pLV2-CMV-SQSTM1-3×Myc-Puro pla

pLV2-CMV-SQSTM1-3×Myc-Puro plasmid

PVT107046

pLV2-CMV-SQSTM1-3×Myc-Puro plasmid Information

Plasmid name: pLV2-CMV-SQSTM1(human)-3×Myc-Puro
Alias: NM_003900.5;DMRV; EBIAP; FTDALS3; NADGP; OSIL;p60; p62; p62B; PDB3; ZIP3
Gene lenght: 1320
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-SQSTM1-3×Myc-Puro plasmid Description

pLV2-CMV-SQSTM1-3×Myc-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV2-CMV-SQSTM1-3×Myc-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 GCCACCatgg cgtcgctcac cgtgaaggcc taccttctgg gcaaggagga cgcggcgcgc 2881 gagattcgcc gcttcagctt ctgctgcagc cccgagcctg aggcggaagc cgaggctgcg 2941 gcgggtccgg gaccctgcga gcggctgctg agccgggtgg ccgccctgtt ccccgcgctg 3001 cggcctggcg gcttccaggc gcactaccgc gatgaggacg gggacttggt tgccttttcc 3061 agtgacgagg aattgacaat ggccatgtcc tacgtgaagg atgacatctt ccgaatctac 3121 attaaagaga aaaaagagtg ccggcgggac caccgcccac cgtgtgctca ggaggcgccc 3181 cgcaacatgg tgcaccccaa tgtgatctgc gatggctgca atgggcctgt ggtaggaacc 3241 cgctacaagt gcagcgtctg cccagactac gacttgtgta gcgtctgcga gggaaagggc 3301 ttgcaccggg ggcacaccaa gctcgcattc cccagcccct tcgggcacct gtctgagggc 3361 ttctcgcaca gccgctggct ccggaaggtg aaacacggac acttcgggtg gccaggatgg 3421 gaaatgggtc caccaggaaa ctggagccca cgtcctcctc gtgcagggga ggcccgccct 3481 ggccccacgg cagaatcagc ttctggtcca tcggaggatc cgagtgtgaa tttcctgaag 3541 aacgttgggg agagtgtggc agctgccctt agccctctgg gcattgaagt tgatatcgat 3601 gtggagcacg gagggaaaag aagccgcctg acccccgtct ctccagagag ttccagcaca 3661 gaggagaaga gcagctcaca gccaagcagc tgctgctctg accccagcaa gccgggtggg 3721 aatgttgagg gcgccacgca gtctctggcg gagcagatga ggaagatcgc cttggagtcc 3781 gaggggcgcc ctgaggaaca gatggagtcg gataactgtt caggaggaga tgatgactgg 3841 acccatctgt cttcaaaaga agtggacccg tctacaggtg aactccagtc cctacagatg 3901 ccagaatccg aagggccaag ctctctggac ccctcccagg agggacccac agggctgaag 3961 gaagctgcct tgtacccaca tctcccgcca gaggctgacc cgcggctgat tgagtccctc 4021 tcccagatgc tgtccatggg cttctctgat gaaggcggct ggctcaccag gctcctgcag 4081 accaagaact atgacatcgg agcggctctg gacaccatcc agtattcaaa gcatcccccg 4141 ccgttggaat tcGAACAAAA ACTAATATCA GAGGAAGATT TGGAACAGAA ACTAATCAGT 4201 GAAGAAGATT TAGAGCAAAA GTTAATTTCC GAGGAGGACT TATAGtgcag tcgacggtac 4261 cgcgggcccg ggatcccgcg actctagata attctaccgg gtaggggagg cgcttttccc 4321 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg 4381 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg 4441 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca 4501 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat 4561 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct 4621 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag 4681 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac 4741 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac 4801 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 4861 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 4921 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 4981 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 5041 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5101 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5161 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5221 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5281 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5341 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 5401 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga 5461 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 5521 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 5581 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 5641 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 5701 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 5761 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 5821 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 5881 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 5941 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 6001 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga 6061 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa 6121 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac 6181 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt 6241 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc 6301 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg 6361 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac 6421 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta 6481 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag 6541 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca 6601 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca 6661 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 6721 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 6781 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 6841 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 6901 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga 6961 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 7021 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 7081 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 7141 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 7201 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 7261 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 7321 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 7381 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 7441 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 7501 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 7561 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 7621 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 7681 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7741 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7801 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7861 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7921 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7981 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 8041 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 8101 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 8161 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 8221 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 8281 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 8341 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 8401 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 8461 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 8521 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 8581 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 8641 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 8701 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8761 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8821 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8881 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8941 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 9001 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 9061 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 9121 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct 9181 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 9241 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac 9301 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact 9361 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt 9421 taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta 9481 tttgttaaat atgtactaca aacttagtag t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.