pLV3-CMV-ENG-3×FLAG-CopGFP-Pur

pLV3-CMV-ENG-3×FLAG-CopGFP-Puro plasmid

PVT107643

pLV3-CMV-ENG-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ENG(human)-3×FLAG-CopGFP-Puro
Alias: NM_001114753.3;END; HHT1; ORW1
Gene lenght: 1974
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ENG-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ENG-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ENG-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg accgcggcac gctccctctg 2341 gctgttgccc tgctgctggc cagctgcagc ctcagcccca caagtcttgc agaaacagtc 2401 cattgtgacc ttcagcctgt gggccccgag aggggcgagg tgacatatac cactagccag 2461 gtctcgaagg gctgcgtggc tcaggccccc aatgccatcc ttgaagtcca tgtcctcttc 2521 ctggagttcc caacgggccc gtcacagctg gagctgactc tccaggcatc caagcaaaat 2581 ggcacctggc cccgagaggt gcttctggtc ctcagtgtaa acagcagtgt cttcctgcat 2641 ctccaggccc tgggaatccc actgcacttg gcctacaatt ccagcctggt caccttccaa 2701 gagcccccgg gggtcaacac cacagagctg ccatccttcc ccaagaccca gatccttgag 2761 tgggcagctg agaggggccc catcacctct gctgctgagc tgaatgaccc ccagagcatc 2821 ctcctccgac tgggccaagc ccaggggtca ctgtccttct gcatgctgga agccagccag 2881 gacatgggcc gcacgctcga gtggcggccg cgtactccag ccttggtccg gggctgccac 2941 ttggaaggcg tggccggcca caaggaggcg cacatcctga gggtcctgcc gggccactcg 3001 gccgggcccc ggacggtgac ggtgaaggtg gaactgagct gcgcacccgg ggatctcgat 3061 gccgtcctca tcctgcaggg tcccccctac gtgtcctggc tcatcgacgc caaccacaac 3121 atgcagatct ggaccactgg agaatactcc ttcaagatct ttccagagaa aaacattcgt 3181 ggcttcaagc tcccagacac acctcaaggc ctcctggggg aggcccggat gctcaatgcc 3241 agcattgtgg catccttcgt ggagctaccg ctggccagca ttgtctcact tcatgcctcc 3301 agctgcggtg gtaggctgca gacctcaccc gcaccgatcc agaccactcc tcccaaggac 3361 acttgtagcc cggagctgct catgtccttg atccagacaa agtgtgccga cgacgccatg 3421 accctggtac taaagaaaga gcttgttgcg catttgaagt gcaccatcac gggcctgacc 3481 ttctgggacc ccagctgtga ggcagaggac aggggtgaca agtttgtctt gcgcagtgct 3541 tactccagct gtggcatgca ggtgtcagca agtatgatca gcaatgaggc ggtggtcaat 3601 atcctgtcga gctcatcacc acagcggaaa aaggtgcact gcctcaacat ggacagcctc 3661 tctttccagc tgggcctcta cctcagccca cacttcctcc aggcctccaa caccatcgag 3721 ccggggcagc agagctttgt gcaggtcaga gtgtccccat ccgtctccga gttcctgctc 3781 cagttagaca gctgccacct ggacttgggg cctgagggag gcaccgtgga actcatccag 3841 ggccgggcgg ccaagggcaa ctgtgtgagc ctgctgtccc caagccccga gggtgacccg 3901 cgcttcagct tcctcctcca cttctacaca gtacccatac ccaaaaccgg caccctcagc 3961 tgcacggtag ccctgcgtcc caagaccggg tctcaagacc aggaagtcca taggactgtc 4021 ttcatgcgct tgaacatcat cagccctgac ctgtctggtt gcacaagcaa aggcctcgtc 4081 ctgcccgccg tgctgggcat cacctttggt gccttcctca tcggggccct gctcactgct 4141 gcactctggt acatctactc gcacacgcgt tcccccagca agcgggagcc cgtggtggcg 4201 gtggctgccc cggcctcctc ggagagcagc agcaccaacc acagcatcgg gagcacccag 4261 agcaccccct gctccaccag cagcatggca ggatccGACT ACAAAGACCA TGACGGTGAT 4321 TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGtagcggcc gcGaaggatc 4381 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 4441 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 4501 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 4561 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 4621 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 4681 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 4741 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 4801 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 4861 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 4921 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 4981 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 5041 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 5101 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 5161 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 5221 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 5281 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 5341 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 5401 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 5461 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 5521 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 5581 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 5641 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 5701 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 5761 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 5821 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 5881 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 5941 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 6001 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 6061 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 6121 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 6181 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 6241 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 6301 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac 6361 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 6421 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 6481 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 6541 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 6601 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 6661 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 6721 gtggtgttgt cggggaaATC ATCGTCCTTT CCTtggctgc tcgcctgtgt tgccacctgg 6781 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 6841 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tTcgccttcg ccctcagacg 6901 agtcggatct ccctttggGc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 6961 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 7021 ctcccaacga aGataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 7081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 7141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 7201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 7261 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 7321 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 7381 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 7441 gctctagcta TCCCGCCCCT AACTCCGCCC Atcccgcccc taactccgcc cagttccgcc 7501 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 7561 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 7621 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat 7681 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 7741 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 7801 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 7861 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 7921 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 7981 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 8041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 8101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 8161 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 8221 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 8281 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 8341 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 8401 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 8461 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 8521 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 8581 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 8641 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 8701 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 8761 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 8821 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 8881 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 8941 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 9001 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 9061 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 9121 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 9181 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 9241 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 9301 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 9361 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 9421 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 9481 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 9541 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 9601 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 9661 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 9721 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 9781 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 9841 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 9901 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 9961 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 10021 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 10081 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 10141 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 10201 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 10261 gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.