pTK-Thrsp-promoter-Fluc plasmi

pTK-Thrsp-promoter-Fluc plasmid

PVT107392

pTK-Thrsp-promoter-Fluc plasmid Information

Plasmid name: pTK-Thrsp(mouse)-promoter-Fluc
Alias:
Gene lenght: 2323
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: TK,Thrsp
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Thrsp-m-F2
Reverse primer:
Copy number: High
Induction:

pTK-Thrsp-promoter-Fluc plasmid Description

pTK-Thrsp-promoter-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pTK-Thrsp-promoter-Fluc plasmid Sequence

1 GGCCTAACTG GCCGGTACct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa 61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg 121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc 181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg 241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac 301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc 361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc 421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg 481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg 541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg 601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg 661 tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg 721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaG 781 GCTGTCCCTG CCTTGGTTTG AAGTCACCTG GGCTCAGGGC CAGCTGAGGT TACTTGGGGT 841 CTGGCAGCCC TGGTACCCCT TGCTCATCTT ACCCTTGGAA CACATTCACA TGATGTTCTG 901 GCCAGGCCCT TGACCCCAGT TCCTCTGAGG CCAATTCAAT TTGTCCAGCT CGGAGCTTTC 961 AGGGTGAGAT TAGTCCAGAA GACATGTCAG CCACCCTCAG TTTCTCCATG TGGCTGTAGT 1021 CATGGAAAGA CTAGAGAAAG TAAGTGTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 1081 TGTGTGTGTG TGTATGTGTG TATATATGTG TTTGTGTGTG TATGTGTGTG TATATGTGTG 1141 TTAGTGTATG TGTGTATATA TGTGTTTGTG TGTGTGTATG TGTGTATATG TGTGTTTGTG 1201 TATGCGTGTA TATATGTGTT AGTGTATGTG TATGTGTGTA TGTGTGTATA TGTGTGTTTG 1261 TGTATGTGTG TATATATGTG TTAGTGTATG TATGTATGTA TGTATGTATG TATGTATGTA 1321 TGTATGTGTG TATCTATGTG TGTTAGTGTG TGTATGTGTG TGTGCACACA GGGCCTCATC 1381 ATTGCCCTGT GGTGCTCTAC CTAGCTGAAG AGCTGTCTCT TCAGCCCCGA CTGGAGAAGT 1441 TACCTGAAGC CAGAGGACTG GTCAGCTTCG GACACTTCCT CCTTTCTGAG GTTTTCTGGC 1501 CAGTAGAATC AGATATGGCA TCTGTAAGAG CAGAAAAAAA TCCCGTGCCT CTGAAAAGAC 1561 TCTGTTTCCC TTTCCTGCCT CATTTCTTTC TCTAATCCTA GAGTTTTCCT GAATCCAAAT 1621 TTAGGACTAC TATCTCTCTT TCTGTGCCTG TGTGTGTACA TGTGTGTGTG CATGCATGCA 1681 AAGGTCAAAG GTCAACCTTT CACGTCATTC CTCAGGAACC ATCCCTCTCT ATCTATTTGA 1741 GATAGAGACT TTCTCTCTGG GACCTGGGGA ATCACTGATT CAGCTAGGCC GGCCAGTCCG 1801 ATAGCTCCAG GAACCCTCCT GTTTCCAGGT GTTCAGTGCT AGGATCACAA ACATAGACTA 1861 TTACACCCAG CTTTTTATGT GGGTGCTGGC GAGCACACTC AGGTCCGCAT GCAGCAATAA 1921 CATTACCAAC TGAGATATCC CCTCCCCCAG CTCAATCTAC CTTCTGACAT GCCACAAAGG 1981 CTGGTCTCAT TCTATCCCGG GTTTTCTGTC TGCCATGGAG ACCTGGAGTG CATCCTGGGC 2041 AGGAGTCTGC TGGCCGGGAC CTGGTGGTAC AGACACCTCT TAGTTATGGC TCCCATGTGT 2101 TCAGCACACT GGGCAGTGGT GGGCACACCT TTACTCCCAC CTCTCAGGAG GCAGAGGGAA 2161 GTAGATCTCT