pCMV-FBXO32-V5-Neo plasmid

pCMV-FBXO32-V5-Neo plasmid

PVT107100

pCMV-FBXO32-V5-Neo plasmid Information

Plasmid name: pCMV-FBXO32(human)-V5-Neo
Alias: NM_058229.4;Fbx32; MAFbx
Gene lenght: 1065
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-FBXO32-V5-Neo plasmid Description

pCMV-FBXO32-V5-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-FBXO32-V5-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaGC 901 CACCatgcca ttcctcgggc aggactggcg gtcccccggg cagaactggg tgaagacggc 961 cgacggctgg aagcgcttcc tggatgagaa gagcggcagt ttcgtgagcg acctcagcag 1021 ttactgcaac aaggaggtat acaataagga gaatcttttc aacagcctga actatgatgt 1081 tgcagccaag aagagaaaga aggacatgct gaatagcaaa accaaaactc agtatttcca 1141 ccaagaaaaa tggatctatg ttcacaaagg aagtactaaa gagcgccatg gatattgcac 1201 cctgggggaa gctttcaaca gactggactt ctcaactgcc attctggatt ccagaagatt 1261 taactacgtg gtccggctgt tggagctgat agcaaagtca cagctcacat ccctgagtgg 1321 catcgcccaa aagaacttca tgaatatttt ggaaaaagtg gtactgaaag tccttgaaga 1381 ccagcaaaac attagactaa taagggaact actccagacc ctctacacat ccttatgtac 1441 actggtccaa agagtcggca agtctgtgct ggtcgggaac attaacatgt gggtgtatcg 1501 gatggagacg attctccact ggcagcagca gctgaacaac attcagatca ccaggcctgc 1561 cttcaaaggc ctcaccttca ctgacctgcc tttgtgccta caactgaaca tcatgcagag 1621 gctgagcgac gggcgggacc tggtcagcct gggccaggct gcccccgacc tgcacgtgct 1681 cagcgaagac cggctgctgt ggaagaaact ctgccagtac cacttctccg agcggcagat 1741 ccgcaaacga ttaattctgt cagacaaagg gcagctggat tggaagaaga tgtatttcaa 1801 acttgtccga tgttacccaa ggaaagagca gtatggagat acccttcagc tctgcaaaca 1861 ctgtcacatc ctttcctgga agggcactga ccatccgtgc actgccaata acccagagag 1921 ctgctccgtt tcactttcac cccaggactt tatcaacttg ttcaagttcg gtggaggcgg 1981 ttcaggtaag cctatcccta accctctcct cggtctcgat tctacatagg atccactagt 2041 ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc 2101 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 2161 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 2221 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 2281 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 2341 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 2401 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 2461 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 2521 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 2581 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 2641 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 2701 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 2761 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 2821 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 2881 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 2941 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 3001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 3061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct 3121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg 3181 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat 3241 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 3301 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 3361 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 3421 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 3481 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 3541 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 3601 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 3661 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 3721 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 3781 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 3841 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 3901 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 3961 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 4021 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca 4081 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 4141 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 4201 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 4261 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 4321 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag 4381 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 4441 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 4501 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 4561 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 4621 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 4681 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 4741 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 4801 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 4861 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 4921 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 4981 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 5041 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 5101 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 5161 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 5221 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 5281 tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg 5341 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 5401 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 5461 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 5521 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 5581 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 5641 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 5701 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 5761 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 5821 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 5881 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 5941 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 6001 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 6061 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 6121 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 6181 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 6241 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 6301 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 6361 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 6421 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 6481 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.