pTac-GST-TP53-S371A-K373A plas

pTac-GST-TP53-S371A-K373A plasmid

PVT107383

pTac-GST-TP53-S371A-K373A plasmid Information

Plasmid name: pTac-GST-TP53(human)-S371A-K373A
Alias: NM_000546.6;BCC7; BMFS5; LFS1; P53; TRP53;
Gene lenght: 1182
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pGEX-5'
Reverse primer:
Copy number: Middle
Induction: IPTG Induction

pTac-GST-TP53-S371A-K373A plasmid Description

pTac-GST-TP53-S371A-K373A is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-TP53-S371A-K373A plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatgga ggagccgcag tcagatccta 961 gcgtcgagcc ccctctgagt caggaaacat tttcagacct atggaaacta cttcctgaaa 1021 acaacgttct gtcccccttg ccgtcccaag caatggatga tttgatgctg tccccggacg 1081 atattgaaca atggttcact gaagacccag gtccagatga agctcccaga atgccagagg 1141 ctgctccccc cgtggcccct gcaccagcag ctcctacacc ggcggcccct gcaccagccc 1201 cctcctggcc cctgtcatct tctgtccctt cccagaaaac ctaccagggc agctacggtt 1261 tccgtctggg cttcttgcat tctgggacag ccaagtctgt gacttgcacg tactcccctg 1321 ccctcaacaa gatgttttgc caactggcca agacctgccc tgtgcagctg tgggttgatt 1381 ccacaccccc gcccggcacc cgcgtccgcg ccatggccat ctacaagcag tcacagcaca 1441 tgacggaggt tgtgaggcgc tgcccccacc atgagcgctg ctcagatagc gatggtctgg 1501 cccctcctca gcatcttatc cgagtggaag gaaatttgcg tgtggagtat ttggatgaca 1561 gaaacacttt tcgacatagt gtggtggtgc cctatgagcc gcctgaggtt ggctctgact 1621 gtaccaccat ccactacaac tacatgtgta acagttcctg catgggcggc atgaaccgga 1681 ggcccatcct caccatcatc acactggaag actccagtgg taatctactg ggacggaaca 1741 gctttgaggt gcgtgtttgt gcctgtcctg ggagagaccg gcgcacagag gaagagaatc 1801 tccgcaagaa aggggagcct caccacgagc tgcccccagg gagcactaag cgagcactgc 1861 ccaacaacac cagctcctct ccccagccaa agaagaaacc actggatgga gaatatttca 1921 cccttcagat ccgtgggcgt gagcgcttcg agatgttccg agagctgaat gaggccttgg 1981 aactcaagga tgcccaggct gggaaggagc caggggggag cagggctcac tccagccacc 2041 tgaagGcGaa aGCgggtcag tctacctccc gccataaaaa actcatgttc aagacagaag 2101 ggcctgactc agactgactc gagcggccgc atcgtgactg actgacgatc tgcctcgcgc 2161 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 2221 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 2281 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat aattcttgaa 2341 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 2401 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 2461 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 2521 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 2581 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 2641 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 2701 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2761 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 2821 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2881 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2941 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 3001 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 3061 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg 3121 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 3181 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 3241 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 3301 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 3361 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 3421 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 3481 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 3541 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 3601 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 3661 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 3721 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3781 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3841 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3901 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3961 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 4021 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 4081 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 4141 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 4201 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 4261 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 4321 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg 4381 gtatttcaca ccgcataaat tccgacacca tcgaatggtg caaaaccttt cgcggtatgg 4441 catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat 4501 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg 4561 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt 4621 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg 4681 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg 4741 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct 4801 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc 4861 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat 4921 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta 4981 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg 5041 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc 5101 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc 5161 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg 5221 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg 5281 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa 5341 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac 5401 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa 5461 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 5521 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 5581 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 5641 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 5701 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 5761 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 5821 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc 5881 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt 5941 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtaac 6001 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc 6061 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga 6121 tggcgttgga att

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.