GTGGTCAAGG CCAGCCTGGT CTCCAGAGCA AGTTACGGGA CAGCAGTGCT 2221 CAAAAAGCCA GACCAAACAA AAACTCAAAC AGCAGCAACA ACAGCAACAA CAGCAAGCCT 2281 GTAAGCCAGA AGGGACGGGT ACTTGGGGAG CCCCCGCCCT TGGATCTAAT GTGCAGAGGC 2341 CTTAACATAT GACAGCCCCT GAGTTTTCTG ACTGCAGCAG AAGAGGCCCA GGAGTGACCT 2401 GGCCTCTGGG TCAGAGGCAG CAGGCAGCTG TTGCAGAGGC TCCGGGGCCT TTCTGGCTAT 2461 TGTCCATGGC ACGCTGGAGT CAGCCCTCCC AGACCTGACC GCAGCAGAAC ACTTTGTTTG 2521 CCAGTTCTCA CTTGGTGGCC CTAGGCTTGG GCACTAAGGC AGAGAGTAAT GCCCTTAGGG 2581 GCTTGAGTGT GGATTCAAAG GGAGTTACAT AACCAGGGCT GGAAAGGACT TCCTCTGGAG 2641 ATTTCCAAAC CTCTCGGTTT GTGAGTGGCA TCCTCCTGCC TCATGGCCTT GGGCTGGATG 2701 CACTGCGCAG AGTCAGCCCT GGAAGAATCT CTCCCCAGAA AATGTAAGAA ACCATGATAC 2761 AGGGCTAGCA ATGGATCAAT AGAACACCAA TTAAAAGGGC ACTTTTTGCT CTCTCATAGG 2821 ACCTGCACCC ACATGGTATC TTGAAACATC TGTAAACTCC CTTCCCCTTT CCTGATCTCT 2881 GCAGGCACCA GGCACACGAG GTGCACAGAC ATACAGACAG GCAAAACATT CACATATATA 2941 AAATAAGATA TTTAAAAAAA CAACCAGCCA AAAACCTTAT TTATTTATTT ATTTATTTAT 3001 TTATTTATTT ATCTATTTAT TTTGATGAGA TCAAGTACAG AGCCCCTTAC ATGTTGTGAA 3061 TCCCACCCAC CACTGAGCTA CATCTTCTGG CGCCTTAATT GAaAGCTTGG CAATCCGGTA 3121 CTGTTGGTAA AGCCACCATG GAAGATGCCA AAAACATTAA GAAGGGCCCA GCGCCATTCT 3181 ACCCACTCGA AGACGGGACC GCCGGCGAGC AGCTGCACAA AGCCATGAAG CGCTACGCCC 3241 TGGTGCCCGG CACCATCGCC TTTACCGACG CACATATCGA GGTGGACATT ACCTACGCCG 3301 AGTACTTCGA GATGAGCGTT CGGCTGGCAG AAGCTATGAA GCGCTATGGG CTGAATACAA 3361 ACCATCGGAT CGTGGTGTGC AGCGAGAATA GCTTGCAGTT CTTCATGCCC GTGTTGGGTG 3421 CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG CTAACGACAT CTACAACGAG CGCGAGCTGC 3481 TGAACAGCAT GGGCATCAGC CAGCCCACCG TCGTATTCGT GAGCAAGAAA GGGCTGCAAA 3541 AGATCCTCAA CGTGCAAAAG AAGCTACCGA TCATACAAAA GATCATCATC ATGGATAGCA 3601 AGACCGACTA CCAGGGCTTC CAAAGCATGT ACACCTTCGT GACTTCCCAT TTGCCACCCG 3661 GCTTCAACGA GTACGACTTC GTGCCCGAGA GCTTCGACCG GGACAAAACC ATCGCCCTGA 3721 TCATGAACAG TAGTGGCAGT ACCGGATTGC CCAAGGGCGT AGCCCTACCG CACCGCACCG 3781 CTTGTGTCCG ATTCAGTCAT GCCCGCGACC CCATCTTCGG CAACCAGATC ATCCCCGACA 3841 CCGCTATCCT CAGCGTGGTG CCATTTCACC ACGGCTTCGG CATGTTCACC ACGCTGGGCT 3901 ACTTGATCTG CGGCTTTCGG GTCGTGCTCA TGTACCGCTT CGAGGAGGAG CTATTCTTGC 3961 GCAGCTTGCA AGACTATAAG ATTCAATCTG CCCTGCTGGT GCCCACACTA TTTAGCTTCT 4021 TCGCTAAGAG CACTCTCATC GACAAGTACG ACCTAAGCAA CTTGCACGAG ATCGCCAGCG 4081 GCGGGGCGCC GCTCAGCAAG GAGGTAGGTG AGGCCGTGGC CAAACGCTTC CACCTACCAG 4141 GCATCCGCCA GGGCTACGGC CTGACAGAAA CAACCAGCGC CATTCTGATC ACCCCCGAAG 4201 GGGACGACAA GCCTGGCGCA GTAGGCAAGG TGGTGCCCTT CTTCGAGGCT AAGGTGGTGG 4261 ACTTGGACAC CGGTAAGACA CTGGGTGTGA ACCAGCGCGG CGAGCTGTGC GTCCGTGGCC 4321 CCATGATCAT GAGCGGCTAC GTTAACAACC CCGAGGCTAC AAACGCTCTC ATCGACAAGG 4381 ACGGCTGGCT GCACAGCGGC GACATCGCCT ACTGGGACGA GGACGAGCAC TTCTTCATCG 4441 TGGACCGGCT GAAGAGCCTG ATCAAATACA AGGGCTACCA GGTAGCCCCA GCCGAACTGG 4501 AGAGCATCCT GCTGCAACAC CCCAACATCT TCGACGCCGG GGTCGCCGGC CTGCCCGACG 4561 ACGATGCCGG CGAGCTGCCC GCCGCAGTCG TCGTGCTGGA ACACGGTAAA ACCATGACCG 4621 AGAAGGAGAT CGTGGACTAT GTGGCCAGCC AGGTTACAAC