pCMV-GAP43-EYFP-Neo plasmid

pCMV-GAP43-EYFP-Neo plasmid

PVT106935

pCMV-GAP43-EYFP-Neo plasmid Information

Plasmid name: pCMV-GAP43(human)-EYFP-Neo
Alias: NM_002045.4;B-50; GAP-43; PP46
Gene lenght: 714
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Yellow
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-GAP43-EYFP-Neo plasmid Description

pCMV-GAP43-EYFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-GAP43-EYFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcGCCACC atgctgtgct gtatgagaag 661 aaccaaacag gttgaaaaaa atgatgacga ccaaaagatt gaacaagatg gtatcaaacc 721 agaagataaa gctcataagg ccgcaaccaa aattcaggct agcttccgtg gacacataac 781 aaggaaaaag ctcaaaggag agaagaagga tgatgtccaa gctgctgagg ctgaagctaa 841 taagaaggat gaagcccctg ttgccgatgg ggtggagaag aagggagaag gcaccactac 901 tgccgaagca gccccagcca ctggctccaa gcctgatgag cccggcaaag caggagaaac 961 tccttccgag gagaagaagg gggagggtga tgctgccaca gagcaggcag ccccccaggc 1021 tcctgcatcc tcagaggaga aggccggctc agctgagaca gaaagtgcca ctaaagcttc 1081 cactgataac tcgccgtcct ccaaggctga agatgcccca gccaaggagg agcctaaaca 1141 agccgatgtg cctgctgctg tcactgctgc tgctgccacc acccctgccg cagaggatgc 1201 tgctgccaag gcaacagccc agcctccaac ggagactggg gagagcagcc aagctgaaga 1261 gaacatagaa gctgtagatg aaaccaaacc taaggaaagt gcccggcagg acgagggtaa 1321 agaagaggaa cctgaggctg accaagaaca tgccggtgga ggcggatcag gaggcggtgg 1381 atctacggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt 1441 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 1501 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 1561 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt 1621 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 1681 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 1741 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 1801 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 1861 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 1921 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 1981 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc 2041 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 2101 cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc 2161 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa 2221 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa 2281 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 2341 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt 2401 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg 2461 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg 2521 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta 2581 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg 2641 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa 2701 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct 2761 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct 2821 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 2881 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 2941 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag 3001 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 3061 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 3121 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 3181 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 3241 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 3301 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat 3361 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 3421 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 3481 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga 3541 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 3601 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 3661 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 3721 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 3781 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 3841 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga 3901 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 3961 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 4021 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 4081 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 4141 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca 4201 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 4261 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 4321 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg 4381 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg 4441 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac 4501 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc 4561 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag 4621 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 4681 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 4741 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 4801 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 4861 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg 4921 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 4981 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 5041 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 5101 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 5161 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 5221 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 5281 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 5341 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 5401 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.