctattaatc 4321 tggctgcaat caaaatgcaa acaaaagtga tagagagagt atccaaggag acccttaaca 4381 tgctcttacc agagatcatg ccgggtctaa tacagggtta tgataattca gaaagcagtg 4441 tccggaaagc ttgtgtcttc tgcctggtgg ctgtccatgc agtgattggt gatgaactaa 4501 agccacatct cagtcaactc actggtagta aaatgaagct gctgaacctt tacatcaagc 4561 gtgcacagac gggctctgca ggcgcggacc ccactgctga tgtttctgga cagagtGGAT 4621 CCGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG 4681 ATGACAAGTA GTGAGGGCCC GATATCTCGA CAATCAACCT CTGGATTACA AAATTTGTGA 4741 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT 4801 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA 4861 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT 4921 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT 4981 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG 5041 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC 5101 GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG 5161 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT 5221 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC 5281 CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG GAAGGAGACA 5341 ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGGTGT 5401 TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC 5461 ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC 5521 AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC 5581 AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG 5641 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC 5701 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC 5761 TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA 5821 AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG 5881 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC 5941 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA TTTCGGCCTA 6001 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG 6061 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 6121 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 6181 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 6241 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 6301 ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 6361 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 6421 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 6481 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 6541 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 6601 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 6661 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 6721 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 6781 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 6841 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 6901 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 6961 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 7021 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 7081 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 7141 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 7201 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 7261 GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC 7321 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT 7381 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC 7441 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 7501 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC 7561 GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 7621 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 7681 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 7741 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 7801 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 7861 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 7921 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 7981 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 8041 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 8101 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 8161 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT 8221 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 8281 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 8341 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 8401 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT 8461 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 8521 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 8581 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 8641 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 8701 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 8761 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 8821 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 8881 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 8941 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 9001 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 9061 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 9121 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 9181 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 9241 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 9301 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 9361 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 9421 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 9481 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 9541 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 9601 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 9661 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 9721 CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA 9781 TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT 9841 GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA 9901 GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.