CGCCAAGAAG CTGCGCGGTG 4681 GTGTTGTGTT CGTGGACGAG GTGCCTAAAG GACTGACCGG CAAGTTGGAC GCCCGCAAGA 4741 TCCGCGAGAT TCTCATTAAG GCCAAGAAGG GCGGCAAGAT CGCCGTGTAA TAATTCTAGA 4801 GTCGGGGCGG CCGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA 4861 CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT 4921 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA 4981 TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT 5041 GTGGTAAAAT CGATAAGGAT CCGTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC 5101 TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC 5161 ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 5221 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 5281 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 5341 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 5401 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 5461 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 5521 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 5581 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 5641 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 5701 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 5761 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 5821 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 5881 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 5941 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 6001 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 6061 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 6121 TTGGTCTGAC AGCGGCCGCA AATGCTAAAC CACTGCAGTG GTTACCAGTG CTTGATCAGT 6181 GAGGCACCGA TCTCAGCGAT CTGCCTATTT CGTTCGTCCA TAGTGGCCTG ACTCCCCGTC 6241 GTGTAGATCA CTACGATTCG TGAGGGCTTA CCATCAGGCC CCAGCGCAGC AATGATGCCG 6301 CGAGAGCCGC GTTCACCGGC CCCCGATTTG TCAGCAATGA ACCAGCCAGC AGGGAGGGCC 6361 GAGCGAAGAA GTGGTCCTGC TACTTTGTCC GCCTCCATCC AGTCTATGAG CTGCTGTCGT 6421 GATGCTAGAG TAAGAAGTTC GCCAGTGAGT AGTTTCCGAA GAGTTGTGGC CATTGCTACT 6481 GGCATCGTGG TATCACGCTC GTCGTTCGGT ATGGCTTCGT TCAACTCTGG TTCCCAGCGG 6541 TCAAGCCGGG TCACATGATC ACCCATATTA TGAAGAAATG CAGTCAGCTC CTTAGGGCCT 6601 CCGATCGTTG TCAGAAGTAA GTTGGCCGCG GTGTTGTCGC TCATGGTAAT GGCAGCACTA 6661 CACAATTCTC TTACCGTCAT GCCATCCGTA AGATGCTTTT CCGTGACCGG CGAGTACTCA 6721 ACCAAGTCGT TTTGTGAGTA GTGTATACGG CGACCAAGCT GCTCTTGCCC GGCGTCTATA 6781 CGGGACAACA CCGCGCCACA TAGCAGTACT TTGAAAGTGC TCATCATCGG GAATCGTTCT 6841 TCGGGGCGGA AAGACTCAAG GATCTTGCCG CTATTGAGAT CCAGTTCGAT ATAGCCCACT 6901 CTTGCACCCA GTTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCGGG GTGTGCAAAA 6961 ACAGGCAAGC AAAATGCCGC AAAGAAGGGA ATGAGTGCGA CACGAAAATG TTGGATGCTC 7021 ATACTCGTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTACTAGTA CGTCTCTCAA 7081 GGATAAGTAA GTAATATTAA GGTACGGGAG GTATTGGACA GGCCGCAATA AAATATCTTT 7141 ATTTTCATTA CATCTGTGTG TTGGTTTTTT GTGTGAATCG ATAGTACTAA CATACGCTCT 7201 CCATCAAAAC AAAACGAAAC AAAACAAACT AGCAAAATAG GCTGTCCCCA GTGCAAGTGC 7261 AGGTGCCAGA ACATTTCTCT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.